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Development of novel staining method to enable 3D pathological diagnosis.

Research Project

Project/Area Number 21K06903
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49020:Human pathology-related
Research InstitutionOsaka University

Principal Investigator

Nojima Satoshi  大阪大学, 大学院医学系研究科, 准教授 (40528791)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords3次元イメージング / 組織透明化 / 臨床病理診断 / 炎症性腸疾患 / 潰瘍性大腸炎 / 新規診断技術 / 人工知能 / 機械学習 / 甲状腺腫瘍 / 尿路系腫瘍 / 診断病理学 / 3次元イメージング
Outline of Research at the Start

本研究は、最先端イメージング技術CUBICをヒト病理組織診断に実践的に応用することを目標とするものであり、特に、臨床病理診断におけるPAS染色やレクチン染色といった所謂“特殊染色”に該当するような新たな蛍光染色法を開発し、現在の2次元の評価では難しいような診断を可能とする新規イメージング系を確立することをその主軸に置く。
これらの新規技術により従来の方法では難しかった疾患の鑑別が可能となれば、病理診断技術の発展に大きく寄与する可能性がある。

Outline of Final Research Achievements

Although the utility of tissue clearing and three-dimensional imaging techniques in clinical histopathology has been established, further methodological developments are required for their use in clinical practice. We developed a novel periodic acid-FAM hydrazide staining method (PAFhy stain), which is based on a principle similar to the classical PAS stain. This method could be applied to 3D imaging with a tissue-clearing technique; it enabled clear and detailed imaging of the 3D architecture of crypts in human colorectal mucosae. The 3D imaging also revealed the 3D distributions of neutrophils in cryptitis and crypt abscesses in ulcerative colitis tissues. Quantitative analysis of crypts based on 3D morphological changes enabled differential diagnosis among ulcerative colitis, Crohn's disease, and non-inflammatory bowel disease colitis. Therefore, PAFhy staining and the 3D imaging with it are promising approaches for next-generation experimental and clinical histopathology.

Academic Significance and Societal Importance of the Research Achievements

本研究で新たに開発されたPAFhy stain法は、3次元病理組織学における特殊染色に位置する有用な手法であり、基礎病理学・臨床病理学の新たな基盤的技術となりえるものである。これらがより標準化され他の技術と組み合わされることで新たなプラットフォームが確立できる可能性があり、これを元に疾患に根差した多くの病理学的研究、臨床的研究が蓄積されることで、新たな診断基準・臨床予測法の確立、新規治療法の開発に繋がることが期待できる。

Report

(3 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • Research Products

    (9 results)

All 2024 2023 2022

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (2 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Identification of invasive subpopulations using spatial transcriptome analysis in thyroid follicular tumors2024

    • Author(s)
      Suzuki Ayana、Nojima Satoshi、Tahara Shinichiro、Motooka Daisuke、Kohara Masaharu、Okuzaki Daisuke、Hirokawa Mitsuyoshi、Morii Eiichi
    • Journal Title

      Journal of Pathology and Translational Medicine

      Volume: 58 Issue: 1 Pages: 22-28

    • DOI

      10.4132/jptm.2023.11.21

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deep Learning-Based Differential Diagnosis of Follicular Thyroid Tumors Using Histopathological Images2023

    • Author(s)
      Nojima Satoshi、Kadoi Tokimu、Suzuki Ayana、Kato Chiharu、Ishida Shoichi、Kido Kansuke、Fujita Kazutoshi、Okuno Yasushi、Hirokawa Mitsuyoshi、Terayama Kei、Morii Eiichi
    • Journal Title

      Modern Pathology

      Volume: 36 Issue: 11 Pages: 100296-100296

    • DOI

      10.1016/j.modpat.2023.100296

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ovarian high‐grade serous carcinoma cells with low SMARCA4 expression and high SMARCA2 expression contribute to platinum resistance2023

    • Author(s)
      Kido Kansuke、Nojima Satoshi、Motooka Daisuke、Nomura Yusuke、Kohara Masaharu、Sato Kazuaki、Ohshima Kenji、Tahara Shinichiro、Kurashige Masako、Umeda Daisuke、Takashima Tsuyoshi、Kiyokawa Hiroki、Ukon Koto、Matsui Takahiro、Okuzaki Daisuke、Morii Eiichi
    • Journal Title

      The Journal of Pathology

      Volume: 260 Issue: 1 Pages: 56-70

    • DOI

      10.1002/path.6064

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Class IV semaphorins in disease pathogenesis2022

    • Author(s)
      Nojima Satoshi
    • Journal Title

      Pathology International

      Volume: 72 Issue: 10 Pages: 471-487

    • DOI

      10.1111/pin.13270

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Semaphorin 6D?expressing mesenchymal cells regulate IL-10 production by ILC2s in the lung2022

    • Author(s)
      Naito Maiko、Nakanishi Yoshimitsu、Motomura Yasutaka、Takamatsu Hyota、Koyama Shohei、Nishide Masayuki、Naito Yujiro、Izumi Mayuko、Mizuno Yumiko、Yamaguchi Yuta、Nojima Satoshi、Okuzaki Daisuke、Kumanogoh Atsushi
    • Journal Title

      Life Science Alliance

      Volume: 5 Issue: 11 Pages: 1486-1486

    • DOI

      10.26508/lsa.202201486

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Novel Three-Dimensional Imaging System Based on Polysaccharide Staining for Accurate Histopathological Diagnosis of Inflammatory Bowel Diseases2022

    • Author(s)
      Nojima Satoshi、Ishida Shoichi、Terayama Kei、Matsumoto Katsuhiko、Matsui Takahiro、Tahara Shinichiro、Ohshima Kenji、Kiyokawa Hiroki、Kido Kansuke、Ukon Koto、Yoshida Shota Y.、Mitani Tomoki T.、Doki Yuichiro、Mizushima Tsunekazu、Okuno Yasushi、Susaki Etsuo A.、Ueda Hiroki R.、Morii Eiichi
    • Journal Title

      Cellular and Molecular Gastroenterology and Hepatology

      Volume: 14 Issue: 4 Pages: 905-924

    • DOI

      10.1016/j.jcmgh.2022.07.001

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 組織透明化による3次元イメージング技術を用いた空間的解析2024

    • Author(s)
      野島聡
    • Organizer
      第113回 日本病理学会総会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 組織病理学に寄与する先端イメージング技術2023

    • Author(s)
      野島聡
    • Organizer
      第112回 日本病理学会総会・特別企画:病理専門医が得られる研究マインド
    • Related Report
      2022 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 組織又は細胞中のグリコール基を含む物質を特異的に染色する蛍光染色方法2022

    • Inventor(s)
      野島聡 他
    • Industrial Property Rights Holder
      野島聡 他
    • Industrial Property Rights Type
      特許
    • Filing Date
      2022
    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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