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Molecular mechanism of complete statin intolerance in Japanese

Research Project

Project/Area Number 21K08139
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 53020:Cardiology-related
Research InstitutionKanazawa Medical University

Principal Investigator

梶波 康二  金沢医科大学, 医学部, 教授 (40262563)

Co-Investigator(Kenkyū-buntansha) 竹田 健史  金沢医科大学, 医学部, 助教 (90340009)
Project Period (FY) 2021-04-01 – 2025-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsスタチン / 筋障害 / 遺伝子異常 / 不耐
Outline of Research at the Start

動脈硬化性心血管イベントの初発・再発予防に必須な薬剤であるスタチンが継続服用困難(不耐)となるメカニズム解明をリサーチクエスチョンとし、extreme phenotypeである完全不耐例に焦点を絞って、全エクソン塩基配列の決定、ゲノムワイド多型連鎖解析を用いた先行研究で得られた連鎖多型が存在する遺伝子における非翻訳領域を含むディープシークエンシング、保存血清を用いた液性因子としてのリピドーム網羅的解析、および患者細胞を用いた細胞生物学的機能解析、を行う。並行して、スタチン長期服用でも全く筋障害を認めていない真の対照例にも同様の解析を行い、知見の病態特異性ならびに人種特異性の検証を目指す。

Outline of Annual Research Achievements

1)スタチン完全不耐例の遺伝子解析:前年度までに同意取得済の完全不耐2例に加え、令和5年度新たに診断し研究参加の同意が得られた完全不耐1例、の計3例を対象に、末梢血を採取し全エクソームシークエンシングを行った。研究当初は自施設での解析を予定していたが、COVID-19蔓延の影響を受け外部解析機関への委託に切り替え、年度前半に委託先を選定し、後半に契約さらには解析実施に至った。
解析結果を入手後は、次世代シークエンサーを用いた解析結果と、健常日本人由来の公表されたデータベースとの突合を、以下の段階的手法にて進行中である;①nonsense変異の検出、②missense変異→機能的変化の可能性検討、③スタチン薬物代謝関連遺伝子、細胞内コレステロール代謝関連遺伝子、を候補として、それらの高頻度多型の組み合わせとの連鎖検討。

2)リピドーム解析:長期(2年以上)のスタチン服薬にも拘らず筋障害を発症しない「真の対照例」について、血中脂肪酸網羅的測定を行ったが、年齢・性別をマッチさせたスタチン非服用者ならびに正常対照者との間に有意な差を認めなかった。

3)プロテオーム解析:リピドーム解析の結果を踏まえて解析を実施する予定であったが、前項の記載通りこの段階には到達できなかった。

Current Status of Research Progress
Current Status of Research Progress

4: Progress in research has been delayed.

Reason

遺伝子解析を外部解析機関に発注し完了したが、その結果と日本人健常人データベースの比較作業中に能登半島地震が発生し、その被害対応に追われ研究進捗が頓挫した。

Strategy for Future Research Activity

全エクソン塩基配列解析結果と健常日本人データベースとの突合を進めており、変異部分の洗い出し、その病的意義の吟味、に進む予定である。

Report

(3 results)
  • 2023 Research-status Report
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (18 results)

All 2024 2023 2022 2021

All Journal Article (13 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 12 results,  Open Access: 6 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results) Book (2 results)

  • [Journal Article] Efficacy, Safety, and Pharmacokinetics of Inclisiran in Japanese Patients: Results from ORION-152024

    • Author(s)
      Yamashita Shizuya、Kiyosue Arihiro、Maheux Pierre、Mena-Madrazo Jorge、Lesogor Anastasia、Shao Qing、Tamaki Yuko、Nakamura Hidekazu、Akahori Mizuki、Kajinami Kouji
    • Journal Title

      Journal of Atherosclerosis and Thrombosis

      Volume: 31 Issue: 6 Pages: 876-903

    • DOI

      10.5551/jat.64454

    • ISSN
      1340-3478, 1880-3873
    • Year and Date
      2024-06-01
    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thrombotic Microangiopathy after a 15-year Treatment with Interferon Beta-1b in a Patient with Multiple Sclerosis: A Case Report and Review of Literature2024

    • Author(s)
      Akita Satori、Fujibayashi Kosuke、Ueno Ei-ichi、Wakasa Minoru、Kawai Yasuyuki、Kajinami Kouji
    • Journal Title

      Internal Medicine

      Volume: 63 Issue: 8 Pages: 1113-1117

    • DOI

      10.2169/internalmedicine.1846-23

    • ISSN
      0918-2918, 1349-7235
    • Year and Date
      2024-04-15
    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Marchiafava‐Bignami disease: Prompt diagnosis made by magnetic resonance brain imaging2024

    • Author(s)
      Akita Satori、Takakuwa Takeshi、Kajinami Kouji
    • Journal Title

      Clinical Case Reports

      Volume: 12 Issue: 4

    • DOI

      10.1002/ccr3.8787

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Novel Approach: Investigating the Intracellular Clearance Mechanism of Glyceraldehyde-Derived Advanced Glycation End-Products Using the Artificial Checkpoint Kinase 1 d270KD Mutant as a Substrate Model2023

    • Author(s)
      Takeda Kenji、Sakai-Sakasai Akiko、Kajinami Kouji、Takeuchi Masayoshi
    • Journal Title

      Cells

      Volume: 12 Issue: 24 Pages: 2838-2838

    • DOI

      10.3390/cells12242838

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thrombotic Risk Stratification and Intensive Statin Therapy for Secondary Prevention of Coronary Artery Disease ― Insights From the REAL-CAD Study ―2022

    • Author(s)
      Natsuaki M, Morimoto T, Iimuro S, Nagai R, Kimura T, the REAL-CAD Investigators (Kajinami K, et al), et al..
    • Journal Title

      Circulation Journal

      Volume: 86 Issue: 9 Pages: 1416-1427

    • DOI

      10.1253/circj.CJ-22-0315

    • ISSN
      1346-9843, 1347-4820
    • Year and Date
      2022-08-25
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Small interfering RNA to reduce lipoprotein(a) in cardiovascular disease2022

    • Author(s)
      O’Donoghue ML, Rosenson RS, Gencer B, Lopez JAG, Lepor NE, Baum SJ, Stout E, Gaudet D, Knusel B, Kuder JF, Ran X, Murphy SA, Wang H, Wu Y, Kassahun H, Sabatine MS, the OCEAN(a)-DOSE Trial Investigators (Kajinami K, et al.).
    • Journal Title

      N Engl J Med

      Volume: 387 Issue: 20 Pages: 1855-1864

    • DOI

      10.1056/nejmoa2211023

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Increased plasma glutamate in non-smokers with vasospastic angina pectoris is associated with plasma cystine and antioxidant capacity2022

    • Author(s)
      Oda M, Fujibayashi K, Wakasa M, Takano S, Fujita W, Kitayama M, Nakanishi H, Saito K, Kawai Y, Kajinami K
    • Journal Title

      Scand Cardiovasc J

      Volume: 56 Issue: 1 Pages: 180-186

    • DOI

      10.1080/14017431.2022.2085884

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Glyceraldehyde-derived advanced glycation end-products are associated with the severity of cardiac function and heart failure in patients with diabetic cardiomyopathy2022

    • Author(s)
      Yasuda Y, Aoki H, Wakasa M, Fujita W, Kawai Y, Kajinami K
    • Journal Title

      Scand Cardiovasc J

      Volume: 56 Issue: 1 Pages: 208-216

    • DOI

      10.1080/14017431.2022.2095013

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Response to pre-dilatation with plain old balloons can predict target lesion revascularization after drug coated balloon angioplasty for de novo small coronary artery lesions.2022

    • Author(s)
      Takamura T, Kawai Y, Akita S, Oda M, Akao H, Nakagawa T, Takama S, Tsuchiya T, Kitayama M, Kajinami K.
    • Journal Title

      J Int Med Res

      Volume: 50 Issue: 8 Pages: 0605221113779-0605221113779

    • DOI

      10.1177/03000605221113779

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The circulating furin-cleaved/mature PCSK9 ratio has a potential prognostic significance in statin-naive patients with acute ST elevation myocardial infarction2022

    • Author(s)
      Sawaguchi J, Saeki Y, Oda M, Takamura TA, Fujibayashi K, Wakasa M, Akao H, Kitayama M, Kawai Y, Kajinami K.
    • Journal Title

      Atherosclerosis Plus

      Volume: 50 Pages: 50-56

    • DOI

      10.1016/j.athplu.2022.09.002

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A case of myopericarditis after the second dose of mRNA COVID-19 vaccine in a patient with a history of myopericarditis2022

    • Author(s)
      Fujibayashi K, Kajinami K
    • Journal Title

      Clin Med Insights Case Rep

      Volume: 15 Pages: 95476221138648-95476221138648

    • DOI

      10.1177/11795476221138648

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cardiac Myosin Activation with Omecamtiv Mecarbil in Systolic Heart Failure2021

    • Author(s)
      Teerlink JR, Diaz R, Felker GM, McMurray JJV, GALACTIC-HF Investigators (Kajinami K, et al), et al.
    • Journal Title

      New England Journal of Medicine

      Volume: 384 Issue: 2 Pages: 105-116

    • DOI

      10.1056/nejmoa2025797

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] スタチン不耐の疫学と対策2021

    • Author(s)
      梶波康二
    • Journal Title

      Current Therapy

      Volume: 39 Pages: 837-843

    • Related Report
      2021 Research-status Report
  • [Presentation] The circulating furin-cleaved/mature PCSK9 ratio as a novel prognositc marker in statin-naive patients with acute ST elevation myocardial infarction2022

    • Author(s)
      Sawaguchi J, Saeki Y, Oda M, Takamura TA, Fujibayashi K, Wakasa M, Akao H, Kitayama M, Kawai Y, Kajinami K.
    • Organizer
      The 86th annual scientific meeting of the Japanese Circulation Society
    • Related Report
      2022 Research-status Report
  • [Presentation] ABCC6遺伝子に片親性イドダイソミーによるナンセンス変異ホモ接合を認めた弾性線維性仮性黄色腫症の1例2021

    • Author(s)
      秋田恵理、小田美奈子、高村敬明、若狭 稔、赤尾浩慶、河合康幸、梶波康二
    • Organizer
      第53回日本動脈硬化学会総会・学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] Patient-centered, a multidisciplinary team-based approach in ASCVD prevention: new direction2021

    • Author(s)
      Kouji Kajinami
    • Organizer
      The 18th International Symposium on Atherosclerosis
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] 循環器疾患最新の治療2024-2025(伊藤浩、山下武志編) 動脈硬化のスクリーニング2023

    • Author(s)
      田中 海、梶波康二
    • Total Pages
      576
    • Publisher
      南江堂
    • ISBN
      9784524204427
    • Related Report
      2023 Research-status Report
  • [Book] 循環器疾患最新の治療2022-2023(伊藤 浩、山下武志編) 脂質異常症(高脂血症)2022

    • Author(s)
      澤口潤、梶波康二
    • Total Pages
      520
    • Publisher
      南江堂
    • ISBN
      9784524229956
    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2024-12-25  

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