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Regulatory mechanisms of PSMA expression and its molecular functions in prostate cancer-associated vascular endothelial cells.

Research Project

Project/Area Number 21K09427
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56030:Urology-related
Research InstitutionEhime University

Principal Investigator

Miura Noriyoshi  愛媛大学, 医学部附属病院, 講師 (80554427)

Co-Investigator(Kenkyū-buntansha) 雑賀 隆史  愛媛大学, 医学系研究科, 教授 (10314676)
東山 繁樹  愛媛大学, プロテオサイエンスセンター, 教授 (60202272)
菊川 忠彦  愛媛大学, 医学部附属病院, 准教授 (70444734)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords前立腺癌 / PSMA / 血管内皮細胞
Outline of Research at the Start

前立腺特異的膜抗原 (prostate specific membrane antigen; PSMA)は前立腺癌マーカーとしては確立されているものの、その分子機能や前立腺癌組織形成における機能は殆ど不明である。本研究では、質量分析を基盤技術として、前立腺癌細胞由来microvesicle(MV)による正常血管内皮細胞でのPSMA発現誘導機構とPSMA陽性血管内皮細胞の質的変化を解明することで前立腺癌増殖の新しい知見を得る。

Outline of Final Research Achievements

We found that the addition of a 10,000 g pellet fraction of the culture supernatant of a prostate cancer cell line, LNCaP cells, to the medium of normal vascular endothelial cells, human umbilical vein endothelial cells (HUVECs), induces PSMA transcription. Furthermore, we found that microvesicles secreted by prostate cancer cells induce PSMA expression in surrounding vascular endothelial cells and enhance their angiogenic potential. In addition, we found that only tumor vessels around renal carcinoma were positive for PSMA, suggesting that PSMA may also play an important role in tumor angiogenesis in solid tumors other than prostate cancer.

Academic Significance and Societal Importance of the Research Achievements

前立腺特異的膜抗原 (prostate specific membrane antigen; PSMA)は前立腺癌マーカーとしては確立されているものの、その分子機能や前立腺癌組織形成における機能は殆ど不明である。本研究では、前立腺癌細胞由来microvesicle(MV)による正常血管内皮細胞でのPSMA発現誘導機構とPSMA陽性血管内皮細胞の質的変化を解明することで前立腺癌増殖の新しい知見を得ることができた。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (2 results)

All 2024 2021

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] PSMA‐positive membranes secreted from prostate cancer cells have potency to transform vascular endothelial cells into an angiogenic state2021

    • Author(s)
      Watanabe Ryuta、Maekawa Masashi、Kiyoi Takeshi、Kurata Mie、Miura Noriyoshi、Kikugawa Tadahiko、Higashiyama Shigeki、Saika Takashi
    • Journal Title

      The Prostate

      Volume: 81 Issue: 16 Pages: 1390-1401

    • DOI

      10.1002/pros.24237

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Prostate-specific membrane antigen expression in endothelial cells of renal tumor-associated neovasculature and risk of recurrence for renal cell carcinoma2024

    • Author(s)
      Tadahiko Kikugawa
    • Organizer
      JUA
    • Related Report
      2023 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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