• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Investigation for involvement of MMP-2 in bone healing with imaging of proteome and mineralome

Research Project

Project/Area Number 21K09825
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57010:Oral biological science-related
Research InstitutionTohoku University

Principal Investigator

Sasano Yasuyuki  東北大学, 歯学研究科, 教授 (30196191)

Co-Investigator(Kenkyū-buntansha) Yang MuChen  東北大学, 歯学研究科, 助教 (50886447)
鈴木 治  東北大学, 歯学研究科, 教授 (60374948)
矢尾 育子  関西学院大学, 生命環境学部, 教授 (60399681)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsMMP-2欠損マウス / 脛骨 / 骨密度 / マイクロX線CT / エネルギー分散型X線分析 / 破骨細胞 / 下顎頭 / 骨髄腔 / 分析走査電子顕微鏡 / 骨 / 石灰化 / 規格化骨欠損 / 質量顕微鏡
Outline of Research at the Start

骨の発生過程で、MMP-2による基質タンパクの分解は石灰化、すなわちミネラル結晶の沈着と成長を進行させ、骨基質を成熟させる。一方、骨の再生過程については知見が乏しい。本研究では、MMP-2欠損マウスを用いて、再生骨のタンパク構成すなわちプロテオームを質量顕微鏡で、ミネラル構成すなわちミネラロームを分析走査電子顕微鏡で、それぞれ可視化し、MMP-2欠損マウスと野生型マウスにおける再生骨の石灰化と成熟を検討することを目的とする。この際、MMP-2欠損マウスの再生骨で分解されずに残存し、野生型マウスで分解される基質タンパクを画像上で選択して同定し、石灰化における基質タンパクの役割を解析する。

Outline of Final Research Achievements

he present study was designed to compare calcification and histological characteristics of tibiae between the mice devoid of matrix metalloproteinase (MMP)-2 (KO) and the wild-type mice (WT). Calcification was examined using the micro-computed tomography (micro-CT) for the bone mineral density (BMD) and the scanning electron microscope equipped with an energy dispersive X-ray spectroscope (SEM-EDX) for analysis of elements that constitute the calcium phosphate mineral. Histological sections were cut and stained with hematoxylin and eosin or alcian blue. The BMD of the cortical bone was lower in KO whereas element concentrations and distributions were not different between KO and WT. Histology showed that the cortical bone is thinner and its surface facing the bone marrow is more irregular in KO. The present study suggested that MMP-2 has some role in bone calcification and histological organization.

Academic Significance and Societal Importance of the Research Achievements

MMP-2が骨の石灰化に役割を担うことは想定されてきたが、ミネラルにおける元素の構成や組織構造への影響は不明である。本研究ではMMP-2遺伝子欠損マウスで生後7週齢及び25週齢の段階の骨密度が低下する傾向を確認した一方で、カルシウムとリンの濃度には野生型マウスと違いは見られなかった。組織学的検討では、MMP-2遺伝子欠損マウスで皮質骨が薄く、また、骨髄腔に面する骨面の凹凸が顕著で、破骨細胞の関与が示唆された。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (21 results)

All 2023 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 9 results,  Open Access: 5 results) Presentation (10 results) (of which Invited: 3 results)

  • [Int'l Joint Research] Sichuan University/Shenzhen University General Hospital(中国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] Fujian Medical University(中国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Visualization of the localization of phospholipids in developing rat teeth by matrix-assisted laser desorption/ionization imaging mass spectrometry2023

    • Author(s)
      Sasano Y, Konno A, Nakamura M, Henmi A, Mayanagi M, Yang MC, Yao I
    • Journal Title

      Biomedical Research

      Volume: 44 Issue: 4 Pages: 173-179

    • DOI

      10.2220/biomedres.44.173

    • ISSN
      0388-6107, 1880-313X
    • Year and Date
      2023-08-03
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immunohistochemical characterization of the mandibular condyle for type I and II collagen, aggrecan, MMP-9, and MMP-13 in MMP-2-deficient mice2023

    • Author(s)
      Yang MC, Nakamura M, Mayanagi M, Sasano Y
    • Journal Title

      Biomedical Research

      Volume: 44 Issue: 2 Pages: 65-72

    • DOI

      10.2220/biomedres.44.65

    • ISSN
      0388-6107, 1880-313X
    • Year and Date
      2023-04-02
    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] MMP-2遺伝子欠損マウスの脛骨における組織構造と石灰化に関する検討2023

    • Author(s)
      庄子昌利、Mu Chen Yang、真栁みゆき、萱場敦子、笹野泰之
    • Journal Title

      東北大学歯学雑誌

      Volume: 41・42 Pages: 21-26

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age-Related Gene and Protein Expression in Mouse Mandibular Condyle Analyzed by Cap Analysis of Gene Expression and Immunohistochemistry2023

    • Author(s)
      Yang Mu-Chen、Nakamura Megumi、Kageyama Yoko、Igari Yohei、Sasano Yasuyuki
    • Journal Title

      Gerontology

      Volume: 69 Issue: 11 Pages: 1295-1306

    • DOI

      10.1159/000533921

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The visualization of the mineral and protein distribution in the same histological sections of rat calcified growth plate cartilage2022

    • Author(s)
      Mayanagi Miyuki、Nakamura Megumi、Henmi Akiko、Sasano Yasuyuki
    • Journal Title

      Journal of Oral Biosciences

      Volume: 64 Issue: 4 Pages: 461-464

    • DOI

      10.1016/j.job.2022.08.005

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of matrix metalloproteinase‐3 and ‐10 is up‐regulated in the periodontal tissues of aged mice2022

    • Author(s)
      Kageyama Yoko、Nakamura Megumi、Igari Yohei、Yamaguchi Satoshi、Oguchi Akiko、Murakawa Yasuhiro、Hattori Yoshinori、Sasano Yasuyuki
    • Journal Title

      Journal of Periodontal Research

      Volume: - Issue: 4 Pages: 733-741

    • DOI

      10.1111/jre.12996

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Calcification and resorption of mouse Meckel’s cartilage analyzed by von Kossa and tartrate-resistant acid phosphatase histochemistry and scanning electron microscopy/energy-dispersive X-ray spectrometry2021

    • Author(s)
      Nakamura Megumi、Yang Mu-Chen、Ashida Keijyu、Mayanagi Miyuki、Sasano Yasuyuki
    • Journal Title

      Anatomical Science International

      Volume: 97 Issue: 2 Pages: 213-220

    • DOI

      10.1007/s12565-021-00643-6

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Three-dimensional visualization of osteoclasts in embryonic mouse mandibles using SEM array tomography2021

    • Author(s)
      Caiyu Liao、Nakamura Megumi、Mayanagi Miyuki、Kayaba Atsuko、Sasano Yasuyuki
    • Journal Title

      Journal of Oral Biosciences

      Volume: 63 Issue: 4 Pages: 401-407

    • DOI

      10.1016/j.job.2021.10.003

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Application of cryopreservation to tooth germ transplantation for root development and tooth eruption2021

    • Author(s)
      Li Xinghan、Nakamura Megumi、Tian Weidong、Sasano Yasuyuki
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1

    • DOI

      10.1038/s41598-021-88975-1

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Immunohistochemical localization of MMP-9, MMP-13, and extracellular matrix proteins in the mandibular condyle of MMP-2-deficient mice.2023

    • Author(s)
      Mu Chen Yang, Megumi Nakamura, Yasuyuki Sasano
    • Organizer
      第65回歯科基礎医学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] マウス実験的歯周炎におけるMMP9とMMP13の発現に関する検討2023

    • Author(s)
      菅野千春, 中村恵, 大方広志, 山本寿則, 笹野泰之
    • Organizer
      第66回秋季日本歯周病学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] マウス実験的歯周炎における歯槽骨吸収に対する加齢と炎症の影響2023

    • Author(s)
      中村 恵、Yang Mu-Chen、笹野 泰之
    • Organizer
      第65回歯科基礎医学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] マウス実験的歯周炎モデルを用いた加齢と炎症が及ぼす歯槽骨吸収への影響2023

    • Author(s)
      中村恵, Mu-Chen Yang, 山口哲史, 大方広志, 笹野泰之
    • Organizer
      第66回秋季日本歯周病学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 下顎骨発生における破骨細胞 ・ 破軟骨細胞の役割と意義2023

    • Author(s)
      中村恵、Mu-Chen Yang、笹野泰之
    • Organizer
      第128回日本解剖学会総会・全国学術集会 シンポジウム 「骨発生・成長における破骨細胞と周囲の細胞群の連携機構」
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Whole transcriptome analysis with CAGE revealed that MMPs-3, -9, and -13 were differentially expressed in the mandibular condyle of aged mice.2023

    • Author(s)
      Mu-Chen Yang, Megumi Nakamura, Yasuyuki Sasano
    • Organizer
      第128回日本解剖学会総会・全国学術集会
    • Related Report
      2022 Research-status Report
  • [Presentation] 副甲状腺ホルモン投与による骨芽細胞の基質石灰化と骨細胞分化の組織化学的解析2023

    • Author(s)
      北構-原口真衣、 長谷川智香、 網塚憲生、 笹野泰之
    • Organizer
      第128回日本解剖学会総会・全国学術集会
    • Related Report
      2022 Research-status Report
  • [Presentation] 硬組織の発生と修復の過程における石灰化進行機構2022

    • Author(s)
      笹野泰之
    • Organizer
      明海大学 歯学部 第412回研修会・第282回大学院FD研修会 (総合セミナー)
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 下顎の発生課程において石灰化組織吸収に寄与する細胞2022

    • Author(s)
      中村恵、Mu-Chen Yang、笹野 泰之
    • Organizer
      第64回歯科基礎医学会学術大会 アップデートシンポジウム 「骨形成の場を整える吸収系細胞の検討」
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] CAGE解析を用いたマウス歯周組織における老化関連細胞外マトリックス分解酵素の探索2021

    • Author(s)
      中村恵、笹野泰之
    • Organizer
      日本解剖学会 第67回東北・北海道連合支部学術集会
    • Related Report
      2021 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi