| Project/Area Number |
21K09998
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
加来 賢 新潟大学, 医歯学系, 准教授 (30547542)
魚島 勝美 新潟大学, 医歯学系, 教授 (50213400)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | trans-omics / extracellular matrix / bone phenotype / bone regeneration |
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| Outline of Research at the Start |
1.Identification of the molecular cues that distinguish the bone type
2.Analysis of ECM protein profile that distinguish the bone type
3.Integration of the trans-omics data and construction of the molecular network
4.Animal experiment, induction of trabecular bone towards cortical bone.
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| Outline of Final Research Achievements |
Bone augmentation involves a complex biological process where the extracellular matrix (ECM) plays a critical role in the structure and function of bone. However, the method used for experimentation significantly affects the profile of ECM proteins, so there is a need for better protein extraction techniques to accurately identify bone profiles. It is essential to optimize protein extraction methods to maintain the natural state of ECM proteins for precise analysis. Our data indicates that the composition of ECM changes during early osteoblast differentiation, showing an increase in Matrisome proteins. These findings suggest a dynamic shift in ECM composition, indicating its evolving role in supporting and regulating the osteogenic process.
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| Academic Significance and Societal Importance of the Research Achievements |
Bone augmentation is a complex process with unclear mechanisms. The ECM is vital for bone structure and cellular functions, impacted by collagen crosslinks. Improved protein extraction methods are needed to study ECM profiles in bone accurately.
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