Elucidating transmission dynamics for soil-transmitted helminths based on environmental DNA analysis
Project/Area Number |
21K12269
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Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 63040:Environmental impact assessment-related
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Research Institution | Dokkyo Medical University |
Principal Investigator |
オオタケサトウ マルセロ 獨協医科大学, 医学部, 助教 (50771896)
|
Co-Investigator(Kenkyū-buntansha) |
迫 康仁 旭川医科大学, 医学部, 教授 (40312459)
サトウ 恵 新潟大学, 医歯学系, 准教授 (70601813)
|
Project Period (FY) |
2021-04-01 – 2024-03-31
|
Project Status |
Granted (Fiscal Year 2022)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
Keywords | Ecohealth / One-health / environmental DNA / STH / qPCR / STHs / prophylaxis |
Outline of Research at the Start |
土壌伝播蠕虫の感染者は世界で約15億人もおり、その感染が患者の生活の質を下げ、流行地域の経済的発展と安定を妨げているが、十分な対策が立てられていない。そのため、ヒトの健康を守るための「感染症の効果的な予防法」を確立することは非常に重要である。本研究の目的は、エコヘルスの概念に基づき、環境要素からの土壌伝播蠕虫のDNA検出法を環境DNA手法に応用して開発し、流行地域における感染リスクの評価法を確立し、予防対策に資するデータを提示することである。本研究で得られる成果は、多くの病原体の環境中でのダイナミクスの解明と、それを基盤とした予防対策の確立に容易に応用でき、社会に与えるインパクトは大きい。
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Outline of Annual Research Achievements |
Goal: Improvement of soil transmitted helminths-specific DNA detection method. The analyses of DNA sequences of the parasite species causing STH retrieved from the database allowed us to develop suitable probes for STH detection. We designed the environmental DNA detection assay for soil samples, using mitochondrial COX1 gene. The specific sets of primers and probes were designed within an expected short length of the amplified DNA (about 100 to 200 bp). Tests using DNA extracted from adult worms (target and non-target) showed the assays are suitable with a high specificity. However, it needs improvement on the sensitivity, for detection of small quantities of target DNA usually found eDNA. We are now working on a new technique based in multi-probe detection which increases the sensitivity of the assay without loss in specificity (as reported by our group in detection of Opisthorchis viverrini in eDNA from water samples).
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Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The project is progressing smoothly at the laboratory, however we still are experiencing delay in field samples. Regarding the developed assays, we think we can increase the sensitivity of the technique using a multi-probe system, and we are on the way to standardize and test the assay using soil samples.
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Strategy for Future Research Activity |
We are looking forward to standardize the multi-probe detection assay and apply it to eDNA extracted from soil samples from the field.
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Report
(2 results)
Research Products
(2 results)