| Project/Area Number |
21K12269
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 63040:Environmental impact assessment-related
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| Research Institution | Niigata University of Pharmacy and Medical and Life Sciences (2023)
Dokkyo Medical University (2021-2022) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
迫 康仁 旭川医科大学, 医学部, 教授 (40312459)
サトウ 恵 新潟大学, 医歯学系, 准教授 (70601813)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ecohealth / one-health / environmental DNA / helminths / pPCR / STH / Ecohealth / One-health / Helminths / qPCR / STHs / prophylaxis |
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| Outline of Research at the Start |
土壌伝播蠕虫の感染者は世界で約15億人もおり、その感染が患者の生活の質を下げ、流行地域の経済的発展と安定を妨げているが、十分な対策が立てられていない。そのため、ヒトの健康を守るための「感染症の効果的な予防法」を確立することは非常に重要である。本研究の目的は、エコヘルスの概念に基づき、環境要素からの土壌伝播蠕虫のDNA検出法を環境DNA手法に応用して開発し、流行地域における感染リスクの評価法を確立し、予防対策に資するデータを提示することである。本研究で得られる成果は、多くの病原体の環境中でのダイナミクスの解明と、それを基盤とした予防対策の確立に容易に応用でき、社会に与えるインパクトは大きい。
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| Outline of Final Research Achievements |
In this study, the STH to be detected were A lumbricoides, T trichiura and Hookworms (A duodenale, and N americanus). From the aligned sequences of cox1 gene by species of parasite we determine specific variable/conserved areas and design sets of primers/probes to be used in multiplex qPCR. The assay was screened in silico for specificity. Worms' DNA were used to attest the usefulness of the system. Then, soil samples from endemic area were used. The soil eDNA was extracted and tested, completing the goals of this research project with the correlation of positive STH cases with environmental contamination. Furthermore, we are still analyzing more soil samples to increase the number of tests, and use it to design an accurate risk mapping of STH infection sites from endemic areas.
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| Academic Significance and Societal Importance of the Research Achievements |
The results showed one sample from a house surrounding where the residents presented positive result for Ascaris lumbricoides (presenting a relationship on environment and human parasitic status). This is the first study showing the usefulness of eNA in soils samples for determination of STH.
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