| Project/Area Number |
21K12665
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Optical Imaging / Fluorescence Microscopy / Brain Imaging / optical imaging / fluorescence microscopy / 3d brain imaging / neuroinformatics / brain imaging / fluorescence microscope / 3D imaging |
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| Outline of Research at the Start |
The research focus is to:
1. Establish 3-dimensional microscopy using novel deep ultraviolet fluorescence microscopy for volumetric analysis of brain structures in normal and diseased models
2. Develop open microscopy and open data toolkit to be put in the public domain for general purpose use.
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| Outline of Final Research Achievements |
Fluorescence microscopy is an important tool in neuroscience which enables imaging across different spatial and temporal scales to obtain the structural and functional organizational details of the brain. The cost and complex design of microscopes that can do whole brain imaging significantly affect the use of microscopy tool for scientific discoveries. This research project focused on developing and establishing a simple and cost-effective fluorescence microscopy for 3-dimensional brain imaging. Deep ultraviolet illumination was utilized which allows for simplified optical design and allows for optical sectioning on block-face imaging. A combination of optical sectioning and mechanical sectioning technique was utilized using open-source tools for whole brain imaging using conventional fluorescent dyes. Adopting and adapting open source solutions (hardware and software) for this microscopy allows for easy duplication of this tool by other labs.
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| Academic Significance and Societal Importance of the Research Achievements |
We developed and established a simple and cost-effective imaging tool (fluorescence microscopy set-up) for whole brain and volumetric imaging using novel light source - deep ultraviolet illumination. Open source tools were adopted and developed for data acquisition and image analysis.
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