A common and potentially prebiotic activating agent for phosphorylation of nucleosides and oligomerization of nucleotides and amino acids
Project/Area Number |
21K14029
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 17050:Biogeosciences-related
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Research Institution | Tokyo Institute of Technology |
Principal Investigator |
蟻 瑞欽 東京工業大学, 地球生命研究所, 研究員 (00860090)
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Project Period (FY) |
2021-04-01 – 2024-03-31
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Project Status |
Granted (Fiscal Year 2022)
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Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | amino acids / activating chemistry / peptide synthesis / RNA polymerization / solid-phase synthesis / activation chemistry / phosphorylation / polymerization |
Outline of Research at the Start |
Recently, we proposed a “continuous reaction networks” model, and demonstrated that a wide variety of key precursors, useful for the synthesis of RNA monomers and amino acids, are constantly formed. The remaining key question of this model is whether this continuous reaction model can further enable the subsequent step towards RNA polymers generation compatible with forming other important substrates, such as oligopeptides. Therefore, this study aims for understanding how to generate RNA monomers, amino acids, as well as their polymers in a continuous manner under a single-pot environment.
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Outline of Annual Research Achievements |
The proposed cyanogen chloride induced pathway for the synthesis of RNA and peptides includes: 1. phosphorylation of nucleosides to nucleotides; 2. activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization;3. activation of amino acids to peptides and deprotection. This year, the feasibility of cyanogen chloride activated polymerization of amino acids in the solid-phase peptide synthesis has been demonstrated. We also observed that activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization and amino acids polymerization can occur in a one-pot synthesis using cyanogen chloride as a common activating agent.
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
This project was divided into two stages. Stage 1 is to explore cyanogen chloride activated phosphorylation of nucleosides to nucleotides, and stage 2 is to assess cyanogen chloride (ClCN) activated polymerization of amino acids to peptides. This project was scheduled to be finished in two years. In the original plan, stage 1 would be finished last year. Since I already had some primary result of stage 2, so I changed the sequence of the project plan and continued to explore the feasibility of stage 2 and finished the goals of stage 2.
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Strategy for Future Research Activity |
All the experiments in the stage 2 have been finished. I am writing the paper now to summary the whole results in the stage 2. I will continue to explore the possibility of stage 1 this year.
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Report
(2 results)
Research Products
(4 results)