| Project/Area Number |
21K14029
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 17050:Biogeosciences-related
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
Yi Ruiqin 東京工業大学, 地球生命研究所, 研究員 (00860090)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | The Origins of Life / Amino acids / RNA / Peptides / Polymerzation / Activating Chemistry / amino acid / peptides / polymerization / chemical activation / nucleotides / amino acids / activating chemistry / peptide synthesis / RNA polymerization / solid-phase synthesis / activation chemistry / phosphorylation |
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| Outline of Research at the Start |
Recently, we proposed a “continuous reaction networks” model, and demonstrated that a wide variety of key precursors, useful for the synthesis of RNA monomers and amino acids, are constantly formed. The remaining key question of this model is whether this continuous reaction model can further enable the subsequent step towards RNA polymers generation compatible with forming other important substrates, such as oligopeptides. Therefore, this study aims for understanding how to generate RNA monomers, amino acids, as well as their polymers in a continuous manner under a single-pot environment.
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| Outline of Final Research Achievements |
cyanogen chloride was proposed to activate the synthesis of RNA and peptides. In this research, the activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization and activation of amino acids to peptides has been demonstrated. The cyanogen chloride activated polymerization of amino acids in the solid-phase peptide synthesis was also demonstrated, which could be used for the peptide synthesis in the industry. The activation of nucleotides to their 5′-phosphorimidazoles for RNA polymerization and amino acids polymerization has been found to occur in a one-pot synthesis using cyanogen chloride as a common activating agent. These results suggest that cyanogen chloride is a very useful activating reagent in the prebiotic chemistry.
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| Academic Significance and Societal Importance of the Research Achievements |
This study provides an understanding how RNA and peptides could evolve chemically starting from simple molecules to complex polymers under a one-single environment. The performed peptides synthesis affords an idea of the extension of the outcome from prebiotic chemistry to appliable chemistry.
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