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Microbial influence on surface microstructural changes of uranium dioxide in aqueous environments

Research Project

Project/Area Number 21K14568
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 31010:Nuclear engineering-related
Research InstitutionJapan Atomic Energy Agency

Principal Investigator

Kitagaki Toru  国立研究開発法人日本原子力研究開発機構, 福島廃炉安全工学研究所 廃炉環境国際共同研究センター, 主任研究員 (30770036)

Project Period (FY) 2021-04-01 – 2025-03-31
Project Status Completed (Fiscal Year 2024)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords微生物 / 放射線 / 福島第一原子力発電所 / 二酸化ウラン / 溶解 / 燃料デブリ / 遺伝子 / ゲノム / 変異 / ウラン / チェルノブイリ / 液中その場観察 / 細菌 / 脱窒菌 / 原子間力顕微鏡 / ジルコン / 鉄酸化細菌 / マンガン酸化細菌
Outline of Research at the Start

核燃料物質の処分等の観点から、水中の二酸化ウランの溶解挙動を評価する実験が数多く行われているが、微細な表面構造の変化を評価していないため、実験毎にバラついた結果しか得られていない。また、微生物による影響を考慮していないこと等による、実環境下での溶解挙動との乖離が課題となっている。
本研究では、各種溶液中での二酸化ウランの微細な表面構造の変化や、微生物との相互作用をナノスケールから液中その場観察すると同時に、局所的な化学状態の変化を同定することにより、微生物を含む液中の二酸化ウランのナノスケールの表面構造や化学状態の変化を詳細に把握する。

Outline of Final Research Achievements

The objective of this study is to evaluate the microbially enhanced dissolution of uranium dioxide. To this end, it is essential to isolate and characterize microorganisms from environments rich in uranium dioxide.
In this study, stagnant water was sampled from Unit 2 of the Fukushima Daiichi Nuclear Power Plant nine years after the accident. Bacterial cultures were successfully obtained from this sample, and multiple strains were isolated. DNA was extracted from these strains and analyzed using next-generation sequencing. The sequences were assembled to determine the complete genome of each strain. By comparing the genomes with reference databases, the bacterial species were identified. However, partial discrepancies were observed between the isolated strains and the reference genomes, suggesting that DNA mutations may have occurred under high-radiation conditions, potentially leading to the acquisition of novel traits.

Academic Significance and Societal Importance of the Research Achievements

本研究は、福島第一原発事故後の高放射線環境から微生物を分離・解析し、二酸化ウラン溶解への影響を評価したものである。得られた菌株のゲノム配列には基準株と異なる変異が認められ、放射線下での進化や新たな特性獲得の可能性が示唆された。これにより、極限環境における微生物進化の理解が進むとともに、微生物による核燃料の溶解機構解明に貢献する。さらに、放射性廃棄物管理や環境修復技術への応用が期待され、原子力災害後の生態系評価にも資する社会的意義を有する。

Report

(5 results)
  • 2024 Annual Research Report   Final Research Report ( PDF )
  • 2023 Research-status Report
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 2 results)

  • [Int'l Joint Research] ISP-NPP(ウクライナ)

    • Related Report
      2022 Research-status Report
  • [Journal Article] Microbiome analysis of the restricted bacteria in radioactive element-containing water at the Fukushima Daiichi Nuclear Power Station2024

    • Author(s)
      Warashina Tomoro、Sato Asako、Hinai Hiroshi、Shaikhutdinov Nurislam、Shagimardanova Elena、Mori Hiroshi、Tamaki Satoshi、Saito Motofumi ... &、Kanai Akio
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 90 Issue: 4

    • DOI

      10.1128/aem.02113-23

    • Related Report
      2024 Annual Research Report 2023 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Degradation of nuclear fuel debris analog by siderophore-releasing microorganisms2023

    • Author(s)
      Ohnuki Toshihiko、Nakase Masahiko、Liu Jiang、Dotsuta Yuma、Satou Yukihiko、Kitagaki Toru、Kozai Naofumi
    • Journal Title

      Journal of Nuclear Science and Technology

      Volume: 61 Issue: 3 Pages: 384-396

    • DOI

      10.1080/00223131.2023.2231951

    • Related Report
      2023 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aging of fuel-containing materials (fuel debris) in the Chornobyl (Chernobyl) Nuclear Power Plant and its implication for the decommissioning of the Fukushima Daiichi Nuclear Power Station2023

    • Author(s)
      Kitagaki Toru、Krasnov Viktor A.、Ikeda-Ohno Atsushi
    • Journal Title

      Journal of Nuclear Materials

      Volume: 576 Pages: 154224-154224

    • DOI

      10.1016/j.jnucmat.2022.154224

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Alteration of fuel debris simulants by Bacillus subtilis2023

    • Author(s)
      Liu Jiang、Dotsuta Yuma、Kitagaki Toru、Aoyagi Noboru、Mei Huiyang、Takano Masahide、Kozai Naofumi
    • Journal Title

      Journal of Nuclear Science and Technology

      Volume: - Issue: 8 Pages: 1-11

    • DOI

      10.1080/00223131.2022.2162995

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Potential bacterial alteration of nuclear fuel debris: a preliminary study using simulants in powder and pellet forms2022

    • Author(s)
      Liu Jiang、Dotsuta Yuma、Sumita Takehiro、Kitagaki Toru、Ohnuki Toshihiko、Kozai Naofumi
    • Journal Title

      Journal of Radioanalytical and Nuclear Chemistry

      Volume: 331 Issue: 6 Pages: 2785-2794

    • DOI

      10.1007/s10967-022-08324-y

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dissolution and precipitation behaviors of zircon under the atmospheric environment2022

    • Author(s)
      Kitagaki Toru、Yoshida Kenta、Liu Pengfei、Shobu Takahisa
    • Journal Title

      npj Materials Degradation

      Volume: 6 Issue: 1 Pages: 13-13

    • DOI

      10.1038/s41529-021-00214-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of the dissolution behavior of zircon using high-resolution phase-shift interferometry microscope2021

    • Author(s)
      KITAGAKI Toru
    • Journal Title

      Journal of Nuclear Materials

      Volume: 557 Pages: 153254-153254

    • DOI

      10.1016/j.jnucmat.2021.153254

    • Related Report
      2021 Research-status Report
    • Peer Reviewed

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Published: 2021-04-28   Modified: 2026-01-16  

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