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Probe-type nanowire sensor for label-free, in-situ, ultrasensitive detection of biomarkers from single cell in adherent culture

Research Project

Project/Area Number 21K14653
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 34020:Analytical chemistry-related
Research InstitutionTokyo Metropolitan University

Principal Investigator

毛 思鋒  東京都立大学, 都市環境科学研究科, 助教 (40885315)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Granted (Fiscal Year 2021)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsChemical pen / Nanowire / Biosensing / Biomarker / Single cell / Microfluidics / nanowire / biosensor / Cell research / biomarker
Outline of Research at the Start

In this proposal, we aim to develop probe-type nanowire sensor for label free, in-situ, ultrasensitive detection of biomarkers from single cell in adherent culture. It might be a general strategy for molecule detection if we functionalized the nanowire with different types of receptors.

Outline of Annual Research Achievements

Our research group has developed a micro drawing device, the chemical pen, which enables to control solution in local area on surfaces at the nanoscale using confined open-space laminar flow. We have developed an open-space laminar flow approach for fabricating a single gold nanowire at precise position under normal condition. This method enabled us to fabricate gold nanowires with the widths ranged of 200 nm to several ten micrometers and lengths ranged of 3 mm or less at targeted locations. The gold nanowires were composed of multiple nanoparticles aggregated into a wire-like structure. The fabricated gold nanowire was demonstrated an excellent biosensing for IgA with an extremely low limit of detection (1 aM). Thus, this novel technique is expected to be applied in a wide range of fields. It is the first time to fabricate gold nanowires at precise position and in normal environment. Compare to Ag nanowire, gold nanowire is more widely used for biosensing. The high-performance IgA detection was achieved using a single gold nanowire in this study, however, by changing the modification of the gold nanowires according to the target substance, we expect to develop a sensor that can measure various substances with high sensitivity in the field of pharmacology, medical care, food analysis, environmental analysis and even of infection diagnose.

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

we present an open-space laminar flow approach for fabricating single gold nanowire in a regioselective manner for the first time. This method enabled us to fabricate gold nanowires with the widths ranged of 200 nm to several ten micrometers and lengths ranged of 3 mm or less at targeted locations. The gold nanowires were composed of multiple nanoparticles aggregated into a wire-like structure. Due to the structure, a highly sensitive biosensor was successfully realized as sensitive as 1 aM IgA using this single gold nanowire. The high-performance IgA detection was achieved using a single gold nanowire in this study.

Strategy for Future Research Activity

The setup of resistance recording system.
The detection system involves a conductive chamber, a nanowire sensor, an I/V converter, A/D converter and a computer for record. A preparing nanowire sensor and A/D transformer will be set in a conductive chamber for eliminating electromagnetic interference.
Detection of CEA from a single cell using the nanowire sensor.
Caco-2 cell are cultured in petridish with low cell density for use. Before detection of CEA, the culture medium in the petridish will be replaced with fresh culture medium. The nanowire sensor that have connected with resistance recording system will be manipulated close to the target cell. Then, the resistance change will be recorded by software in the computer.

Report

(1 results)
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (4 results) Patent(Industrial Property Rights) (2 results)

  • [Int'l Joint Research] Tsinghua University/College of Science/Department of Chemistry(中国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Regioselective fabrication of gold nanowires using open-space laminar flow for attomolar protein detection2022

    • Author(s)
      Nishitani Yuki、Kasai Nahoko、Nakajima Hizuru、Kato Shungo、Mao Sifeng、Uchiyama Katsumi
    • Journal Title

      Chemical Communications

      Volume: 58 Issue: 27 Pages: 4308-4311

    • DOI

      10.1039/d2cc00507g

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Push-pull system for single cell stimulation and real-time electrochemical detection of lactate response2022

    • Author(s)
      Sifeng Mao, Haifeng Lin, Nahoka Kasai, Shungo Kato, Hizuru Nakajima, Katsumi Uchiyama
    • Organizer
      2022年第69回 応用物理学会春季学術講演会, 2022/3/22-26オンライン開催,24p-D316-10
    • Related Report
      2021 Research-status Report
  • [Presentation] 化学ペンを用いた位置選択的微小金ワイヤの作製2021

    • Author(s)
      西谷祐貴,河西奈保子,中嶋秀,加藤俊吾,毛思鋒,(内山一美)
    • Organizer
      第82回応用物理学会秋季学術講演会
    • Related Report
      2021 Research-status Report
  • [Presentation] Fluid mechanism of three-nozzle chemical pen for nanofabrication2021

    • Author(s)
      Peng Chenhan, Kasai Nahoko, Nakajima Hizuru, Kato Shungo, Mao Sifeng, Katsumi Uchiyama
    • Organizer
      第82回応用物理学会秋季学術講演会,2021/9/21-9/23オンライン開催
    • Related Report
      2021 Research-status Report
  • [Presentation] Mechanism of micro-chemical pen for nanowire fabrications for biosensing2021

    • Author(s)
      Peng Chenhan, Kasai Nahoko, Nakajima Hizuru, Kato Shungo, Mao Sifeng, Katsumi Uchiyama
    • Organizer
      The 9th Chem-Bio Joint Seminar 2021
    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] 東京都公立大学法人2021

    • Inventor(s)
      内山一美,毛 思鋒,河西奈保子,林 海鋒,中嶋秀,加藤俊吾
    • Industrial Property Rights Holder
      内山一美,毛 思鋒,河西奈保子,林 海鋒,中嶋秀,加藤俊吾
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-137979
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] 東京都公立大学法人2021

    • Inventor(s)
      毛思鋒,河西奈保子,中嶋秀,加藤俊吾
    • Industrial Property Rights Holder
      毛思鋒,河西奈保子,中嶋秀,加藤俊吾
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-107375
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2022-12-28  

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