| Project/Area Number |
21K14653
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 34020:Analytical chemistry-related
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| Research Institution | Tokyo Metropolitan University |
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Principal Investigator |
毛 思鋒 東京都立大学, 都市環境科学研究科, 助教 (40885315)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2021)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Chemical pen / Nanowire / Biosensing / Biomarker / Single cell / Microfluidics / nanowire / biosensor / Cell research / biomarker |
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| Outline of Research at the Start |
In this proposal, we aim to develop probe-type nanowire sensor for label free, in-situ, ultrasensitive detection of biomarkers from single cell in adherent culture. It might be a general strategy for molecule detection if we functionalized the nanowire with different types of receptors.
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| Outline of Annual Research Achievements |
Our research group has developed a micro drawing device, the chemical pen, which enables to control solution in local area on surfaces at the nanoscale using confined open-space laminar flow. We have developed an open-space laminar flow approach for fabricating a single gold nanowire at precise position under normal condition. This method enabled us to fabricate gold nanowires with the widths ranged of 200 nm to several ten micrometers and lengths ranged of 3 mm or less at targeted locations. The gold nanowires were composed of multiple nanoparticles aggregated into a wire-like structure. The fabricated gold nanowire was demonstrated an excellent biosensing for IgA with an extremely low limit of detection (1 aM). Thus, this novel technique is expected to be applied in a wide range of fields. It is the first time to fabricate gold nanowires at precise position and in normal environment. Compare to Ag nanowire, gold nanowire is more widely used for biosensing. The high-performance IgA detection was achieved using a single gold nanowire in this study, however, by changing the modification of the gold nanowires according to the target substance, we expect to develop a sensor that can measure various substances with high sensitivity in the field of pharmacology, medical care, food analysis, environmental analysis and even of infection diagnose.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
we present an open-space laminar flow approach for fabricating single gold nanowire in a regioselective manner for the first time. This method enabled us to fabricate gold nanowires with the widths ranged of 200 nm to several ten micrometers and lengths ranged of 3 mm or less at targeted locations. The gold nanowires were composed of multiple nanoparticles aggregated into a wire-like structure. Due to the structure, a highly sensitive biosensor was successfully realized as sensitive as 1 aM IgA using this single gold nanowire. The high-performance IgA detection was achieved using a single gold nanowire in this study.
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| Strategy for Future Research Activity |
The setup of resistance recording system.
The detection system involves a conductive chamber, a nanowire sensor, an I/V converter, A/D converter and a computer for record. A preparing nanowire sensor and A/D transformer will be set in a conductive chamber for eliminating electromagnetic interference.
Detection of CEA from a single cell using the nanowire sensor.
Caco-2 cell are cultured in petridish with low cell density for use. Before detection of CEA, the culture medium in the petridish will be replaced with fresh culture medium. The nanowire sensor that have connected with resistance recording system will be manipulated close to the target cell. Then, the resistance change will be recorded by software in the computer.
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