| Project/Area Number |
21K14804
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
KUMRUNGSEE THANUTCHAPORN (カムランシー タナッチャポーン) 広島大学, 統合生命科学研究科(生), 准教授 (90781849)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2022)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Carnosine / Muscle regeneration / Skeletal muscle / Vitamin B6 / sarcopenia / Satellite cells / Twist2 |
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| Outline of Research at the Start |
For decades, carnosine has been believed to improve exercise performance and prevent sarcopenia, but its exact molecular mechanisms remain unknown. This study aims to clarify if carnosine has any effects on muscle satellite cells (SCs, Pax7+ cells) and recently identified muscle progenitor cells (PCs, Tw2+ cells), essential for skeletal muscle regeneration and maintaining muscle mass, respectively. Moreover, this study will determine how carnosine plays a role in muscle regeneration and muscle energy metabolism.
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| Outline of Annual Research Achievements |
In this year, we further conducted experiments to determine if a carnosine regulator, vitamin B6, has any effects on muscle regeneration. We found that vitamin B6 deficient (1 mg PN* HCl/kg diet) and supplemented (35 mg/kg diet) diets significantly decreased carnosine levels in skeletal muscles, as compared to vitamin B6 recommended level diet (7 mg/kg diet). Next, we found that vitamin B6 deficiency (1 mg/kg diet) impaired muscle regeneration, reflecting by lower regenerating muscle mass and smaller myofiber size than the recommended vitamin B6 level (7 mg/kg diet) diet. Surprisingly, a high dose of vitamin B6 (35 mg/kg diet) also worsened muscle regeneration, which was against our expectation. Taken together the results suggest that the proper intake levels of vitamin B6 and higher levels of muscle carnosine are important to ensure the proper process of muscle regeneration. PN:Pyridoxine Hydrochloride
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
More research collaborators have joined my group so that more work can be done. So, everything has gone smoothly.
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| Strategy for Future Research Activity |
In FY 2023, effects of vitamin B6 on satellite cell activity will be examine by applying a single myofiber technique. In addition, effects of vitamin B6 on muscle hypertrophy in aging mice will be determined.
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