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Roles of carnosine in skeletal muscle regeneration, and its applications

Research Project

Project/Area Number 21K14804
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38050:Food sciences-related
Research InstitutionHiroshima University

Principal Investigator

Kumrungsee Thanutchaporn  広島大学, 統合生命科学研究科(生), 准教授 (90781849)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordscarnosine / muscle regeneration / skeletal muscle / vitamin B6 / satellite cells / sarcopenia / muscle atrophy / Carnosine / Muscle regeneration / Skeletal muscle / Vitamin B6 / dexamethasone / Satellite cells / Twist2
Outline of Research at the Start

For decades, carnosine has been believed to improve exercise performance and prevent sarcopenia, but its exact molecular mechanisms remain unknown. This study aims to clarify if carnosine has any effects on muscle satellite cells (SCs, Pax7+ cells) and recently identified muscle progenitor cells (PCs, Tw2+ cells), essential for skeletal muscle regeneration and maintaining muscle mass, respectively. Moreover, this study will determine how carnosine plays a role in muscle regeneration and muscle energy metabolism.

Outline of Final Research Achievements

This study demonstrated that carnosine played a role in decreasing susceptibility of satellite cells (SCs) upon activation and in regulating SC proliferation. Carnosine was found to have less effects on the early stage of muscle regeneration, but play a role in maintaining muscle mass and fiber size during muscle growth or hypertrophy. Vitamin B6 (B6) was confirmed to be a carnosine regulator. B6 deficiency was found to markedly decrease carnosine and its substrate β-alanine levels in both mouse skeletal muscles and C2C12 muscle cells. B6 possibly played a role in maintaining quiescent SC pool and supporting proliferation and self-renewal of SCs. Both B6 deficiency and supplementation delayed muscle regeneration and suppressed muscle hypertrophy. However, B6 supplementation suppressed muscle atrophy by down-regulating MURF-1 and Atrogin-1 expressions, protein-degrading genes. The present study provides new insight into novel roles of carnosine and vitamin B6 in preventing sarcopenia.

Academic Significance and Societal Importance of the Research Achievements

This research is expected to make a significant contribution to society in terms of extending healthy life expectancy by providing the development of nutritional intervention for preventing sarcopenia, which is an extremely important issue for the aging society.

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (40 results)

All 2024 2023 2022 2021

All Journal Article (15 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 14 results,  Open Access: 9 results) Presentation (24 results) (of which Int'l Joint Research: 5 results,  Invited: 7 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Relationship of Low Vitamin B6 Status with Sarcopenia, Frailty, and Mortality: A Narrative Review2024

    • Author(s)
      Norihisa Kato*, Akiko Kimoto, Peipei Zhang, Chanikan Bumrungkit, Sajith Karunaratne, Noriyuki Yanaka, and Thanutchaporn Kumrungsee*
    • Journal Title

      Nutrients

      Volume: 16 Issue: 1 Pages: 177-177

    • DOI

      10.3390/nu16010177

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The modification of buckwheat polyphenols by different pretreatments and complexation, and its application in oat flour model2023

    • Author(s)
      Xinru Liu a 1, Lili Wang, Chaomin Li, Xi Li, Thanutchaporn Kumrungsee, Xuqing Zhai, Zhongkai Zhou, Ruge Cao
    • Journal Title

      Food Bioscience

      Volume: 56 Pages: 103133-103133

    • DOI

      10.1016/j.fbio.2023.103133

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of pressure cooking on phenolic compounds of quinoa2023

    • Author(s)
      Qianwei Ma a, Zhixiang Li, Thanutchaporn Kumrungsee, Wenda Huang, Ruge Cao
    • Journal Title

      Grain & Oil Science and Technology

      Volume: 6 Issue: 3 Pages: 127-134

    • DOI

      10.1016/j.gaost.2023.03.001

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Exogenous Penicillium camemberti Lipase Preparation Exerts Prebiotic-like Effects by Increasing Cecal Bifidobacterium and Lactobacillus Abundance in Rats2023

    • Author(s)
      Yongshou Yang, Huijuan Jia, Chika Ando, Hisanori Kato*, Thanutchaporn Kumrungsee*, Norihisa Kato, Akiko Kimoto, Shinji Fukuda, Manabu Kuroda, Kyoichi Nishio, and Shotaro Yamaguchi
    • Journal Title

      Fermentation

      Volume: 9 Issue: 3 Pages: 227-227

    • DOI

      10.3390/fermentation9030227

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effects of Processings and Complexation on Solubility, Antioxidant and Antibacterial Properties of Buckwheat Polyphenols2023

    • Author(s)
      Xinru Liu, Qianwei Ma, Yongjun Sun, Wenming Ju, Thanutchaporn Kumrungsee, Zhongkai Zhou, Lijuan Wang & Ruge Cao
    • Journal Title

      Food and Bioprocess Technology

      Volume: - Issue: 6 Pages: 1583-1595

    • DOI

      10.1007/s11947-023-03217-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Satellite cell content and muscle regeneration in a mouse model of NAFLD2022

    • Author(s)
      Saliu TP, Kumrungsee T, Mitsumoto K, Chen S, Yanaka N.
    • Journal Title

      Nutrition

      Volume: 96 Pages: 111570-111570

    • DOI

      10.1016/j.nut.2021.111570

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 高齢者のビタミン B6 栄養:フレイル,及びサルコペニアとの関連性2022

    • Author(s)
      Norihisa Kato*, Thanutchaporn Kumrungsee
    • Journal Title

      ビタミン

      Volume: 96 Pages: 325-327

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Aspergillus-Derived Cellulase Preparation Exhibits Prebiotic-like Effects on Gut Microbiota in Rats.2022

    • Author(s)
      Yang Y, *Kumrungsee T, Kato N, Fukuda S, Kuroda M, Yamaguchi S
    • Journal Title

      Fermentation

      Volume: 8 Issue: 2 Pages: 71-71

    • DOI

      10.3390/fermentation8020071

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Serum Amyloid A3 Promoter-Driven Luciferase Activity Enables Visualization of Diabetic Kidney Disease2022

    • Author(s)
      Saliu TP, Yazawa N, Hashimoto K, Miyata K, Kudo A, Horii M, Kamesawa M, Kumrungsee T, Yanaka N
    • Journal Title

      International journal of molecular sciences.

      Volume: 23 Issue: 2 Pages: 899-899

    • DOI

      10.3390/ijms23020899

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative study on molecular mechanism of diabetic myopathy in two different types of streptozotocin-induced diabetic models2022

    • Author(s)
      Saliu TP, *Kumrungsee T, Miyata K, Tominaga H, Yazawa N, Hashimoto K, Kamesawa M, Yanaka N
    • Journal Title

      Life Sciences

      Volume: 288 Pages: 120183-120183

    • DOI

      10.1016/j.lfs.2021.120183

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Emerging cardioprotective mechanisms of vitamin B6: a narrative review2021

    • Author(s)
      Kumrungsee Thanutchaporn、Peipei?Zhang、Yanaka Noriyuki、Suda Takashi、Kato Norihisa
    • Journal Title

      European Journal of Nutrition

      Volume: 61 Issue: 2 Pages: 605-613

    • DOI

      10.1007/s00394-021-02665-2

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Supplemental Aspergillus Lipase and Protease Preparations Display Powerful Bifidogenic Effects and Modulate the Gut Microbiota Community of Rats2021

    • Author(s)
      Yang Y, *Kumrungsee T, Kato N, Fukuda S, Kuroda M, Yamaguchi S
    • Journal Title

      Fermentation

      Volume: 7 Issue: 4 Pages: 294-294

    • DOI

      10.3390/fermentation7040294

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Satellite Cells Exhibit Decreased Numbers and Impaired Functions on Single Myofibers Isolated from Vitamin B6-Deficient Mice2021

    • Author(s)
      Komaru T, Yanaka N, *Kumrungsee T
    • Journal Title

      Nutrients

      Volume: 13 Issue: 12 Pages: 4531-4531

    • DOI

      10.3390/nu13124531

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rhabdomyolysis After Consumption of Freshwater Fish (Neolissochilus soroides)2021

    • Author(s)
      Chartkul M, Petvicharn KN, Kumrungsee T, Jiranantakan T, Chomchai S.
    • Journal Title

      Wilderness & Environmental Medicine

      Volume: 32 Issue: 3 Pages: 410-413

    • DOI

      10.1016/j.wem.2020.12.007

    • Related Report
      2021 Research-status Report
  • [Journal Article] Fermented date residue extract mix containing gamma-aminobutyric acid augments the immune function of mouse splenocytes2021

    • Author(s)
      Hattori K, Yamamoto Y, Fujii S, Kumrungsee T, Hasegawa M, Yoshida A, Suzuki T, Sambongi Y.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 85 Issue: 7 Pages: 1753-1758

    • DOI

      10.1093/bbb/zbab093

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Aspergillus-derived enzymes as a novel player in gut-brain axis modulation2023

    • Author(s)
      *Jason D. Braga, Yongshou Yang, Norihisa Kato, Thanutchaporn Kumrungsee
    • Organizer
      The 20th Anniversary Convention and Scientific Meeting of the Philippine Society for Probiotics and Functional Foods, Inc
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effects of vitamin B6 supplementation on muscle regeneration2023

    • Author(s)
      *小牟田陽香, 矢中規之, カムランシー タナッチャポーン
    • Organizer
      日本農芸化学会 2023年度中四国・西日本支部合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Blood GABA availability contributes to food intake suppression in mice2023

    • Author(s)
      *カムランシー タナッチャポーン, 長尾知香, 矢中規之
    • Organizer
      日本農芸化学会 2023年度中四国・西日本支部合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 食欲抑制栄養素による新規抗肥満治療法2023

    • Author(s)
      *Thanutchaporn Kumrungsee
    • Organizer
      広島大学若手研究者による研究シーズ発表会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Increased blood GABA induces a chronic food intake suppressant effect in mice2023

    • Author(s)
      *Thanutchaporn Kumrungsee, Tomoka Nagao, Noriyuki Yanaka
    • Organizer
      日本食品科学工学会 第70回記念大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Food science and technology for healthy food industries2023

    • Author(s)
      *Thanutchaporn Kumrungsee
    • Organizer
      Hybrid Seminar #2 Hiroshima University Alumni Association Indonesia (HUAA Indonesia)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 糖尿病マウスにおけるγ-アミノ酪酸摂取の骨格筋再生に及ぼす影響2023

    • Author(s)
      *堀井茉優, 矢中規之, Hawke Thomas, Kumrungsee Thanutchaporn
    • Organizer
      第77回日本栄養・食糧学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] メカニカルスティミュレイションによる骨格筋再生への影響2023

    • Author(s)
      *Bumrungkit Chanikan, Kumrungsee Thanutchaporn
    • Organizer
      第77回日本栄養・食糧学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 食餌性GABAおよびGABA分解阻害による新規抗肥満治療法2023

    • Author(s)
      *長尾知香, 矢中規之, Kumrungsee Thanutchaporn
    • Organizer
      第77回日本栄養・食糧学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 麹菌消化酵素のプレバイオティクス様作用2023

    • Author(s)
      *Thanutchaporn Kumrungsee, Yongshou Yang, Jason D. Braga, Norihisa Kato
    • Organizer
      第77回日本栄養・食糧学会大会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Plant and gut microbiota-derived protein metabolites and potential health functions2022

    • Author(s)
      ○Thanutchaporn Kumrungsee
    • Organizer
      2022 AOCS Annual Meeting & Expo
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effects of dietary GABA and its degrading inhibitor on appetite regulation2022

    • Author(s)
      ○Thanutchaporn Kumrungsee,長尾 知香,矢中 規之
    • Organizer
      日本農芸化学会中四国支部第62回講演会(例会)
    • Related Report
      2022 Research-status Report
  • [Presentation] ビタミン B6 欠乏による衛星細胞および骨 格筋再生への影響2022

    • Author(s)
      ○Kumrungsee Thanutchaporn、田口 花、 矢中 規之
    • Organizer
      第76回日本栄養・食糧学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Effects of GABA transaminase inhibitors on appetite regulation2022

    • Author(s)
      ○Thanutchaporn Kumrungsee,Tomoka Nagao,Noriyuki Yanaka
    • Organizer
      日本農芸化学会2022年度中四国支部大会(第63回講演会)
    • Related Report
      2022 Research-status Report
  • [Presentation] Pursuing Higher Education and Career Development in Japan2022

    • Author(s)
      Thanutchaporn KUMRUNGSEE
    • Organizer
      日本畜産学会第130回大会 若手奨励・男女共同参画推進委員会主催ランチョンセミナー
    • Related Report
      2022 Research-status Report
  • [Presentation] ホモカルノシンの脳機能における生理的役割の探索、およびプレ/プロバイオティクスへの応用2022

    • Author(s)
      Thanutchaporn KUMRUNGSEE
    • Organizer
      第 22 回 DIJF カンファランス 令和 3(2021)年度 ダノン学術研究助成金受贈研究報告会
    • Related Report
      2022 Research-status Report
  • [Presentation] Involvement of blood gamma-aminobutyric acid in appetite regulation2022

    • Author(s)
      ○Thanutchaporn Kumrungsee,長尾 知香,矢中 規之
    • Organizer
      第 55 回 日本栄養・食糧学会 中国・四国支部大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Appetite-suppressing effects of dietary GABA and GABA degradation inhibition in lean and obese mice: a potential strategy for obesity treatment2022

    • Author(s)
      *Thanutchaporn Kumrungsee1, Tomoka Nagao, Noriyuki Yanaka
    • Organizer
      22nd IUNS-International Congress of Nutrition
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Homocarnosine as a novel food factor increases brain GABA and homocarnosine levels2022

    • Author(s)
      *Jason Braga, Thanutchaporn Kumrungsee, Mikako Sato, Yukihiro Sugawara, Noriyuki Yanaka
    • Organizer
      22nd IUNS-International Congress of Nutrition
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Nutrients for sarcopenia prevention and an in vivo imaging model using animals2021

    • Author(s)
      Thanutchaporn Kumrungsee
    • Organizer
      2nd IUFoST-Japan Webinar on Food Functionality
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] BIOSYNTHESIS OF A CARNOSINE-LIKE PEPTIDE IN MOUSE SKELETAL MUSCLES BY DIETARY GAMMA-AMINOBUTYRIC ACID2021

    • Author(s)
      Takumi Komaru, Noriyuki Yanaka, and Thanutchaporn Kumrungsee
    • Organizer
      The 58th Japanese Peptide Symposium
    • Related Report
      2021 Research-status Report
  • [Presentation] 2021年度農芸化学奨励賞受賞者講演:「ヒスチジン含有機能性ペプチドの探索、および応用研究」2021

    • Author(s)
      Thanutchaporn Kumrungsee
    • Organizer
      日本農芸化学会2021
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 2021年度日本農芸化学会 農芸化学奨励賞 受賞講演:「ヒスチジン含有機能性ペプチドの探索、および応用研究」2021

    • Author(s)
      Thanutchaporn Kumrungsee
    • Organizer
      日本農芸化学会中四国支部 第59回講演会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 骨格筋再生と筋サテライト細胞の生理機能における栄養の役割2021

    • Author(s)
      Thanutchaporn Kumrungsee
    • Organizer
      第 4 回 HiHA Young Researchers Workshop
    • Related Report
      2021 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] 脳内のGABA増加剤2021

    • Inventor(s)
      佐藤三佳子 菅原幸博 矢中規之 カムランシータナッチャポーン
    • Industrial Property Rights Holder
      佐藤三佳子 菅原幸博 矢中規之 カムランシータナッチャポーン
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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