| Project/Area Number |
21K14804
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 38050:Food sciences-related
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2021-04-01 – 2024-03-31
|
| Project Status |
Completed (Fiscal Year 2023)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Keywords | carnosine / muscle regeneration / skeletal muscle / vitamin B6 / satellite cells / sarcopenia / muscle atrophy / Carnosine / Muscle regeneration / Skeletal muscle / Vitamin B6 / dexamethasone / Satellite cells / Twist2 |
|---|
| Outline of Research at the Start |
For decades, carnosine has been believed to improve exercise performance and prevent sarcopenia, but its exact molecular mechanisms remain unknown. This study aims to clarify if carnosine has any effects on muscle satellite cells (SCs, Pax7+ cells) and recently identified muscle progenitor cells (PCs, Tw2+ cells), essential for skeletal muscle regeneration and maintaining muscle mass, respectively. Moreover, this study will determine how carnosine plays a role in muscle regeneration and muscle energy metabolism.
|
| Outline of Final Research Achievements |
This study demonstrated that carnosine played a role in decreasing susceptibility of satellite cells (SCs) upon activation and in regulating SC proliferation. Carnosine was found to have less effects on the early stage of muscle regeneration, but play a role in maintaining muscle mass and fiber size during muscle growth or hypertrophy. Vitamin B6 (B6) was confirmed to be a carnosine regulator. B6 deficiency was found to markedly decrease carnosine and its substrate β-alanine levels in both mouse skeletal muscles and C2C12 muscle cells. B6 possibly played a role in maintaining quiescent SC pool and supporting proliferation and self-renewal of SCs. Both B6 deficiency and supplementation delayed muscle regeneration and suppressed muscle hypertrophy. However, B6 supplementation suppressed muscle atrophy by down-regulating MURF-1 and Atrogin-1 expressions, protein-degrading genes. The present study provides new insight into novel roles of carnosine and vitamin B6 in preventing sarcopenia.
|
| Academic Significance and Societal Importance of the Research Achievements |
This research is expected to make a significant contribution to society in terms of extending healthy life expectancy by providing the development of nutritional intervention for preventing sarcopenia, which is an extremely important issue for the aging society.
|
