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Exploring the functional role of Hijiki in obesity-associated to sphingolipid metabolism

Research Project

Project/Area Number 21K14812
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 38050:Food sciences-related
Research InstitutionHokkaido University

Principal Investigator

BommeGowda SiddabasaveGowda  北海道大学, 保健科学研究院, 准教授 (50800504)

Project Period (FY) 2021-04-01 – 2025-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2024: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsHijiki / obesity / Sphingolipid metabolism / lipids / LC/MS / Obesity
Outline of Research at the Start

(1) Isolate and characterize the chemical constituent of Hijiki responsible for SMS inhibition. (2) Determination of its IC50 and mode of action on the lipid droplet formation by cell-based assay (3) Revealing the in vitro effects by analysis of altered cellular lipid biomarkers by LC-MS (4) Elucidate the in vivo effects by oral administration of the active constituent of
Hijiki using High-fat diet mouse model.

Outline of Annual Research Achievements

We achieved significant progress in our research in the last year. We successfully established the sphingomylein synthase (SMS) monitoring assay method using LC/MS and validated by in-vitro and in-silico studies using a known inhibitor for SMS. Also, the manuscript was prepared and submitted to the international peer-reviewed journal. We successfully identified the compound in Hijiki responsible for SMSs nhibition by mass spectrometry and NMR techniques. The identified compound was confirmed with authentic standard. Further, we investigated the inhibition affinity of active compounds with SMS enzymes by docking studies. The summary of results including isolation characterization and SMS assay results are under preparation for a possible manuscript while further studies are in progress.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

Due to the characterization of the active component in Hijiki, there is slight delay in the progress, however it is going rather smoothly now as we successfully characterized the active component of hijiki responsible for SMS inhibition.

Strategy for Future Research Activity

We have now isolated the active component of Hijiki with complete characterization by spectroscopic and spectrometry techniques. Our future goal is to test this active compound in in-vitro and in-vivo system. We will first perform the in-vitro activity of the Hijiki derived SMS inhibitor using fatty liver disease cell model by LC/MS and fluorescence microscopy techniques. The inhibition at cellular levels will be confirmed by measuring sphingomylein lipid in the C3A cells. Based on the in-vitro results, the in-vivo studies will be conducted as planned in the proposal and an overall report will be prepared by the end of the fiscal year. Additionally, the manuscript will be prepared to submit to an international journal and also results will be presented at a domestic conference.

Report

(3 results)
  • 2023 Research-status Report
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (16 results)

All 2024 2023 2022 2021

All Journal Article (11 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Synthesis and quantification of short-chain fatty acid esters of hydroxy fatty acids in rat intestinal contents and fecal samples by LC-MS/MS2024

    • Author(s)
      Gowda Siddabasave Gowda B.、Hou Fengjue、Gowda Divyavani、Chiba Hitoshi、Kawakami Kentaro、Fukiya Satoru、Yokota Atsushi、Hui Shu-Ping
    • Journal Title

      Analytica Chimica Acta

      Volume: 1288 Pages: 342145-342145

    • DOI

      10.1016/j.aca.2023.342145

    • Related Report
      2023 Research-status Report
  • [Journal Article] Dissecting new lipids and their composition in herbal tea using untargeted LC/MS2024

    • Author(s)
      Nath Lipsa Rani、B. Gowda Siddabasave Gowda、Gowda Divyavani、Hou Fengjue、Chiba Hitoshi、Hui Shu Ping
    • Journal Title

      Food Chemistry

      Volume: 447 Pages: 138941-138941

    • DOI

      10.1016/j.foodchem.2024.138941

    • Related Report
      2023 Research-status Report
  • [Journal Article] Sex-Specific Effect of Ethanol on Colon Content Lipidome in a Mice Model Using Nontargeted LC/MS2024

    • Author(s)
      Jayaprakash Jayashankar、B. Gowda Siddabasave Gowda、K. Shukla Pradeep、Gowda Divyavani、Nath Lipsa Rani、Chiba Hitoshi、Rao Radhakrishna、Hui Shu-Ping
    • Journal Title

      ACS Omega

      Volume: 9 Issue: 14 Pages: 16044-16054

    • DOI

      10.1021/acsomega.3c09597

    • Related Report
      2023 Research-status Report
  • [Journal Article] Regio-specific lipid fingerprinting of edible sea cucumbers using LC/MS2024

    • Author(s)
      Minami Yusuke、B. Gowda Siddabasave Gowda、Gowda Divyavani、Chiba Hitoshi、Hui Shu-Ping
    • Journal Title

      Food Research International

      Volume: 184 Pages: 114253-114253

    • DOI

      10.1016/j.foodres.2024.114253

    • Related Report
      2023 Research-status Report
  • [Journal Article] Mass spectrometric approaches in discovering lipid biomarkers for COVID‐19 by lipidomics: Future challenges and perspectives2023

    • Author(s)
      B. Gowda Siddabasave Gowda、Shekhar Chandra、Gowda Divyavani、Chen Yifan、Chiba Hitoshi、Hui Shu‐Ping
    • Journal Title

      Mass Spectrometry Reviews

      Volume: 43 Issue: 5 Pages: 1-25

    • DOI

      10.1002/mas.21848

    • Related Report
      2023 Research-status Report
  • [Journal Article] Lipid Composition Analysis and Characterization of Acyl Sterol Glycosides in Adzuki and Soybean Cultivars by Non-Targeted LC-MS2023

    • Author(s)
      Gangadhara Rachana M.、Gowda Siddabasave Gowda B.、Gowda Divyavani、Inui Ken、Hui Shu-Ping
    • Journal Title

      Foods

      Volume: 12 Issue: 14 Pages: 2784-2784

    • DOI

      10.3390/foods12142784

    • Related Report
      2023 Research-status Report
  • [Journal Article] Crosstalk between Lipids and Non-Alcoholic Fatty Liver Disease2023

    • Author(s)
      Gowda Divyavani、Shekhar Chandra、B. Gowda Siddabasave Gowda、Chen Yifan、Hui Shu-Ping
    • Journal Title

      Livers

      Volume: 3 Issue: 4 Pages: 687-708

    • DOI

      10.3390/livers3040045

    • Related Report
      2023 Research-status Report
  • [Journal Article] Regiospecific analysis of lipidome in the brain from mammals of land and aquatic habitats-by liquid chromatography-mass spectrometry2023

    • Author(s)
      B. Gowda Siddabasave Gowda、Gowda Divyavani、Hou Fengjue、Shekhar Chandra、Chiba Hitoshi、Patzke Nina、Hui Shu-Ping
    • Journal Title

      Heliyon

      Volume: 9 Issue: 12 Pages: e22959-e22959

    • DOI

      10.1016/j.heliyon.2023.e22959

    • Related Report
      2023 Research-status Report
  • [Journal Article] Temporal lipid profiling in the progression from acute to chronic heart failure in mice and ischemic human hearts2022

    • Author(s)
      Gowda SGB, Gowda D, Hou F, Chiba H, Parcha V, Arora P, Halade GV, Hui SP.
    • Journal Title

      Atherosclerosis.

      Volume: 363 Pages: 30-41

    • DOI

      10.1016/j.atherosclerosis.2022.11.005

    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Journal Article] Simple and Sensitive Method for the Quantitative Determination of Lipid Hydroperoxides by Liquid Chromatography/Mass Spectrometry2022

    • Author(s)
      Liang Chongsheng、B. Gowda Siddabasave Gowda、Gowda Divyavani、Sakurai Toshihiro、Sazaki Iku、Chiba Hitoshi、Hui Shu-Ping
    • Journal Title

      Antioxidants

      Volume: 11 Issue: 2 Pages: 229-229

    • DOI

      10.3390/antiox11020229

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Defining the kinetic effects of infection with influenza virus A/PR8/34 (H1N1) on sphingosine-1-phosphate signaling in mice by targeted LC/MS2021

    • Author(s)
      Gowda Divyavani、Ohno Marumi、B. Gowda Siddabasave Gowda、Chiba Hitoshi、Shingai Masashi、Kida Hiroshi、Hui Shu-Ping
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 20161-20161

    • DOI

      10.1038/s41598-021-99765-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 液体クロマトグラフィー質量分析計を用いた食品中の新規脂質の探索2023

    • Author(s)
      Siddabasave Gowda B. Gowda
    • Organizer
      第48回日本医用マススペクトル学会年会
    • Related Report
      2023 Research-status Report
  • [Presentation] Development of an LC/MS assay method for evaluating sphingomyelin synthase inhibition and screening of seaweed extracts: Comprehensive in-vitro and in-silico approaches2023

    • Author(s)
      Jayashankar Jayaprakash (1st), Siddabasave Gowda B Gowda (2nd)
    • Organizer
      第48回日本医用マススペクトル学会年会
    • Related Report
      2023 Research-status Report
  • [Presentation] Analysis of lipidome in brain from animals of land and aquatic habitats-by LC/MS2023

    • Author(s)
      Siddabasave Gowda B. Gowda
    • Organizer
      Japanese Society of Clinical Chemistry
    • Related Report
      2023 Research-status Report
  • [Presentation] Analysis of lipid biomarkers for heart failure by LC/MS2022

    • Author(s)
      Siddabasave Gowda B. Gowda
    • Organizer
      第5回学術集会 / 第2回中性脂肪月間
    • Related Report
      2022 Research-status Report
  • [Presentation] Global lipid fingerprinting for early disease biomarker discovery2021

    • Author(s)
      Siddabasave Gowda B. Gowda
    • Organizer
      The 46th Annual meeting of the Japanese Society for Biomedical Mass Spectrometry
    • Related Report
      2021 Research-status Report
    • Invited

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Published: 2021-04-28   Modified: 2024-12-25  

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