| Project/Area Number |
21K14812
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 38050:Food sciences-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2021-04-01 – 2025-03-31
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| Project Status |
Completed (Fiscal Year 2024)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2024: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | Sphingomyelin synthase / hijki / fatty liver / fatty acid / obesity / Hijiki / Sphingolipid metabolism / lipids / LC/MS / Obesity |
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| Outline of Research at the Start |
(1) Isolate and characterize the chemical constituent of Hijiki responsible for SMS inhibition. (2) Determination of its IC50 and mode of action on the lipid droplet formation by cell-based assay (3) Revealing the in vitro effects by analysis of altered cellular lipid biomarkers by LC-MS (4) Elucidate the in vivo effects by oral administration of the active constituent of
Hijiki using High-fat diet mouse model.
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| Outline of Final Research Achievements |
The major research achievements of this research study include the purification of the active compound present in hijiki response for sphingomylein synthase (SMS) inhibition was achieved. At first, a sensitive method for SMS inhibition assay was developed and published (Analyst, 2024, 149, 3293-3301) by validating with known SMS inhibitors. After preliminary experiments, the methanol extracts of hijiki were prepared in large scale (1kg/2L) and solvent partitioned to find the active component of hijiki responsible for SMS inhibition. Our results showed that hexane fraction 1 as potential inhibitory activity for both SMS1 and SMS2 enzymes. The active compound in hexane fraction 1 was identified and characterized by high-resolution mass spectrometry, NMR, saturated fatty acid which demonstrated an IC50 value of 4.5 and 5.8 micromolar for SMS1 and SMS2 respectively.These in vitro results suggested that hijiki as a potential source for obesity control associated with lipid metabolism.
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| Academic Significance and Societal Importance of the Research Achievements |
Obesity is one of the global epidemic, there is great need for food derived supplements to control the metabolic disease. Our study showed hijiki is a potential seaweed for obesity control. Hence, results accomplished in this research are of great value for the society.
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