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Revealing the regulatory mechanism of GID1 gene expression in a gibberellin high sensitive mutant of grapevine.

Research Project

Project/Area Number 21K14849
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionYamagata University (2022-2023)
Shimane University (2021)

Principal Investigator

Shibuya Tomoki  山形大学, 農学部, 准教授 (60818219)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsジベレリン / 受容体 / ブドウ / 転写制御 / 果実発達 / ジベレリン応答 / 転写因子 / シベレリン応答
Outline of Research at the Start

ブドウの種なし栽培に利用されるジベレリン(GA)に対する植物の応答を考える上で,GAを受容するGID1の機能は極めて重要である.申請者は,ブドウ‘デラウェア’のGA高感受性の突然変異系統において,GID1およびその共役因子であるSLY1の遺伝子発現が通常系統よりも高いことを見出した.本研究では,ブドウ‘デラウェア’のGA高感受性系統における発現変動遺伝子や発現配列の変異を探索し,GAシグナリングの導入部を担う遺伝子群の発現制御機構を検討する.本研究から得られる知見は植物生理学的にも新規で重要なものになるだけでなく,突然変異の態様によっては育種利用に展開し得る.

Outline of Final Research Achievements

Transcriptome analysis was performed to investigate factors contributing to the regulation of GID1 gene expression in gibberellin (GA)-high sensitive mutant of 'Delaware' grape. Among the expression variation genes detected between 'Delaware' and GA-high sensitive mutant in developing berries, I focused on expression variation genes that were commonly detected at different stages of grape development. I found ovate family protein based on its predicted gene function and FEZ based on its extremely variable expression, as a candidate regulator of the GID1 gene. In addition, the 'Delaware' genome was analyzed by long read sequencing to obtain basic information for further research.

Academic Significance and Societal Importance of the Research Achievements

本研究によってOFPやFEZといった転写因子がジベレリン受容体遺伝子の発現制御に関与する可能性が示されたことは植物生理学的にも新しい発見である。特にNAC転写因子であるFEZは、シロイヌナズナでは根での発現と機能を報告した研究があるが、果実発達のモデルであるトマトを含め、他の植物で果実器官における発現および機能が報告されておらず興味深い知見と言える。また、多数の突然変異系統が存在するブドウ‘デラウェア’はブドウにおける形質と遺伝子機能の関連について研究する上で重要な品種であり、そのゲノム情報が得られたことで、育種利用可能な新規知見の開拓に大きく貢献すると考えられる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (10 results)

All 2023 2022

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Effect of GA<sub>3</sub> Treatment on Berry Development in the Large Berry Mutant of ‘Delaware’ Grapes2023

    • Author(s)
      Hikaru Ishikawa, Yasuyuki Togano, Tomoki Shibuya
    • Journal Title

      The Horticulture Journal

      Volume: 92 Issue: 3 Pages: 236-244

    • DOI

      10.2503/hortj.QH-054

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of short-term temperature stress on fruit set and the expression of an auxin reporter gene and auxin synthesis genes in tomato2022

    • Author(s)
      Ito Hikaru、Kanayama Yoshinori、Shibuya Tomoki、Mohammed Seedahmed A.、Nishiyama Manabu、Kato Kazuhisa
    • Journal Title

      Scientia Horticulturae

      Volume: 300 Pages: 111039-111039

    • DOI

      10.1016/j.scienta.2022.111039

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of short-term temperature stress on fruit set and the expression of an auxin reporter gene and auxin synthesis genes in tomato2022

    • Author(s)
      Hikaru Ito,Yoshinori Kanayama,Tomoki Shibuya, Seedahmed A. Mohammed, Manabu Nishiyama, Kazuhisa Kato
    • Journal Title

      Scientia Horticulturae

      Volume: 300 Pages: 111039-111039

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Profile of gene expression at berry enlargement phase in the large berry mutant of ‘Delaware’2023

    • Author(s)
      Hikaru Ishikawa, Yasuyuki Togano, Tomoki Shibuya
    • Organizer
      The 4th Asian Horticultural Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ブドウ‘デラウェア’の大粒系統における果粒発達とGA応答性に関する研究2023

    • Author(s)
      石川ひかる, 栂野康行, 渋谷知暉
    • Organizer
      第40回植物バイオテクノロジー学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ブドウ‘デラウェアの大粒系統におけるジベレリン応答と果粒発達の特性2023

    • Author(s)
      石川ひかる, 栂野康行, 渋谷知暉
    • Organizer
      園芸学会東北支部令和5年度青森大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ブドウ‘デラウェア’の大粒系統におけるGAシグナリングと果実発達の関係2022

    • Author(s)
      石川ひかる・栂野康行・渋谷知暉
    • Organizer
      園芸学会令和 4 年度春季大会
    • Related Report
      2022 Research-status Report 2021 Research-status Report
  • [Presentation] GA signaling and pericarp tissue structure of the large berry mutant of ‘Delaware’2022

    • Author(s)
      Hikaru Ishikawa, Yasuyuki Togano, Tomoki Shibuya
    • Organizer
      The 31st International Horticultural Congress
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] GA受容体遺伝子VvGID1の高発現がブドウの果実肥大におよぼす影響2022

    • Author(s)
      石川ひかる・栂野康行・渋谷知暉
    • Organizer
      園芸学会令和 4 年度秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ブドウ‘デラウェア’の大粒系統の果粒肥大期における遺伝子発現プロファイル2022

    • Author(s)
      石川ひかる・栂野康行・渋谷知暉
    • Organizer
      園芸学会令和 5 年度春季大会
    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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