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Genomic comparisons of cryptic species to investigate interspecific hybridization hotspots in oval squid

Research Project

Project/Area Number 21K14897
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionRegional Fish Institute, Ltd (R&D Division) (2022-2023)
The University of Tokyo (2021)

Principal Investigator

TOMANO Satoshi  リージョナルフィッシュ株式会社(研究開発部), 研究開発部, 研究員 (30866570)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords頭足類 / 種間交雑 / アオリイカ / 遺伝的多様性 / 生殖隔離 / 人工授精 / 異種間交配 / 配偶子 / ゲノム / 系統地理
Outline of Research at the Start

アオリイカは「イカの王様」の異名をもち,商品価値が極めて高い優良資源である。申請者のこれまでの研究によって,アオリイカ類3種は海域によって各種の割合がことなり,さらに3種が同所的に生息する海域が明らかとなった。しかし,3種間の異種間交配の実態や,それが資源や生態系へ与える質的な影響は未知であるため,まずは実態を把握することが急務といえる。本課題では,アオリイカ類における異種間交配の実態を明らかにするため,雑種個体が占める割合を地理的に比較し,ゲノム情報から遺伝子浸透の歴史を推定する。異種間交配の実態を踏まえた上で,各地で行われている人工的な産卵サポートや資源管理を効率的な方策を考察する。

Outline of Final Research Achievements

The purpose of this study was to examine the status of reproductive isolation and introgression among the three species of oval squid through interspecific hybridization tests using artificial insemination and breeding experiments. First of all, it took time to start up the experiment until the second year of the project due to the spread of the coronavirus epidemic and the change of the representative's affiliation. However, we established an artificial insemination method for the oval squid and were able to achieve a fertilization success rate of about 60%. This result is high compared to previous studies and represents a significant advance in artificial insemination technology for squids. Furthermore, artificial insemination among multiple species of the oval squid genus was investigated, and it was suggested that hybridyzation may occur.

Academic Significance and Societal Importance of the Research Achievements

アオリイカにおける人工授精方法を確立し、6割程度の受精成功度を得ることができた。この結果は既往研究に比較して非常に高い値であり,アオリイカ類の人工授精技術を大きく発展させたといえる。さらにアオリイカ属複数種間での人工授精を試み、授精がおきることが示唆された。これは近年注目されているアオリイカ類の完全養殖や、養殖に適した系統の作出に非常に有益である。さらに、本研究で得られた知見の一部は、4件の国際誌、国際学会および国内学会に発表する成果となった。これはイカ類の繁殖生態や集団遺伝学的研究に大きく寄与するものといえる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (11 results)

All 2024 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (2 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Int'l Joint Research] Salk Biological Institute/University of California Los Angeles(米国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] 台湾海洋大学(その他の国・地域)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] Salk Biological Institute/University of California Los Angeles(米国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Males conditionally inseminate at three female body locations according to female mating history and female maturity status in a squid.2024

    • Author(s)
      Kamrun Naher Azad, Md. Nur E. Alam, Makoto Nagata, Satoshi Tomano, Hiroki Ono, Kyoko Sugai, Noritaka Hirohashi
    • Journal Title

      Scientific Reports

      Volume: 14 Issue: 1 Pages: 11702-11702

    • DOI

      10.1038/s41598-024-62062-7

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] he role of the isolation of the marginal seas during the Pleistocene in the genetic structure of black sea bream Acanthopagrus schlegelii (Bleeker, 1854) in the coastal waters of Japan2021

    • Author(s)
      Yutaro Yamashita, Gustavo Sanchez, Kentaro Kawai, Satoshi Tomano, Hiroki Fujita, Tetsuya Umino.
    • Journal Title

      Peer J

      Volume: 9 Pages: e11001-e11001

    • DOI

      10.7717/peerj.11001

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Context-dependent behavioural plasticity compromises disruptive selection of sperm traits in squid2021

    • Author(s)
      Noritaka Hirohashi, Noriyosi Sato, Yoko Iwata, Satoshi Tomano, Md Nur E Alam, Lgia Haselmann Apostlico, Jos; Eduardo Amoroso Rodriguez Marian
    • Journal Title

      Plos One

      Volume: 16 Issue: 8 Pages: 0256745-0256745

    • DOI

      10.1371/journal.pone.0256745

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] All the spermatangia on a female were implanted by single-pair copulation in giant squid Architeuthis dux2021

    • Author(s)
      Riho Murai, Mamiya Shiomi, Masa-aki Yoshida, Satoshi Tomano, Yoko Iwata, Kyoko Sugai, Noritaka Hirohashi
    • Journal Title

      Deep Sea Research Part I: Oceanographic Research Papers

      Volume: 175 Pages: 103585-103585

    • DOI

      10.1016/j.dsr.2021.103585

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Mitochondrial DNA analysis reveals phenotyic plasticity in swordtip squid (Uroteuthis edulis): morphologically different but genetically homogeneous populations2022

    • Author(s)
      Saotshi Tomano
    • Organizer
      Cephalopod International Advisary Council 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヤリイカにおける人工孵化手法の確立2021

    • Author(s)
      笘野哲史・中山新・細野将汰・岩田容子
    • Organizer
      日本水産学会
    • Related Report
      2021 Research-status Report
  • [Remarks] 笘野のホームページ

    • Related Report
      2022 Research-status Report
  • [Remarks] 笘野哲史のページ

    • URL

      https://www.tomano.org

    • Related Report
      2021 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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