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Molecular basis of clonal reproduction in Carassius fish and its application for engineering clonal animals.

Research Project

Project/Area Number 21K14919
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40040:Aquatic life science-related
Research InstitutionKyushu University (2023)
Institute of Physical and Chemical Research (2021-2022)

Principal Investigator

Mishina Tappei  九州大学, 農学研究院, 助教 (40830162)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsフナ / クローン繁殖 / 遺伝基盤 / 減数分裂 / 受精卵 / 染色体分配 / 遺伝子操作 / 前核形成 / 卵母細胞
Outline of Research at the Start

クローン繁殖をする生物の作出は、同一の遺伝背景を持つ生物の迅速な確立や、雑種強勢により生じた有用形質の固定を可能にし、生命科学や農林水産分野での応用が期待される。本研究では、クローン繁殖の責任候補遺伝子が絞り込まれているコイ科フナ属魚類をモデルとして、減数分裂における染色体分配の制御機構および、受精後の核構造の制御機構の理解、その応用によるクローン繁殖動物の作出を目指す。そのために、まず、同定した責任遺伝子および変異が減数分裂過程および受精後の核構造制御で果たす機能を解明する。さらに、同定した変異を人為的にゼブラフィッシュに導入することで、クローン繁殖する系統を作出する。

Outline of Final Research Achievements

Using crucian carp fishes, which are known to reproduce clonally in nature, as a model, we analyzed the "stability of clonal reproduction" and the "molecular basis of clonal reproduction" using an approach that integrates detailed cytological observations, population genome analysis, gene expression analysis, and genetic manipulation experiments. Cytological observations showed that clonal reproduction is a highly stable and consistent trait. Then, the molecular basis for such traits was explored using population genome analysis and gene expression analysis, and candidate genes were obtained. We also analyzed these candidate genes by gene editing (knockout) and artificial gene expression experiments in oocytes, and were able to reproduce the part of the phenotype of clonal reproduction.

Academic Significance and Societal Importance of the Research Achievements

クローン繁殖をする生物の作出は、生命科学や農林水産分野に革新をもたらすと期待される。このクローン繁殖化を動物で実現するには「減数分裂と受精の機構を解明し、適切に遺伝子改変すること」が重要であるが、こうした試みは動物では皆無であった。本研究では、フナ類のゲノム解析等から特定したクローン繁殖の原因候補遺伝子によって、部分的にクローン繁殖の形質を他の魚で再現できた。こうした遺伝子を適切に制御することにより、クローン繁殖動物の作出に加え、ヒトの染色体異常による疾患や不妊に対する基礎医学へと応用できる可能性が見出された。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (12 results)

All 2024 2023 2022 2021 Other

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (3 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Massive horizontal gene transfer and evolution of nematomorph-driven behavioral manipulation of mantids2023

    • Author(s)
      Mishina T, Chiu M-C, Hashiguchi Y, Oishi S, Sasaki A, Okada R, Uchiyama H, Sasaki T, Sakura M, Takeshima H, Sato T
    • Journal Title

      Curr Biol

      Volume: 33 Issue: 22 Pages: 4988-4994.e5

    • DOI

      10.1016/j.cub.2023.09.052

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The detailed population genetic structure of the rare endangered latid fish akame Lates japonicus with extremely low genetic diversity revealed from single-nucleotide polymorphisms2023

    • Author(s)
      Naito Takuya、Nakayama Kouji、Takeshima Hirohiko、Hashiguchi Yasuyuki、Akita Tetsuya、Yamasaki Yo Y.、Mishina Tappei、Takeshita Naohiko、Nagano Atsushi J.、Takahashi Hiroshi
    • Journal Title

      Conservation Genetics

      Volume: online Issue: 4 Pages: 1-13

    • DOI

      10.1007/s10592-023-01517-2

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Origin of scarlet gynogenetic triploid Carassius fish: Implications for conservation of the sexual-gynogenetic complex2022

    • Author(s)
      Mishina Tappei、Nomoto Kazuhiro、Machida Yoshiyasu、Hariu Tsutomu、Watanabe Katsutoshi
    • Journal Title

      PLOS ONE

      Volume: 17 Issue: 10 Pages: e0276390-e0276390

    • DOI

      10.1371/journal.pone.0276390

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 2022年度魚類学会年会シンポジウム「ゲノムが拓く魚類表現型多様性研究の新展開:分野横断的自然史研究と今後の展望」を開催して2022

    • Author(s)
      三品達平・山﨑曜
    • Journal Title

      魚類学雑誌

      Volume: 69 Pages: 252-257

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gudgeon fish with and without genetically determined countershading coexist in heterogeneous littoral environments of an ancient lake2021

    • Author(s)
      Kokita Tomoyuki、Ueno Kohtaro、Yamasaki Yo Y.、Matsuda Masanari、Tabata Ryoichi、Nagano Atsushi J.、Mishina Tappei、Watanabe Katsutoshi
    • Journal Title

      Ecology and Evolution

      Volume: 11 Issue: 19 Pages: 13283-13294

    • DOI

      10.1002/ece3.8050

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single‐oocyte transcriptome analysis reveals aging‐associated effects influenced by life stage and calorie restriction2021

    • Author(s)
      Mishina Tappei、Tabata Namine、Hayashi Tetsutaro、Yoshimura Mika、Umeda Mana、Mori Masashi、Ikawa Yayoi、Hamada Hiroshi、Nikaido Itoshi、Kitajima Tomoya S.
    • Journal Title

      Aging Cell

      Volume: 20 Issue: 8

    • DOI

      10.1111/acel.13428

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization2021

    • Author(s)
      Mori Masashi、Yao Tatsuma、Mishina Tappei、Endoh Hiromi、Tanaka Masahito、Yonezawa Nao、Shimamoto Yuta、Yonemura Shigenobu、Yamagata Kazuo、Kitajima Tomoya S.、Ikawa Masahito
    • Journal Title

      Journal of Cell Biology

      Volume: 220 Issue: 10

    • DOI

      10.1083/jcb.202012001

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interploidy gene flow involving the sexual-asexual cycle facilitates the diversification of gynogenetic triploid Carassius fish2021

    • Author(s)
      Mishina Tappei、Takeshima Hirohiko、Takada Mikumi、Iguchi Kei’ichiro、Zhang Chunguang、Zhao Yahui、Kawahara-Miki Ryouka、Hashiguchi Yasuyuki、Tabata Ryoichi、Sasaki Takeshi、Nishida Mutsumi、Watanabe Katsutoshi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 22485-22485

    • DOI

      10.1038/s41598-021-01754-w

    • NAID

      120007169935

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] フナ類にみられる倍数性変化を伴うクローン繁殖の分子基盤とその安定性2024

    • Author(s)
      三品達平
    • Organizer
      日本生態学会
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] フナ属魚類における雌性発生の遺伝基盤から見えた性の進化的制約2022

    • Author(s)
      三品達平
    • Organizer
      日本魚類学会
    • Related Report
      2022 Research-status Report
  • [Presentation] フナ属魚類におけるクローン繁殖の遺伝基盤から見えた性の進化的制約2022

    • Author(s)
      三品達平
    • Organizer
      有性生殖研究会
    • Related Report
      2022 Research-status Report
  • [Remarks] 天然記念物ヒブナの起源を解明―クローン繁殖のはずなのにキンギョと交雑―

    • URL

      https://www.kyoto-u.ac.jp/ja/research-news/2022-10-21

    • Related Report
      2022 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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