| Project/Area Number |
21K14969
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 42020:Veterinary medical science-related
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| Research Institution | Hokkaido University |
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Principal Investigator |
THAPA JEEWAN 北海道大学, 人獣共通感染症国際共同研究所, 助教 (40837449)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | Tuberculosis / Mycobacterium orygis / Pathogenomics / Molecular epidemiology / Nepal / tuberculosis / molecular epidemiology / epidemiology |
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| Outline of Research at the Start |
Mycobacterium orygis is an emerging causative agent of zoonotic tuberculosis (TB) and animal TB, and is epidemiologically associated with the South Asian region. However, how and why does M. orygis causes TB differently from other members of M. tuberculosis complex bacteria is still unknown. We will understand TB disease by taking M. orygis as a model. In addition, molecular epidemiological surveillance across the wildlife/livestock/human interface in Nepal will be conducted to understand the eco-epidemiology of M. orygis.
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| Outline of Final Research Achievements |
Our comparative whole genome analysis of Mycobacterium orygis revealed that it is endemically distributed in South Asia, and there is a threat from Mycobacterium orygis-associated tuberculosis in South Asia and beyond. We did genome analysis of Mycobacterium orygis that was isolated from rhinoceros in Nepal, and the genome analysis showed that it had Mycobacterium orygis specific mutation in anti-SigK factor, which is responsible for higher production of mpt70 and mpt83. We found that Mycobacterium orygis isolates have specific polymorphisms in gyrB, mmpL6, PPE55, Rv2042c, TbD1. We developed a PCR diagnostic method targeting RD301 region that can specifically detect Mycobacterium orygis by differentiating it from other members of Mycobacterium tuberculosis complex. We performed molecular epidemiological surveillance to understand tuberculosis eco-epidemiology across the human/livestock/wildlife interface in Nepal.
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| Academic Significance and Societal Importance of the Research Achievements |
The research identified that the Mycobacterium orygis is an important causative agent of zoonotic tuberculosis (TB) in south Asia region, and there is a threat from Mycobacterium orygis-associated TB in south Asia and beyond.
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