Study for pathogenesis of bat-borne orthoreovirus by genetic comparison between wild-type strains

• Project/Area Number: 21K14970
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Tokyo University of Agriculture and Technology (2022-2023); Hokkaido University (2021)
• Principal Investigator: Harima Hayato 東京農工大学, (連合)農学研究科(研究院), 助教 (70805407)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2023: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ネルソンベイオルソレオウイルス / 病原性 / 遺伝子改変技術 / 野生株 / 宿主との相互作用

Outline of Research at the Start

コウモリが保有するネルソンベイオルソレオウイルス（NBV）はヒトに重篤な呼吸器疾患を引き起こす原因ウイルスであり、新興感染症として注目されている。NBVのウイルス学的性状は未だ不明な点が多く、NBVの治療法やワクチンは確立されていない。本研究では、NBVの野生株間の病原性の違いに着眼し、遺伝子改変技術を駆使することでNBVの病原性を規定するウイルス因子を同定する。さらに、ウイルス因子と相互作用する宿主因子を探索し、その機能解析を進めることでNBVの病原性発現機構を解明する。

Outline of Final Research Achievements

The control of Nelson Bay orthoreovirus (NBV) infection, which causes severe respiratory diseases in humans, has not progressed well, and it is necessary to uncover the mechanism of the pathogenesis of NBV for the development of the cure and vaccine. In this study, we performed the analyses focused on the differences in virulence between high- and low-pathogenic NBV strains and identified two novel virulence factors of NBV. We also demonstrated that the specific domain on one of the virulence factors was required for the pathogenesis of NBV.

Academic Significance and Societal Importance of the Research Achievements

公衆衛生上のネルソンベイオルソレオウイルス（NBV）感染症の発生や流行リスクを評価するために、また、NBVがヒトへの病原性をどのように獲得したのかを明らかにするためには、NBVの病原性発現機構を解明していくことが必要である。本研究では、病原性の異なる2つのNBV野生株及び先進のNBV遺伝子改変技術を用いた組換えNBV解析技術を利用した新たな取り組みによって、NBVの病原性発現機構を解明した。本研究成果はNBV感染症対策のための治療薬やワクチンの開発に向けた基礎的知見となり、NBVの感染制御基盤確立への貢献が期待される。

Research Products

• [Int'l Joint Research] ザンビア大学獣医学部(ザンビア)
• [Int'l Joint Research] ザンビア大学獣医学部(ザンビア)
• [Int'l Joint Research] ザンビア大学獣医学部(ザンビア)
• [Journal Article] Role of multidrug resistance and co-resistance on a high percentage of streptomycin resistance in <i>Escherichia coli</i> isolated from chicken meats in Japan (2023)
  • Author(s): LIN Thein、NOMURA Shizuka、SOMENO Suzuka、ABE Takahiro、NISHIYAMA Miyuki、SHIKI Shunya、HARIMA Hayato、ISHIHARA Kanako
  • Journal Title: Journal of Veterinary Medical Science Volume: 85 Issue: 8 Pages: 832-836
  • DOI: 10.1292/jvms.23-0135
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access
• [Journal Article] Surveillance, Isolation, and Genetic Characterization of Bat Herpesviruses in Zambia (2023)
  • Author(s): Harima H, Qiu Y, Yamagishi J, Kajihara M, Changula K, Okuya K, Isono M, Yamaguchi T, Ogawa H, Nao N, Sasaki M, Simulundu E, Mweene AS, Sawa H, Ishihara K, Hang’ombe BM, Takada A
  • Journal Title: Viruses Volume: 15 Issue: 6 Pages: 1369-1369
  • DOI: 10.3390/v15061369
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Expanding diversity of bunyaviruses identified in mosquitoes (2023)
  • Author(s): Orba Y, Abu YE, Chambaro HM, Lundu T, Muleya W, Eshita Y, Qiu Y, Harima H, Kajihara M, Mori-Kajihara A, Matsuno K, Sasaki M, Hall WW, Hang’ombe BM, Sawa H
  • Journal Title: Scientific Reports Volume: 13 Issue: 1 Pages: 18165-18165
  • DOI: 10.1038/s41598-023-45443-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prevalence and Genomic Characterization of Rotavirus A from Domestic Pigs in Zambia: Evidence for Possible Porcine?Human Interspecies Transmission (2023)
  • Author(s): Ndebe J, Harima H, Chambaro HM, Sasaki M, Yamagishi J, Kalonda A, Shawa M, Qiu Y, Kajihara M, Takada A, Sawa H, Saasa N, Simulundu E
  • Journal Title: Pathogens Volume: 12 Issue: 10 Pages: 1199-1199
  • DOI: 10.3390/pathogens12101199
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation and Characterization of Distinct Rotavirus A in Bat and Rodent Hosts (2023)
  • Author(s): Kishimoto M, Kajihara M, Tabata K, Itakura Y, Toba S, Ozono S, Sato Y, Suzuki T, Ito N, Changula K, Qiu Y, Mori-Kajihara A, Eto Y, Harima H, Mwizabi D, Hang’ombe BM, Hall WW, Takada A, Orba Y, Sawa H, Sasaki M
  • Journal Title: Journal of Virology Volume: 97 Issue: 1
  • DOI: 10.1128/jvi.01455-22
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of Old and New World Relapsing Fever Borreliae in Ornithodoros Ticks Collected from Warthog Burrows in Zambia (2023)
  • Author(s): Qiu Y, Chambaro HM, Sato K, Squarre D, Simulundu E, Kajihara M, Changula K, Simbotwe M, Harima H, Ndebe J, Moonga L, Nakao R, Takada A, Hang’ombe BM, Sawa H, Kawabata H
  • Journal Title: Microorganisms Volume: 11 Issue: 1 Pages: 200-200
  • DOI: 10.3390/microorganisms11010200
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Current status and molecular epidemiology of rabies virus from different hosts and regions in Malawi (2023)
  • Author(s): Kainga H, Chatanga E, Phonera MC, Kothowa JP, Dzimbiri P, Kamwendo G, Mulavu M, Khumalo CS, Changula K, Chambaro H, Harima H, Kajihara M, Mkandawire K, Chikungwa P, Chulu J, Njunga G, Chitanga S, Mubemba B, Sasaki M, Orba Y, Qiu Y, Yamagishi J, Simulundu E, Takada A, Namangala B, Sawa H, Muleya W
  • Journal Title: Archives of Virology Volume: 168 Issue: 2 Pages: 61-61
  • DOI: 10.1007/s00705-022-05635-z
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isolation and characterization of an orthoreovirus from Indonesian fruit bats (2022)
  • Author(s): Intaruck Kittiya、Itakura Yukari、Kishimoto Mai、Chambaro Herman M.、Setiyono Agus、Handharyani Ekowati、Uemura Kentaro、Harima Hayato、Taniguchi Satoshi、Saijo Masayuki、Kimura Takashi、Orba Yasuko、Sawa Hirofumi、Sasaki Michihito
  • Journal Title: Virology Volume: 575 Pages: 10-19
  • DOI: 10.1016/j.virol.2022.08.003
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] An unusually long Rift valley fever inter-epizootic period in Zambia: Evidence for enzootic virus circulation and risk for disease outbreak (2022)
  • Author(s): Chambaro Herman M.、Hirose Kazuyo、Sasaki Michihito、Libanda Brigadier、Sinkala Yona、Fandamu Paul、Muleya Walter、Banda Fredrick、Chizimu Joseph、Squarre David、Shawa Misheck、Qiu Yongjin、Harima Hayato、Eshita Yuki、Simulundu Edgar、Sawa Hirofumi、Orba Yasuko
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 16 Issue: 6 Pages: e0010420-e0010420
  • DOI: 10.1371/journal.pntd.0010420
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Screening of tick-borne pathogens in argasid ticks in Zambia: Expansion of the geographic distribution of Rickettsia lusitaniae and Rickettsia hoogstraalii and detection of putative novel Anaplasma species. (2021)
  • Author(s): Qiu Y, Simuunza M, Kajihara M, Chambaro H, Harima H, Eto Y, Simulundu E, Squarre D, Torii S, Takada A, Hangmbe BM, Sawa H, Sugimoto C, Nakao R
  • Journal Title: Ticks Tick Borne Dis Volume: 12 Issue: 4 Pages: 101720-101720
  • DOI: 10.1016/j.ttbdis.2021.101720
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Attenuated infection by a Pteropine orthoreovirus isolated from an Egyptian fruit bat in Zambia (2021)
  • Author(s): Harima Hayato、Sasaki Michihito、Orba Yasuko、Okuya Kosuke、Qiu Yongjin、Wastika Christida E.、Changula Katendi、Kajihara Masahiro、Simulundu Edgar、Yamaguchi Tomoyuki、Eto Yoshiki、Mori-Kajihara Akina、Sato Akihiko、Taniguchi Satoshi、Takada Ayato、Saijo Masayuki、Hang’ombe Bernard M.、Sawa Hirofumi
  • Journal Title: PLOS Neglected Tropical Diseases Volume: 15 Issue: 9 Pages: e0009768-e0009768
  • DOI: 10.1371/journal.pntd.0009768
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Serological and molecular epidemiological study on swine influenza in Zambia (2021)
  • Author(s): Harima Hayato、Okuya Kosuke、Kajihara Masahiro、Ogawa Hirohito、Simulundu Edgar、Bwalya Eugene、Qiu Yongjin、Mori‐Kajihara Akina、Munyeme Musso、Sakoda Yoshihiro、Saito Takehiko、Hang'ombe Bernard M.、Sawa Hirofumi、Mweene Aaron S.、Takada Ayato
  • Journal Title: Transboundary and Emerging Diseases Volume: - Issue: 4
  • DOI: 10.1111/tbed.14373
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Hepatitis E virus infection in pigs: a first report from Zambia (2021)
  • Author(s): Chambaro Herman M.、Sasaki Michihito、Muleya Walter、Kajihara Masahiro、Shawa Misheck、Mwape Kabemba E.、Harima Hayato、Qiu Yongjin、Hall William W.、Fandamu Paul、Squarre David、Simulundu Edgar、Sawa Hirofumi、Orba Yasuko
  • Journal Title: Emerging Microbes &amp; Infections Volume: 10 Issue: 1 Pages: 2169-2172
  • DOI: 10.1080/22221751.2021.2002669
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] ザンビアのコウモリから分離した新規ガンマヘルペスウイルスの全ゲノム解析 (2023)
  • Author(s): 播磨 勇人、邱 永晋、山岸 潤也、梶原 将大、Katendi Changula、佐々木 道仁、Aaron S Mweene、澤 洋文、石原 加奈子、Bernard M. Hang’ombe、高田 礼人
  • Organizer: 第36回ヘルペスウイルス研究会
• [Presentation] ザンビアのコウモリが保有する多様なヘルペスウイルスの遺伝学的性状の解明 (2023)
  • Author(s): 播磨 勇人、邱 永晋、山岸 潤也、梶原 将大、Katendi Changula、佐々木 道仁、Aaron S Mweene、澤 洋文、石原 加奈子、Bernard M. Hang’ombe、高田 礼人
  • Organizer: 第70回日本ウイルス学会学術集会
• [Presentation] ザンビアにおけるブタインフルエンザの疫学調査 (2023)
  • Author(s): 播磨 勇人、奥谷 公亮、梶原 将大、小川 寛人、Edgar Simulundu、Eugene Bwalya、邱 永晋、迫田 義博、西藤 岳彦、Bernard M. Hang’ombe、澤 洋文、Aaron S Mweene、高田 礼人
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Isolation and Characterization of a Nelson Bay Orthoreovirus from an Egyptian Fruit Bat in Zambia (2022)
  • Author(s): Hayato Harima、Masayuki Saijo、Bernard M Hang’ombe、Hirofumi Sawa
  • Organizer: 14th International dsRNA Virus Symposium
  • Int'l Joint Research
• [Presentation] ザンビアのコウモリにおけるネルソンベイオルソレオウイルスの保有調査および分離ウイルスの病原性比較解析 (2022)
  • Author(s): 播磨 勇人、佐々木 道仁、大場 靖子、邱 永晋、Katendi Changula、梶原 将大、Edgar Simulundu、谷口 怜、高田 礼人、西條 政幸、Bernard M. Hang’ombe、澤 洋文
  • Organizer: 第165回日本獣医学会学術集会
• [Presentation] ザンビアのブタにおける消化管ウイルスの疫学調査 (2021)
  • Author(s): 播磨 勇人、梶原 将大、佐々木 道仁、Edgar Simulundu、Eugene Bwalya、邱 永晋、奥谷 公亮、大場 靖子、Bernard M Hang’ombe、Aaron S Mweene、高田 礼人、澤 洋文
  • Organizer: 第164回日本獣医学会学術集会
• [Presentation] ザンビアにおけるコウモリ由来病原性レオウイルスに関する疫学調査とウイルス株間比較による性状解析 (2021)
  • Author(s): 播磨 勇人、佐々木 道仁、Bernard M. Hang’ombe、澤 洋文
  • Organizer: 第7回北海道大学部局横断シンポジウム
• [Presentation] ザンビアのエジプトルーセットオオコウモリから単離したネルソンベイオルソレオウイルスの性状解析 (2021)
  • Author(s): 播磨 勇人、佐々木 道仁、大場 靖子、邱 永晋、Katendi Changula、梶原 将大、Edgar Simulundu、谷口 怜、高田 礼人、西條 政幸、Bernard M. Hang’ombe、澤 洋文
  • Organizer: 第68回日本ウイルス学会学術集会
• [Presentation] ザンビアで採集したオオコウモリから単離したオルソレオウイルスの解析 (2021)
  • Author(s): 播磨 勇人、佐々木 道仁、大場 靖子、邱 永晋、梶原 将大、谷口 怜、高田 礼人、西條 政幸、澤 洋文
  • Organizer: 第44回日本分子生物学会年会