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Development of a method for prediction of bull semen fertility focusing on the structures of sperm motility subpopulation

Research Project

Project/Area Number 21K14987
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionKitasato University

Principal Investigator

Kanno Chihiro  北里大学, 獣医学部, 助教 (20834469)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords精子 / 牛 / 人工授精 / 妊孕性 / 運動性サブポピュレーション
Outline of Research at the Start

本研究の目的は、牛精子の妊孕性を判定することが可能な実験室的評価方法を確立することである。牛の生産にはほとんどが凍結精液が利用されている。凍結精液の品質管理を目的として、様々な精子性状の検査が行われているが、人工授精受胎率を予測できるような手法は無い。高妊孕性精子の特徴が不明な状況では、凍結精液の品質改善において、どのような精液を作製すべきかを決めることができない。そこで本研究では、精子の運動様式をはじめ、細胞機能、寿命、受精能獲得状況などにより精子性状を判定し、生産現場における人工授精受胎率との関係を解析し、人工授精受胎率の良好な精子、すなわち高妊孕性精子の特徴を決定することを目指す。

Outline of Final Research Achievements

In this study, we analyzed the relationship between bovine sperm quality, including sperm motility subpopulation, and semen fertility to clarify the characteristics of highly fertile sperm.As a result, sperm in semen with high conception rate in artificial insemination (AI) showed linearly moving with high beat-cross frequency, maintain high mitochondrial activity even after thawing of frozen semen. This suggests sperm with linear movement have a long life span and high fertility. On the other hand, sperm in semen with a low conception rate in AI moved curvilinearly with large amplitude of head. These sperm could not maintain cell membrane addition integrity after a long time after thawing of semen. The results of this study show some of the factors that make it possible to predict the fertility of semen from cattle without AI tests in the field.

Academic Significance and Societal Importance of the Research Achievements

これまで、精子の実験室的検査は広く行われてきた一方、その妊孕性を高精度に予測可能な手法は無かった。本研究の成果から、牛における高妊孕性精子の特徴の一端を解明することができた。今後、学術的には精子の運動様式の差異を生み出す機構の解明、社会的には人工授精所における精液検査の手法や新規凍結精液作製による、精液の品質管理および向上に、それぞれ繋がっていく知見であると考えられる。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (18 results)

All 2023 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (9 results) (of which Invited: 1 results)

  • [Int'l Joint Research] Hanwoo Research Institute(韓国)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 牛人工授精用凍結精液に関する最新の知見と技術革新2023

    • Author(s)
      菅野智裕
    • Journal Title

      産業動物臨床医学雑誌

      Volume: 14

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arnold Chiari malformation classified as Chiari 1.5 malformation in a Japanese black calf2023

    • Author(s)
      SATO Shogo、KAKIZAKI Takehiko、KONDO Kao、KANNO Chihiro、MAEDA Yosuke、ANDO Ryo、PARK Chun-Ho、KAWAGUCHI Hiroaki、TAKAHASHI Fumiaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 85 Issue: 5 Pages: 546-550

    • DOI

      10.1292/jvms.22-0463

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Accidental laceration of the vaginal wall by an intravaginal thermometer as a calving detection device in a Japanese black cow2023

    • Author(s)
      KANNO Chihiro、SATO Shogo、KUSAKA Hiromi、MAEDA Yosuke、TAKAHASHI Fumiaki
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 85 Issue: 3 Pages: 363-366

    • DOI

      10.1292/jvms.22-0511

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between frame rates and subpopulation structure of bovine sperm divided by their motility analyzed by a computer‐assisted sperm analysis system2022

    • Author(s)
      Kanno Chihiro、Sun‐Sik Kang、Sakamoto Kentaro Q.、Yanagawa Yojiro、Katagiri Seiji、Nagano Masashi
    • Journal Title

      Animal Science Journal

      Volume: 93 Issue: 1

    • DOI

      10.1111/asj.13796

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Relationship between the timing of insemination based on estrus detected by the automatic activity monitoring system and conception rates using sex-sorted semen in Holstein dairy cattle2022

    • Author(s)
      FURUKAWA Eri、KANNO Chihiro、YANAGAWA Yojiro、KATAGIRI Seiji、NAGANO Masashi
    • Journal Title

      Journal of Reproduction and Development

      Volume: 68 Issue: 4 Pages: 295-298

    • DOI

      10.1262/jrd.2022-006

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oesophageal obstruction caused by ingestion of rice chaff used as bedding material in four Japanese black calves2022

    • Author(s)
      Takahashi Fumiaki、Kanno Chihiro、Maeda Yosuke、Sato Shogo、Takai Shinji
    • Journal Title

      Veterinary Record Case Reports

      Volume: 10 Issue: 4

    • DOI

      10.1002/vrc2.477

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (<i>Macaca fuscata</i>)2022

    • Author(s)
      TAKAESU Noboru、KANNO Chihiro、SUGIMOTO Kosuke、NAGANO Masashi、KANEKO Akihisa、INDO Yoriko、IMAI Hiroo、HIRAI Hirohisa、OKAMOTO Munehiro、SASHIKA Mariko、SHIMOZURU Michito、KATAGIRI Seiji、TSUBOTA Toshio、YANAGAWA Yojiro
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 84 Issue: 3 Pages: 429-438

    • DOI

      10.1292/jvms.21-0590

    • NAID

      130008143967

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zearalenone interferes with the sperm-triggered inflammation in the bovine uterus in vitro: Negative impact on sperm motility and survival2022

    • Author(s)
      Elweza Ahmed E.、Marey Mohamed A.、Elesh Ibrahim F.、Zinnah Mohammad A.、Akthar Ihshan、Kanno Chihiro、Takagi Mitsuhiro、Miyamoto Akio
    • Journal Title

      Reproductive Toxicology

      Volume: 107 Pages: 81-89

    • DOI

      10.1016/j.reprotox.2021.12.001

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 牛凍結精液中の精子運動性サブポピュレーション構成が人工授精受胎率に与える影響2022

    • Author(s)
      村田有加, 髙橋芳幸, 早川宏之, 佐藤将伍, 前田洋佑, 髙橋史昭, 菅野智裕
    • Organizer
      第165回日本獣医学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] アルパカの分娩予測法確立に向けた妊娠雌個体における糞中プロジェステロン濃度および体温推移の検討2022

    • Author(s)
      荒古江美紗, 柳川洋二郎, 前田洋佑, 髙橋史昭, 佐藤将伍, 桃沢健二, 永野昌志, 菅野智裕
    • Organizer
      第165回日本獣医学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 犬凍結精液希釈液へのL-アラニルグルタミン添加が融解後の精液性状に及ぼす影響2022

    • Author(s)
      西尾千尋, 菊池元宏, 菅野智裕, 日下裕美, 三浦弘, 坂口実
    • Organizer
      第165回日本獣医学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 持続可能な畜産を目指した循環型畜産システムが日本短角種繁殖雌牛の消化管内細菌叢に与える影響2022

    • Author(s)
      松原里奈, 近藤香央, 小泉和生, 中村円香, 佐々木汐帆, 石戸谷和季, 田村慎之介, 小笠原英毅, 佐藤将伍, 前田洋佑, 菅野智裕, 髙橋史昭
    • Organizer
      第165回日本獣医学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 日本におけるアルパカの分娩と新生子発育に関する回顧的調査2022

    • Author(s)
      荒古江美紗, 菅野智裕, 佐藤将伍, 前田洋佑, 髙橋史昭, 桃沢健二, 永野昌志
    • Organizer
      第71回東北畜産学会宮城大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 授精作業時における受胎率低下の要因とは? ~授精師のストレス・自己評価編~2022

    • Author(s)
      籾倉茂朗, 宍戸里帆, 大泉諭士, 秋山麗人, 河田亜胡, 中川健太郎, 中神丈浩, 三木貴史, 菅野智裕, 椋代公貴
    • Organizer
      第78回北海道家畜人工授精技術研修大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] クラスター解析を用いた牛精子における運動様式評価 -精子運動性サブポピュレーションに着目した受胎性予測法の開発-2022

    • Author(s)
      菅野智裕
    • Organizer
      日本アンドロロジー学会第41回学術大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] ホルスタイン種および黒毛和種由来凍結精液中の精子性状の比較2021

    • Author(s)
      菅野智裕, 伊藤陽輔, 髙橋健一
    • Organizer
      第164回日本獣医学会学術集会
    • Related Report
      2021 Research-status Report
  • [Presentation] ホルスタイン種雌牛への追い移植時における非黄体側移植の実用性の検討2021

    • Author(s)
      行田高弘, 宍戸里帆, 籾倉茂朗, 大泉諭士, 新国大河, 工藤絢, 中神 丈浩, 三木貴史, 菅野智裕, 齋藤哲也
    • Organizer
      第77回北海道家畜人工授精技術研修大会 2021年10月15日
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2024-01-30  

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