| Project/Area Number |
21K15088
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 44010:Cell biology-related
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| Research Institution | Hiroshima University |
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Principal Investigator |
Ijaz Faryal 広島大学, 医系科学研究科(医), 助教 (80845595)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | primary CIlium / Primary Cilium / Ciliopathies / Humoral Factors / primary cilium / Extracellular vesicles / Extracellular Vesicles |
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| Outline of Research at the Start |
Primary cilium have recently been shown to be a source of new type of extracellular vesicle (pcEVs).This study aims to identify the molecules and pathways involved in the uptake of pcEVs which will provide insights into the regulation of pcEVs uptake in physiological and pathological conditions.
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| Outline of Final Research Achievements |
In this study, we examined the influence of extracellular bioactive factors derived from the culture supernatant of wildtype NIH/3T3 cells on the migratory and proliferative capacities of primary cilium-deficient NIH/3T3-Kif3a-KO cells after wounding. The factors derived from the culture supernatant of wildtype NIH/3T3 cells increased the rate of cell migration/proliferation in target cells compared to the factors derived from the culture supernatant of primary cilium-deficient NIH/3T3-Kif3a-KO cells. Additionally, through omics analysis of the supernatants, we identified a specific molecular constituent exhibiting elevated concentrations in the culture supernatant of primary cilium-deficient cells. These findings suggest that fibroblasts regulate the cellular migration/ proliferation process in primary cilium-deficient target cells by humoral bioactive factors in a primary cilium-dependent manner.
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| Academic Significance and Societal Importance of the Research Achievements |
繊毛症における繊毛欠損細胞の細胞内因性決定因子の解明は大きく前進しましたが、繊毛欠損細胞からの細胞外因性因子がこれらの病態に及ぼす影響については、まだ比較的未開拓の領域です。この研究は、繊毛症が初めて細胞外因性の観点から研究されたという意味で重要です。これは、将来、繊毛症を治療するための治療法を見つける道を開くものでもあります。
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