• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Specificities of cellular uptake of primary cilium-derived extracellular vesicles for intercellular communication

Research Project

Project/Area Number 21K15088
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44010:Cell biology-related
Research InstitutionHiroshima University

Principal Investigator

Ijaz Faryal  広島大学, 医系科学研究科(医), 助教 (80845595)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsprimary CIlium / Primary Cilium / Ciliopathies / Humoral Factors / primary cilium / Extracellular vesicles / Extracellular Vesicles
Outline of Research at the Start

Primary cilium have recently been shown to be a source of new type of extracellular vesicle (pcEVs).This study aims to identify the molecules and pathways involved in the uptake of pcEVs which will provide insights into the regulation of pcEVs uptake in physiological and pathological conditions.

Outline of Final Research Achievements

In this study, we examined the influence of extracellular bioactive factors derived from the culture supernatant of wildtype NIH/3T3 cells on the migratory and proliferative capacities of primary cilium-deficient NIH/3T3-Kif3a-KO cells after wounding. The factors derived from the culture supernatant of wildtype NIH/3T3 cells increased the rate of cell migration/proliferation in target cells compared to the factors derived from the culture supernatant of primary cilium-deficient NIH/3T3-Kif3a-KO cells. Additionally, through omics analysis of the supernatants, we identified a specific molecular constituent exhibiting elevated concentrations in the culture supernatant of primary cilium-deficient cells. These findings suggest that fibroblasts regulate the cellular migration/ proliferation process in primary cilium-deficient target cells by humoral bioactive factors in a primary cilium-dependent manner.

Academic Significance and Societal Importance of the Research Achievements

繊毛症における繊毛欠損細胞の細胞内因性決定因子の解明は大きく前進しましたが、繊毛欠損細胞からの細胞外因性因子がこれらの病態に及ぼす影響については、まだ比較的未開拓の領域です。この研究は、繊毛症が初めて細胞外因性の観点から研究されたという意味で重要です。これは、将来、繊毛症を治療するための治療法を見つける道を開くものでもあります。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (5 results)

All 2023 2022

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins2023

    • Author(s)
      Nakazato Ryota、Otani Hiroshi、Ijaz Faryal、Ikegami Koji
    • Journal Title

      Methods in Cell Biology

      Volume: 175 Pages: 45-68

    • DOI

      10.1016/bs.mcb.2022.10.003

    • ISBN
      9780443185861
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution2022

    • Author(s)
      Ijaz Faryal、Nakazato Ryota、Setou Mitsutoshi、Ikegami Koji
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 11681-11681

    • DOI

      10.1038/s41598-022-15776-5

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Primary Cilium-dependent Humoral Bioactive Factors Control Fibroblast Cell Migration and Proliferation2023

    • Author(s)
      Faryal Ijaz
    • Organizer
      The 75th Annual Meeting of the Japan Society for Cell Biology
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Primary Cilium-dependent Control of Fibroblast Cell Migration and Proliferation by Humoral Bioactive Factors2023

    • Author(s)
      Faryal Ijaz
    • Organizer
      13th Cilia Society Meeting
    • Related Report
      2023 Annual Research Report
  • [Presentation] Double-strand break Site-Targeted PCR coupled with high concentration TBE-PAGE enables to detect NHEJ-mediated indel mutations at a 1-bp resolution2022

    • Author(s)
      Faryal Ijaz
    • Organizer
      The 74th Annual Meeting of the Japan Society for Cell Biology
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi