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Protein import into endosymbionts becoming organelles

Research Project

Project/Area Number 21K15094
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44010:Cell biology-related
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

Husnik Filip  沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordssymbiosis / metabolomics / proteomics / FIB-SEM / uCT / SBF-SEM / genomics / FISH / bacteria / insect / organelle / 3D microscopy / insects / immunohistochemistry / 3D imaging / protein import / evolution
Outline of Research at the Start

Long-term endosymbiosis results in massive dependence on the host. Mitochondria and plastids, as the oldest known endosymbionts, are well-recognized to rely on import of hundreds of proteins from the host cytoplasm. However, recent results, including my preliminary data, show that proteins can be (by an unknown mechanism) also imported into endosymbionts with highly reduced genomes, blurring the line between endosymbionts and organelles. A fundamental question I will target here is if the protein import can be as massive as in organelles.

Outline of Final Research Achievements

In the frame of the project, I have focused on host-symbiont integration in a unique bacterium-within-bacterium-within-eukaryote symbiosis found in mealybugs. Via the combination of omics (genomics, transcriptomics, proteomics, and metabolomics) with 3D imaging (uCT, confocal FISH imaging, SBF-SEM, FIB-SEM), we managed to understand which genes, proteins, and metabolites are localized in the system and how they drive the tripartite interaction. Integrating data from all these advanced methods is unprecedented for a single symbiotic species and allows us to draw strong conclusions about host-symbiont integration (e.g. cell division coordination and protein import) in highly interdependent, organelle-like, symbioses.

Academic Significance and Societal Importance of the Research Achievements

Understanding how highly integrated symbioses function is essential(and relates to human health) since one organelle in our cells, the mitochondrion, has originated from a symbiotic bacterium.

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (12 results)

All 2023 2022 2021

All Journal Article (4 results) Presentation (8 results) (of which Int'l Joint Research: 5 results,  Invited: 7 results)

  • [Journal Article] Organellogenesis: Host proteins control symbiont cell divisions2023

    • Author(s)
      Husnik Filip
    • Journal Title

      Current Biology

      Volume: 33 Issue: 1 Pages: R22-R25

    • DOI

      10.1016/j.cub.2022.11.028

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Pseudofinder: detection of pseudogenes in prokaryotic genomes2022

    • Author(s)
      Syberg-Olsen MJ, Graber AI, Keeling PJ, McCutcheon JP, Husnik F
    • Journal Title

      Molecular Biology and Evolution

      Volume: 33 Issue: 7

    • DOI

      10.1093/molbev/msac153

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Bacterial and archaeal symbioses with protists2021

    • Author(s)
      Husnik et al.
    • Journal Title

      Current Biology

      Volume: 31 Issue: 13 Pages: 862-877

    • DOI

      10.1016/j.cub.2021.05.049

    • Related Report
      2021 Research-status Report
  • [Journal Article] Pseudofinder: detection of pseudogenes in prokaryotic genomes2021

    • Author(s)
      Syberg-Olsen et al.
    • Journal Title

      biorXiv

      Volume: in press

    • DOI

      10.1101/2021.10.07.463580

    • Related Report
      2021 Research-status Report
  • [Presentation] Organellogenesis: Provide, Divide, and Rule2023

    • Author(s)
      Filip Husnik
    • Organizer
      ERATO Evolving Symbiosis Project International Seminar Series #21
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Two billion years of protist symbioses.2023

    • Author(s)
      Filip Husnik
    • Organizer
      International Society of Protistology (ISOP) student meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Organellogenesis: Provide, Divide, and Rule.2023

    • Author(s)
      Filip Husnik
    • Organizer
      International Society of Evolutionary Protistology (ISEP) biennial meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Bacterial and archaeal symbioses with protists.2022

    • Author(s)
      Filip Husnik
    • Organizer
      EMBO Workshop on Comparative genomics of unicellular eukaryotes: Interactions and symbioses.
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Symbiont replacements are the rule rather than the exception in scale insects2022

    • Author(s)
      Choi, J., Palanichamy, P., Masukagami, Y., Tejeda, J.A., Husnik, F.
    • Organizer
      10th Congress of the International Society of Symbiosis
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bacterial and archael symbioses with protists2021

    • Author(s)
      Filip Husnik
    • Organizer
      The DOE JGI Symposium ‘From New Lineages of Life to New Functions’
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Bacterial and archael symbioses with protists2021

    • Author(s)
      Filip Husnik
    • Organizer
      The 34th Annual Meeting of the Japanese Society of Microbial Ecology
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Bacterial and archael symbioses with protists2021

    • Author(s)
      Filip Husnik
    • Organizer
      The 4th Asian Congress of Protistology
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2021-04-28   Modified: 2024-01-30  

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