| Project/Area Number |
21K15094
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 44010:Cell biology-related
|
|---|
| Research Institution | Okinawa Institute of Science and Technology Graduate University |
|---|
Principal Investigator |
Husnik Filip 沖縄科学技術大学院大学, 進化・細胞・共生の生物学ユニット, 准教授 (30886130)
|
|---|
| Project Period (FY) |
2021-04-01 – 2023-03-31
|
| Project Status |
Completed (Fiscal Year 2022)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | symbiosis / metabolomics / proteomics / FIB-SEM / uCT / SBF-SEM / genomics / FISH / bacteria / insect / organelle / 3D microscopy / insects / immunohistochemistry / 3D imaging / protein import / evolution |
|---|
| Outline of Research at the Start |
Long-term endosymbiosis results in massive dependence on the host. Mitochondria and plastids, as the oldest known endosymbionts, are well-recognized to rely on import of hundreds of proteins from the host cytoplasm. However, recent results, including my preliminary data, show that proteins can be (by an unknown mechanism) also imported into endosymbionts with highly reduced genomes, blurring the line between endosymbionts and organelles. A fundamental question I will target here is if the protein import can be as massive as in organelles.
|
| Outline of Final Research Achievements |
In the frame of the project, I have focused on host-symbiont integration in a unique bacterium-within-bacterium-within-eukaryote symbiosis found in mealybugs. Via the combination of omics (genomics, transcriptomics, proteomics, and metabolomics) with 3D imaging (uCT, confocal FISH imaging, SBF-SEM, FIB-SEM), we managed to understand which genes, proteins, and metabolites are localized in the system and how they drive the tripartite interaction. Integrating data from all these advanced methods is unprecedented for a single symbiotic species and allows us to draw strong conclusions about host-symbiont integration (e.g. cell division coordination and protein import) in highly interdependent, organelle-like, symbioses.
|
| Academic Significance and Societal Importance of the Research Achievements |
Understanding how highly integrated symbioses function is essential(and relates to human health) since one organelle in our cells, the mitochondrion, has originated from a symbiotic bacterium.
|
