Reconstituion in vitro of primate germ cell development
Project/Area Number |
21K15112
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 44020:Developmental biology-related
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Research Institution | Institute of Physical and Chemical Research (2022) Kyushu University (2021) |
Principal Investigator |
Yoshimatsu Sho 国立研究開発法人理化学研究所, 脳神経科学研究センター, 客員研究員 (00882713)
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Project Period (FY) |
2021-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 生殖細胞 / マーモセット / 霊長類 / シングルセル / 多能性幹細胞 / 卵子 / ゲノム編集 / レポーター / 再構築 |
Outline of Research at the Start |
生殖細胞系譜は次世代に遺伝情報を伝達し、その系譜分化の機構究明は進化生物学・発生学上の重要課題とされてきた。近年、マウス多能性幹細胞から生殖系列細胞を誘導し産仔形成能をもつ機能的配偶子が作出されたという成果は、「多能性幹細胞からのin vitro配偶子形成」という革新的生殖技術に繋がり、ヒト細胞についてもin vitro配偶子形成研究の新領域を拓いた。しかし生殖医療への応用を踏まえた場合、実験的検証を可能とする非ヒト霊長類を用いた培養系の開発が求められる。そこで本研究では小型霊長類のマーモセットを対象として、多能性幹細胞から配偶子分化をin vitroで再現する培養系の確立を最終目標とする。
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Outline of Final Research Achievements |
In this study, single cell analysis of germline cells from marmosets allowed us to identify primate-specific gene expression profiles, a major step toward future reconstruction in culture. Also, we generated reporter embryonic stem cell lines of marmosets harboring gonadal somatic cell-reporters. It would promote further studies for in vitro reconstitution of functional gametes from marmosets.
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Academic Significance and Societal Importance of the Research Achievements |
本研究の継続よって、生殖細胞の再構築を検討する上で、近年深刻になっている少子高齢化や、不妊治療に対する根本的な解決策を創出できることがきたいされる。
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Report
(3 results)
Research Products
(26 results)
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[Journal Article] Critical roles of FGF, RA, and WNT signaling in the development of human otic placode and subsequent lineages in a dish2022
Author(s)
Saeki, T., Yoshimatsu, S., Ishikawa, M., Shin, JW., Hon, C-C., Shibata, S., Hosoya, M., Saegusa, C., Ogawa, K., Fujioka, M., Okano, H.
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Journal Title
Regen Ther
Volume: 20
Pages: 165-186
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Multimodal analyses of a non-human primate model harboring mutant amyloid precursor protein transgenes driven by the human EF1α promoter.2022
Author(s)
Yoshimatsu S, Seki F, Okahara J, Watanabe H, Sasaguri H, Haga Y, Hata JI, Sanosaka T, Inoue T, Mineshige T, Lee CY, Shinohara H, Kurotaki Y, Komaki Y, Kishi N, Murayama AY, Nagai Y, Minamimoto T, Yamamoto M, Nakajima M, Zhou Z, Nemoto A, Sato T, Ikeuchi T, Sahara N, Morimoto S, Shiozawa S, Saido TC, Sasaki E, Okano H
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Journal Title
Neuroscience Research
Volume: 185
Pages: 49-61
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Attempts for deriving extended pluripotent stem cells from common marmoset embryonic stem cells2022
Author(s)
Yoshimatsu S, Nakajima M, Sonn I, Natsume R, Sakimura K, Nakatsukasa E, Sasaoka T, Nakamura M, Serizawa T, Sato T, Sasaki E, Deng H, Okano H
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Journal Title
Genes to Cells
Volume: 28(2)
Issue: 2
Pages: 156-169
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species.2021
Author(s)
Yoshimatsu S, Nakajima M, Iguchi A, Sanosaka T, Sato T, Nakamura M, Nakajima R, Arai E, Ishikawa M, Imaizumi K, Watanabe H, Okahara J, Noce T, Takeda Y, Sasaki E, Behr R, Edamura K, Shiozawa S, Okano H.
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Journal Title
Stem Cell Reports
Volume: 16
Issue: 4
Pages: 754-770
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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