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Relaxation of limitation to photosynthesis by an electrochemical technique

Research Project

Project/Area Number 21K15122
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionKwansei Gakuin University

Principal Investigator

Shimakawa Ginga  関西学院大学, 生命環境学部, 助教 (60853885)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords光合成 / 細胞電流 / 酸化的ペントースリン酸経路 / シアノバクテリア / レドックス / 電気化学
Outline of Research at the Start

生物工学的な光合成反応の制御や改変は、太陽エネルギーを利用した高付加価値の物質生産を実現する上で避けては通れない課題である。これまで遺伝子改変を主として光合成の改変を目指した研究が盛んに行われた中で、光合成生物そのものが有する光合成の制御メカニズムはしばしば軽視されてきた。そこで本研究では、外部電極およびメディエーターを用いた電気化学的手法によって電子伝達系のレドックスに干渉し、RISEによる電子伝達反応の抑制を解除、再誘導することを試みる。その中で申請者は本実験技術の開発およびそれを用いた光合成物質生産の向上、RISEによる活性酸素生成抑制メカニズムの解明に取り組む。

Outline of Final Research Achievements

During the term of the research, we clarified that the oxidative pentose phosphate pathway (OPPP) in cyanobacteria affects the magnitude of cellular currents through plastoquinone reduction, and worked to quantify its physiological activity. It was found to be about 40%. In addition, while I was partly involved in research that clarified the effect of the extracellular membrane on extracellular electron transfer in cyanobacteria, we presumed that the exfoliation of the extracellular membrane could work effectively in this project as well. At the end of this year, I wrote a review article on cyanobacterial electron transfer reactions, including photosynthesis and respiratory electron transfer, and reconsidered the important findings in aiming to lift the limiter of photosynthesis.

Academic Significance and Societal Importance of the Research Achievements

光合成電子伝達を抑制するリミッターとして、これまで関連分野における多くの研究ではチラコイド膜内外におけるプロトン勾配依存的な制御メカニズム (PREG) が注目されてきたが、還元により誘導される抑制(RISE)の生理機能や分子メカニズムについては未だ不明な点が多く、本研究で得られた知見は従来の視点とは異なる新たなコンセプトで光合成制御を操作する発想へと繋がるものである。
光合成の改変を目的とした研究には、主として遺伝子操作を用いることで光合成能力や環境ストレス耐性の向上を目指したものが多い。本研究で行った野生株ベースでの電気化学的なリミッター解除による光合成の促進は新奇アプローチといえる。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (9 results)

All 2023 2022 2021

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) (of which Int'l Joint Research: 1 results,  Invited: 4 results)

  • [Journal Article] Electron transport in cyanobacterial thylakoid membranes: Are cyanobacteria simple models for photosynthetic organisms?2023

    • Author(s)
      Shimakawa G
    • Journal Title

      Journal of Experimental Botany

      Volume: - Issue: 12 Pages: 3476-3487

    • DOI

      10.1093/jxb/erad118

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Order-of-magnitude enhancement in photocurrent generation of Synechocystis sp. PCC 6803 by outer membrane deprivation2022

    • Author(s)
      Kusama S, Kojima S, Kimura K, Shimakawa G, Miyake C, Tanaka K, Okumura Y, Nakanishi S
    • Journal Title

      Nature Communications

      Volume: 13 Issue: 1

    • DOI

      10.1038/s41467-022-30764-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dissection of respiratory and cyclic electron transport in Synechocystis sp. PCC 68032022

    • Author(s)
      Kusama S, Miyake C, Nakanishi S, Shimakawa G
    • Journal Title

      Journal of Plant Research

      Volume: 135 Issue: 4 Pages: 555-564

    • DOI

      10.1007/s10265-022-01401-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] NADPH production in dark stages is critical for cyanobacterial photocurrent generation: a study using mutants deficient in oxidative pentose phosphate pathway2022

    • Author(s)
      Hatano Jiro、Kusama Shoko、Tanaka Kenya、Kohara Ayaka、Miyake Chikahiro、Nakanishi Shuji、Shimakawa Ginga
    • Journal Title

      Photosynthesis Research

      Volume: - Issue: 1-2 Pages: 113-120

    • DOI

      10.1007/s11120-022-00903-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] O2 photoreduction mediated by flavodiiron proteins2022

    • Author(s)
      Shimakawa G
    • Organizer
      French Photosynthesis Society
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] なぜ褐虫藻はサンゴと共生するのか?2022

    • Author(s)
      嶋川銀河
    • Organizer
      サンゴ礁生態系におけるレドックスエコバイオロジー
    • Related Report
      2022 Annual Research Report
  • [Presentation] Evolution and diversity of photosynthetic organisms based on the strategy for P700 oxidation2022

    • Author(s)
      嶋川銀河
    • Organizer
      日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 藍藻チラコイド膜の電子伝達2021

    • Author(s)
      嶋川銀河
    • Organizer
      藍藻ゲノム交流会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] “生きている光合成”から学ぶこと~光合成の反応制御と活性酸素生成抑制システム~2021

    • Author(s)
      嶋川銀河
    • Organizer
      電気化学秋季大会
    • Related Report
      2021 Research-status Report
    • Invited

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Published: 2021-04-28   Modified: 2024-01-30  

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