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Identification of the sexually antagonistic gene responsible for diversified black nuptial coloration in medaka fishes

Research Project

Project/Area Number 21K15143
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionTohoku University

Principal Investigator

Ansai Satoshi  東北大学, 生命科学研究科, 助教 (20779467)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords性的葛藤 / 性淘汰 / 性染色体 / 性的二型 / メダカ / 古代湖 / 種分化 / ゲノム解析 / 婚姻色 / ゲノム編集 / 原因遺伝子 / 性選択 / メラニン
Outline of Research at the Start

本研究では、黒色婚姻色パターンと性染色体の急速な種間変異を有するメダカ科魚類ポソ湖固有種群をモデルとして、性的葛藤遺伝子の機能解析や、その解消に関わる詳細な遺伝機構の解明に取り組む。まず、遺伝学的解析や比較トランスクリプトーム解析、性染色体上の候補遺伝子解析の3つのアプローチをとり、黒色婚姻色の種差・性差の原因遺伝子を同定する。ゲノム編集を用いた遺伝子改変により、同定した候補遺伝子が婚姻色の性差や種間差を生み出す上での役割を解明する。また、ゲノム改変個体を用いた配偶行動実験や発色コストの解析から、雌雄の適応度に及ぼす影響を定量する。

Outline of Final Research Achievements

To identify the sexual conflict gene responsible for the diversification of sexual dimorphism and to elucidate the detailed genetic mechanism involved in its evolution, we analyzed the causative gene for the diversified black nuptial coloration and sex chromosome turnover among three species of medaka fishes endemic to Lake Poso, Indonesia. QTL analysis using interspecific hybrid families revealed one locus associated with black nuptial color. Whole genome sequencing also revealed sex chromosomes in two of the three species. These results indicate that the sex chromosomes differ among the three species and that the locus related to black nuptial color is located on an autosomal chromosome.

Academic Significance and Societal Importance of the Research Achievements

本研究により、ポソ湖固有の3種のメダカ科魚類は、異なる性染色体を有することが明らかとなった。このことは、先行研究により示唆されている、種分化における性染色体転換の重要性を強調する研究成果である。さらなる解析により、黒色婚姻色の原因遺伝子や性決定遺伝子の実体が解明できれば、性染色体転換と種分化がどのように関連しているのか、その詳細を様々なレベルで解明することが可能となる。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 5 results)

  • [Int'l Joint Research] サムラトゥランギ大学(インドネシア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] サムラトゥランギ大学(インドネシア)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Diversity of sex chromosomes in Sulawesian medaka fishes2022

    • Author(s)
      Ansai Satoshi、Montenegro Javier、Masengi Kawilarang W. A.、Nagano Atsushi J.、Yamahira Kazunori、Kitano Jun
    • Journal Title

      Journal of Evolutionary Biology

      Volume: 35 Issue: 12 Pages: 1751-1764

    • DOI

      10.1111/jeb.14076

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Speciation and adaptation research meets genome editing2022

    • Author(s)
      Ansai Satoshi、Kitano Jun
    • Journal Title

      Philosophical Transactions of the Royal Society B: Biological Sciences

      Volume: 377 Issue: 1855 Pages: 20200516-20200516

    • DOI

      10.1098/rstb.2020.0516

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Presentation] The evolutionary basis of diversified sexually selected traits in Indonesian medaka fishes2022

    • Author(s)
      Satoshi Ansai
    • Organizer
      日本発生生物学会第55回大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] メダカにおける遺伝子ノックイン・染色体編集技術の開発とその利用2022

    • Author(s)
      安齋 賢
    • Organizer
      日本動物学会 第93回 早稲田大会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Toward understanding the genetic basis of phenotypic diversities of Sulawesian and Japanese medaka fishes2022

    • Author(s)
      Satoshi Ansai
    • Organizer
      EMBL-EBI Training - Using fish models to explore the impact of Gene by Environment (GxE) 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] インドネシアのメダカ科固有種群における性的二型多様化の分子機構2021

    • Author(s)
      安齋 賢, 山平 寿智, 北野 潤
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Sex chromosome turnover in Sulawesian medaka fishes2021

    • Author(s)
      Satoshi Ansai, Kawilarang W. A. Masengi, Kazunori Yamahira, Jun Kitano
    • Organizer
      ESEB Satellite Symposia 2021 - The remarkable diversity in the rate and mechanisms of sex chromosome evolution
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Genome editing reveals fitness effects of a gene for sexual dichromatism in Sulawesian fish2021

    • Author(s)
      Satoshi Ansai
    • Organizer
      Japan-Norway Symposium on Fish Endocrinology and Genomics
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2021-04-28   Modified: 2024-01-30  

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