| Project/Area Number |
21K15187
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46010:Neuroscience-general-related
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
Daun Kenny 滋賀医科大学, 医学部, 特任助教 (40896510)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | Postnatal amygdala / Neurogenesis / Early-life stress / Lineage tracing / Postnatal Amygdala / early-life stress |
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| Outline of Research at the Start |
We will utilize the maternal and social deprivation paradigms to elucidate the origins and cell-type specificity when exposing to the early-life stress by (1) a lineage tracing system carrying the inducible Cre-recombinase and (2) the fate analysis using GFP expressing lentivirus.
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| Outline of Final Research Achievements |
Neurogenesis in the postnatal amygdala remains unknown. We have previously found that neurogenesis in the amygdala was altered by environmental stimuli due to maternal and social deprivation (MSD) inflicted after birth. However, the details of its subtypes and origin are not clear. In this study, to infer the fate and origin of neonatal neurons in the amygdala, we performed amygdala lineage analysis of GFP-labelled neural stem cells (NSCs) after MSD, as well as single-cell RNA sequencing analysis and behavioral analysis to examine the differentiation trajectories of amygdalar neurogenesis and the physiological role of newborn neurons.
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| Academic Significance and Societal Importance of the Research Achievements |
As newborn cells, particularly during the postnatal period, are context-sensitive, they can be manipulated by environmental stimuli. Understanding the contributions of amygdalar neurogenesis after early-life stress will provide new insight into fundamental aspects of brain plasticity.
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