Discovery and characterization of a pressure sensation and transduction (PREST) mechanism in the presynaptic terminal in the CNS.
| Project/Area Number |
21K15203
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UCAR HASAN 東京大学, ニューロインテリジェンス国際研究機構, 特任助教 (50748423)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Discontinued (Fiscal Year 2022)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2025: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2024: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | Presynaptic terminal / mechanical stimulation / mechanobiology / PREST / Release probabilty / Optogenetics / iGluSnFr / FRET / Spine enlargement / Mechanosensation / Synaptic plasticity / SNARE assembly / Glutamate release |
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| Outline of Research at the Start |
Using 2-photon and STED-microscopy I will study the molecular mechanisms of our novel finding of the pressure sensation and transduction (PREST) mechanism in the presynaptic terminal in the brain.
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| Outline of Annual Research Achievements |
We announced mechanical transmission and PREST in CNS in 2021 (Ucar et al., Nature, 2021).
I am studying the underlying mechanisms of PREST and we published a review article to underline its physiological importance for brain function and memory.
Recently I found that mechanical stimulation activates intracellular signaling pathways which lead to synaptic vesicle regulation during PREST. Here, synaptic vesicle clusters are mobilized upon mechanical stimulation and this is estimated to be regulated by a kinase pathway that I am working on.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
I was able to conlude that presynaptic terminal responds to mechanical stimulation and made a publication in Nature (Ucar et al. Nature 2021) and described PREST (pressure sensation and transduction) mechanism. Now I am studying underlying mechanisms for which I have promising data.
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| Strategy for Future Research Activity |
Now I am studying underlying mechanisms of PREST for which I have promising data. I will continue testing new molecular probes to understand the involvement of molecules for the regulation and function of PREST and their regulation by the intracellular kinases by using two-photon microscopy.
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Report
(2 results)
Research Products
(8 results)
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[Presentation] Mechanosensation and PREST in the presynaptic terminals2022
Author(s)
Hasan Ucar, Satoshi Watanabe, Jun Noguchi, Sho Yagishita, Yuichiro Morimoto, Noriko Takahashi, Haruo Kasai
Organizer
The 45th Annual Meeting of the Japan Neuroscience Society. The 65th Annual Meeting of the Japanese Society for Neurochemistry The 32nd Annual Conference of the Japanese Neural Network Society
Related Report
Int'l Joint Research / Invited
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