| Project/Area Number |
21K15203
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | The University of Tokyo |
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Principal Investigator |
UCAR HASAN 東京大学, ニューロインテリジェンス国際研究機構, 特任助教 (50748423)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Discontinued (Fiscal Year 2023)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2025: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2024: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | presynaptic plasticity / PREST / mechanical transmission / SV mobility / Presynaptic terminal / mechanical stimulation / mechanobiology / Release probabilty / Optogenetics / iGluSnFr / FRET / Spine enlargement / Mechanosensation / Synaptic plasticity / SNARE assembly / Glutamate release |
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| Outline of Research at the Start |
Using 2-photon and STED-microscopy I will study the molecular mechanisms of our novel finding of the pressure sensation and transduction (PREST) mechanism in the presynaptic terminal in the brain.
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| Outline of Annual Research Achievements |
I discovered a new mechanism of synaptic vesicle(SV) mobility upon mechanical stimulation of the bouton. 1)Pushing causes dispersion of the SVs in bouton 2) Pushing enhances exocytosis in high-frequency stimulation 3)Synapsin, an SV binding protein, is also dispersed upon pushing 4)A dominant negative synapsin prevents SV dispersion, the Pr-increase and SNARE assembly that normally occurs after pushing 5)Inhibition of ERK1/2 also prevents SV dispersion, the Pr-increase and SNARE assembly that normally occurs after pushing. In summary, mobilization of SVs could enhance SV replenishment (a functional connection between 1 and 2 is to be established). These effects could be mediated by ERK phosphorylation on synapsin proteins (a functional connection between 3 and 4 is to be established).
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