| Project/Area Number |
21K15214
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 46030:Function of nervous system-related
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Sekulic Vladislav 国立研究開発法人理化学研究所, 脳神経科学研究センター, 特別研究員 (60865492)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Granted (Fiscal Year 2022)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | hippocampus / memory / engram / interneuron / calcium imaging / physiology / memory encoding / electrophysiology / imaging / microcircuits |
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| Outline of Research at the Start |
This research aims to determine how region-specific differences in memory formation within the hippocampus are governed by differences in inhibitory interneuron function. This work will be critical in determining how manipulations of interneurons can help precisely modulate memory acquisition.
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| Outline of Annual Research Achievements |
As reported in the FY2021 F-7-1 year end report, the main dataset of calcium recordings were completed more rapidly than expected, allowing time to expand the dataset. This was preformed in FY2022. I recorded pilot mice with dual imaging of immediate early gene (memory labelling) neurons as well as dynamic activity of all neurons to verify the system, and also performed an additional dataset of recordings of mice to verify the mechanism of action of the previous results. In particular, I recorded directly from the cells implicated in my earlier results using fluorescence indicators in live behaving mice to show that the effect of the chemogenic drug to shut down or activate those neurons actually worked as expected. This dataset can also be analyzed for additional insights as to how the memory is enhanced or disrupted in the behavioral tasks performed.
I furthermore performed significant data analysis of the previous main dataset and developed a novel algorithm for detecting “place field” information from the recorded neurons in an open field environment using statistical measures and machine learning tools like varational Bayesian Gaussian mixture models.
I was also able to complete a new dataset on performing tracing studies on the brain regions that provide inputs to the part of hippocampus implicated in this study, to show that the manipulation performed earlier results in changes in IEG-mediated synaptic contacts consistent with the pattern of manipulation performed, to verify the long-term effects of the memory changes.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
As outlined in the Summary of Research Achievements section above, the progress in FY2022, building on the success of the progress in FY2021, was able to significantly advance the state of the research beyond the initially planned calcium imaging dataset and is now also pursuing several additional followup avenues of investigation to provide a complete picture of the implications of the impact of the research questions being examined.
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| Strategy for Future Research Activity |
During the last few months of work on this project, the dataset analysis will be the primary focus and promoting the research at local and international meetings. I plan on presenting the research at the annual Japanese Neuroscience Society in Sendai in August 2023. I will also present the research at the Society of Neuroscience international meeting in Washington, D.C. in November 2023.
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