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Genetic Aberrations Defining Clinicopathological Features in DLBCL Subtypes

Research Project

Project/Area Number 21K15405
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 49020:Human pathology-related
Research InstitutionNagoya City University

Principal Investigator

Fujii Keiichiro  名古屋市立大学, 医薬学総合研究院(医学), 助教 (50896545)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords血液腫瘍 / 悪性リンパ腫 / びまん性大細胞型B細胞リンパ腫 / DLBCL亜型 / 亜型
Outline of Research at the Start

びまん性大細胞型B細胞リンパ腫(DLBCL)には多くの亜型が存在する。血管内大細胞型B細胞リンパ腫(IVL)は稀なDLBCL亜型で、検体中の腫瘍細胞数が少数のため遺伝子解析が難しい。本研究の目的は①申請者らが独自に開発したパラフィン組織を用いて細胞レベルで形態、蛋白発現、遺伝子解析ができる手法によりIVLの遺伝子異常に依存した病態を明らかにする。②DLBCL亜型の症例を対象に、リンパ腫細胞を次世代シーケンサおよびFISH法を用いて、網羅的遺伝子プロファイリングを行う。これにより、各亜型の臨床的特徴と遺伝子異常の関係を突き止め、全体像の把握につなげ、個別化治療検討の足掛かりにしたい。

Outline of Final Research Achievements

In fifteen IVL cases, translocations of BCL2, BCL6, and MYC genes, and amplification of PD-L1 gene were analyzed using a FICTION-WSI method. None of the cases had translocations, but some showed aberrant FISH signal gains. This observation may be related to IVL lymphomagenesis, but further investigations may be necessary. We plan to continue our research even after the research period ends.
To gain a better understanding of DLBCL and to optimize individualized prognosis assessment, we developed nomograms to predict the survival of DLBCL patients. We also included MYD88 and CD79B mutations in the prognostic model.

Academic Significance and Societal Importance of the Research Achievements

DLBCL患者個々の予後予測をするための新たなモデル(ノモグラム)を開発した。さらにDLBCLの予後不良に関連する遺伝子異常をIPI因子と融合した予後予測モデル(ノモグラム)を開発した。これらのノモグラムは臨床現場において患者個々の予後評価に役立ち、治療方針やフォローアップ期間などの決定に貢献する可能性がある。また遺伝子異常を既存のIPI因子と融合させた本研究のアプローチは画期的であり、本研究はリンパ腫分野における予後予測モデル開発の基盤になる可能性がある。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (7 results)

All 2024 2022

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Nomogram for predicting survival of patients with diffuse large B-cell lymphoma2024

    • Author(s)
      Fujii Keiichiro、Inagaki Atsushi、Masaki Ayako、Sugiura Mariko、Suzuki Tomotaka、Ishida Takashi、Kusumoto Shigeru、Iida Shinsuke、Inagaki Hiroshi
    • Journal Title

      Annals of Hematology

      Volume: - Issue: 6 Pages: 2041-2050

    • DOI

      10.1007/s00277-024-05669-0

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescent periodic acid Schiff-like staining combined with standard cytologic staining of the same cytologic specimen may facilitate cytopathologic diagnosis.2024

    • Author(s)
      Hachisuka D, Nakano S, Takase H, Sugiyama M, Sugiura M, Fujii K, Masaki A, Murase T, Inagaki H.
    • Journal Title

      Diagn Cytopathol. 2024 Mar;52(3):139-144.

      Volume: 52 Issue: 3 Pages: 139-144

    • DOI

      10.1002/dc.25256

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 示指に発生した血管内乳頭状内皮過形成の1例2024

    • Author(s)
      杉山 美緒、杉浦 真理子、中野 さつき、藤井 慶一郎、正木 彩子、村瀬 貴幸、稲垣 宏、山田 聡
    • Journal Title

      診断病理

      Volume: 41 Pages: 64-68

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 7-Amino-4-methylcoumarin as a fluorescent substitute for Schiff’s reagent: a new method that can be combined with hemalum and eosin staining on the same tissue section2022

    • Author(s)
      Takase Hiroshi、Murase Takayuki、Hachisuka Daisuke、Sakamoto Yuma、Sugiura Mariko、Nakano Satsuki、Fujii Keiichiro、Masaki Ayako、Inagaki Hiroshi
    • Journal Title

      Biotechnic & Histochemistry

      Volume: 98 Issue: 1 Pages: 54-61

    • DOI

      10.1080/10520295.2022.2101144

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Pleomorphic adenoma: detection of PLAG1 rearrangement?positive tumor components using whole-slide fluorescence in situ hybridization2022

    • Author(s)
      Murase Takayuki、Ishibashi Kenichiro、Okumura Yoshihide、Ueda Kaori、Nakano Satsuki、Fujii Keiichiro、Masaki Ayako、Nagao Toru、Inagaki Hiroshi
    • Journal Title

      Human Pathology

      Volume: 120 Pages: 26-34

    • DOI

      10.1016/j.humpath.2021.11.010

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] びまん性大細胞型B細胞リンパ腫患者の予後を予測するノモグラム2024

    • Author(s)
      藤井慶一郎、稲垣淳、正木彩子、杉浦真理子、鈴木智貴、石田高司、楠本茂、飯田真介、稲垣宏
    • Organizer
      第113回日本病理学会総会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 蛍光PAS染色を用いた細胞診断の有用性について2024

    • Author(s)
      蜂須賀大輔、中野さつき、高瀬弘嗣、杉山美緒、杉浦真理子、藤井慶一郎、正木彩子、村瀬貴幸、稲垣宏
    • Organizer
      第113回日本病理学会総会
    • Related Report
      2023 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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