Identification of genetic basis of virulence determinants of emerging Bacillus cereus ST1420 using silkworm model

Research Project

Project/Area Number	21K15430
Research Category	Grant-in-Aid for Early-Career Scientists
Allocation Type	Multi-year Fund
Review Section	Basic Section 49050:Bacteriology-related
Research Institution	Hokkaido University
Principal Investigator	PAUDEL ATMIKA 北海道大学, 人獣共通感染症国際共同研究所, 特任助教 (80832774)
Project Period (FY)	2021-04-01 – 2024-03-31
Project Status	Granted (Fiscal Year 2021)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2022: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	Bacillus cereus / ST1420 / pathogenecity / silkworm / virulence
Outline of Research at the Start	In our laboratory, we recently identified B. cereus with a novel sequence type ST1420 to be dominant in B. cereus outbreaks in 3 geographically distant large hospitals in Japan. This research plans to understand the genetic basis of pathogenicity and virulence determinants of B. cereus ST1420 by constructing transposon-mutant library of the bacteria and using silkworm (Bombyx mori) model.