| Project/Area Number |
21K16203
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 53050:Dermatology-related
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| Research Institution | University of Tsukuba |
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Principal Investigator |
周 思奇 筑波大学, 生命環境系, 研究員 (60869676)
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| Project Period (FY) |
2021-04-01 – 2023-03-31
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| Project Status |
Discontinued (Fiscal Year 2022)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | iPS cell / 3D organoid / hair follicles / Hair development / organoid / hair loss |
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| Outline of Research at the Start |
Alopecia (hair loss) is a common dermatological condition that bothers people and causes psychosocial aspects of stress. There are many triggers for hair loss, such as androgens, stresses, or anti-cancer drugs. However, there is no appropriate model in vitro for studying hair loss. Therefore, I aim to use iPS cells to establish a novel hair follicle development model in vitro. by the low-cell-adhesion U-bottom plate formed 3D organoid. Furthermore, a model of hair loss induced by different factors (such as androgen, anti-cancer reagents, stress stimulus, etc.) also can be established.
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| Outline of Annual Research Achievements |
In this project, we aim to investigate the hair growth and loss by inducing iPS cell differentiation to form a 3D hair follicle tissue model in vitro.
In the first year, we successfully established the iPS culture system in our lab and maintained the ability of pluripotent differentiation after 10 passages of culture. From the second year, we firstly induced iPS cells differentiation into specific cells associated with hair follicles: keratinocytes, fibroblasts, and dermal papilla. Next, we prepared U-shaped 96-well dishes to induce iPS cells forming spheroids. Unfortunately, contrary to our expectations, we found that iPS cells didn't spontaneously aggregate together to form spheroids like other cells.
Therefore, we decided to use Matrigel Matrix, an extracellular matrix that has been used in many studies to assist in cell formation of organoids. After mixing iPS cells with Matrigel, the cells were randomly immobilized. After 3 days with culture medium, the formation of organoids was evident, and a significant enlargement was observed at day 5. Morphological changes in cell differentiation were also observed after the addition of growth factors. These results suggest that Matrigel Matrix can provide a suitable 3D environment to help cells form structures similar to real tissues and can be used for cell differentiation.
Unfortunately the investigator is unable to complete the further experiments due to a position change. While, these results still provide valuable information and novel method to study the mechanisms of histogenesis and cell differentiation in the future.
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