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Role of gap junctions in cholinergic amacrine cells on visual information processing maturation

Research Project

Project/Area Number 21K16910
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 56060:Ophthalmology-related
Research InstitutionTokyo Women's Medical University (2022)
Nippon Medical School (2021)

Principal Investigator

Maruyama Takuma  東京女子医科大学, 医学部, 助教 (90838103)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2022: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsコリン作動性アマクリン細胞 / スターバーストアマクリン細胞 / 網膜 / ギャップ結合 / 生後発達 / コネキシン / ドーパミン受容体 / 視覚経験 / パッチクランプ
Outline of Research at the Start

網膜神経回路の成熟には、コリン作動性アマクリン細胞由来のretinal waveが関与する。Retinal waveの生成には、ギャップ結合やアセチルコリン入力が関与しているが、成熟動物のコリン作動性アマクリン細胞にはギャップ結合が存在しない。本研究では、生後発達期のコリン作動性アマクリン細胞に存在するギャップ結合の分子実体とその消滅メカニズムを明らかにすることで、retinal wave生成におけるコリン作動性アマクリン細胞のギャップ結合の役割を明らかにしようとするものである。

Outline of Final Research Achievements

The purpose of this study was to determine whether cholinergic amacrine cells, which play an important role in the maturation of neural circuits in the retina, form gap junctions during the developmental stages of the mouse retina. Using immunohistochemical, molecular biological and electrophysiological analyses, I found that cholinergic amacrine cells form gap junctions with neighboring heterogeneous cells before the eye opens.

Academic Significance and Societal Importance of the Research Achievements

本研究は、生後発達期の網膜におけるコリン作動性アマクリン細胞の機能の理解に貢献するとともに、網膜神経回路の成熟化におけるコリン作動性アマクリン細胞の役割の解明につながることが期待される。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 2021

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (6 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Starburst amacrine cells form gap junctions in the early postnatal stage of the mouse retina2023

    • Author(s)
      Maruyama Takuma、Ishii Toshiyuki、Kaneda Makoto
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 17 Pages: 00-00

    • DOI

      10.3389/fncel.2023.1173579

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] P2X2 receptors supply extracellular choline as a substrate for acetylcholine synthesis2022

    • Author(s)
      Maruyama Takuma、Mano Asuka、Ishii Toshiyuki、Kakinuma Yoshihiko、Kaneda Makoto
    • Journal Title

      FEBS Open Bio

      Volume: 12 Issue: 1 Pages: 250-257

    • DOI

      10.1002/2211-5463.13332

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Starburst amacrine cells form gap junctions in the early postnatal stage of the mouse retina2023

    • Author(s)
      Toshiyuki Ishii, Takuma Maruyama, Makoto Kaneda
    • Organizer
      第100回日本生理学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Starburst amacrine cells form gap junctions in early postnatal stage of the mouse retina2022

    • Author(s)
      Toshiyuki Ishii, Takuma Maruyama, Makoto Kaneda
    • Organizer
      Neuroscience2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Starburst amacrine cells in the early postnatal development form gap junctions2022

    • Author(s)
      Takuma Maruyama, Toshiyuki Ishii, Sumiko Usui, Masumi Shimizu, Makoto Kaneda
    • Organizer
      第99回日本生理学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Starburst amacrine cells in the early postnatal development form gap junctions2022

    • Author(s)
      Takuma Maruyama, Toshiyuki Ishii, Sumiko Usui, Masumi Shimizu, Makoto Kaneda
    • Organizer
      第99回 日本生理学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] The role of P2X2 receptor for Acetylcholine synthesis pathway2021

    • Author(s)
      Takuma Maruyama, Asuka Mano, Toshiyuki Ishii, Yoshihiko Kakinuma, Makoto Kaneda
    • Organizer
      The Society of Neuroscience Meeting 2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] P2X2 receptor-mediated novel acetylcholine synthesis pathway2021

    • Author(s)
      Takuma Maruyama, Toshiyuki Ishii, Asuka Mano, Yoshihiko Kakinuma, Makoto Kaneda
    • Organizer
      第44回日本神経科学大会
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2024-01-30  

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