An idea of regulatory science-based bone regeneration product for critical bone defect healing

• Project/Area Number: 21K16962
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Tohoku University
• Principal Investigator: Venkataiah VenkataSuresh 東北大学, 大学病院, 講師 (60898477)
• Project Period (FY): 2021-04-01 – 2024-03-31
• Project Status: Discontinued (Fiscal Year 2023)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2024: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2023: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: Regenerated Bone / Nanoindentation / Implant Therapy / Functional Bone / Micro-CT analysis / Calvaria osteoblasts / Polylactic acid scaffold / Critical bone defect / Bone tissue engineering / Bone product

Outline of Research at the Start

In the proposed study, we will establish a method to develop bone regeneration product that will enhance healing of various bone defects.
Our main goal is to develop a method to prepare a regulatory science-based quality-controlled bone regeneration product with enhanced bone regeneration ability which can used for horizontal bone loss caused of severe periodontitis.

Outline of Annual Research Achievements

After quantitatively assessed new bone in mice jaw bone defects, we investigated mechanical and functional properties of regenerated bone. The Nanoindentation tests showed cytrans group exhibit higher tissue hardness and elastic modulus, followed by 3DPL4, MCOB-3DPL4, and empty groups. To assess functional properties, mini-implants placed in defect area. CT analysis showed lack of complete osseointegration of the implants across all groups. HE staining showed good osseointegration in the MCOB-3DPL4 and cytrans groups, partial osseointegration in the 3DPL4 and empty defect groups

Research Products

• [Journal Article] Clinical Applications of Cell-Scaffold Constructs for Bone Regeneration Therapy (2021)
  • Author(s): Venkataiah Venkata Suresh、Yahata Yoshio、Kitagawa Akira、Inagaki Masahiko、Kakiuchi Yusuke、Nakano Masato、Suzuki Shigeto、Handa Keisuke、Saito Masahiro
  • Journal Title: Cells Volume: 10 Issue: 10 Pages: 2687-2687
  • DOI: 10.3390/cells10102687
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Development of Bone Regeneration Product using Immature osteoblasts and three-dimensional polymer scaffold. (2022)
  • Author(s): Venkata Suresh Venkataiah, Shigeto Suzuki, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Mary M. Njuguna, Risako Nozawa, Yusuke Kakiuchi, Masato Nakano, Keisuke Handa, Masahiro Yamada, Hiroshi Egusa and Masahiro Saito
  • Organizer: The 2022 Autumn Annual Meeting of JSCD (154th) & JKDC academic conference
• [Presentation] Development of bone tissue engineering technology using immature osteoblasts and three-dimensional polymer scaffold (2022)
  • Author(s): Venkata Suresh Venkataiah, Shigeto Suzuki, Yoshio Yahata, Akira Kitagawa, Masahiko Inagaki, Mary M. Njuguna, Risako Nozawa, Yusuke Kakiuchi, Masato Nakano, Keisuke Handa, Masahiro Yamada, Hiroshi Egusa and Masahiro Saito
  • Organizer: The International Oral Health Symposium 2022.