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Application of multifunctional membrane using enamel protein for periodontal tissue regeneration

Research Project

Project/Area Number 21K16986
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57030:Conservative dentistry-related
Research InstitutionShowa University (2023)
Tokyo Medical and Dental University (2021-2022)

Principal Investigator

Yuichi Ikeda  昭和大学, 歯学部, 兼任講師 (30736179)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsエナメルマトリクスタンパク質 / 歯周組織再生 / エナメル蛋白質 / メンブレン
Outline of Research at the Start

これまでAMTN、ODAMというタンパク質を用いて①歯と骨に接着し、②膜表面が生体内で石灰化し、③骨芽細胞の石灰化を誘導し、④膜表面に歯肉結合組織が接着する性質をもつ画期的な再生治療用メンブレンを開発してきました。このメンブレンはマウス頭蓋骨欠損モデルにおいて有意な骨治癒の増加を得られることを確認しました。このメンブレンを歯周治療に応用できるかラット歯槽骨欠損モデルを用いて再生効果を検討します。

Outline of Final Research Achievements

Periodontitis is a chronic disease caused by inflammatory bone resorption resulting in tooth mobility and loss. By adding enamel matrix protein to the membrane of a periodontal tissue regenerative therapy, the applicants created a new membran which formed calcification on the membrane surface in a few hours in simulated body fluid buffer abd had more than twice the adhesive strength to dentin than a conventional membrane.
To investigate the effect on alveolar bone regeneration in vivo, experiments were performed in pigs. Although no significant differences were observed in this experiment due to the experimental design, the placement of the membrane in bone defects resulted in greater bone regeneration, suppression of gingival retraction, and suppression of long epithelial attachments than did the regular membranes over a period of up to 12 weeks.

Academic Significance and Societal Importance of the Research Achievements

歯周炎は、炎症性の骨吸収により、歯の動揺、喪失が引き起こされる慢性疾患であり、これまで、様々な歯周組織再生材料が開発され、使用されてきた。今回作成した申請者らのメンブレンは、メンブレン自体が石灰化し、象牙質にメンブレンが接着する性質を持ち、通常のメンブレンよりも骨の再生の促進や、歯肉退縮の抑制、長い上皮付着の抑制などの効果を得られる可能性が示された。このことは、このメンブレンが将来、歯周組織再生治療へ応用できる可能性を示している。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (3 results)

All 2022 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results)

  • [Int'l Joint Research] トロント大学/ウエスタン大学(カナダ)

    • Related Report
      2022 Research-status Report
  • [Journal Article] Amelotin Promotes Mineralization and Adhesion in Collagen-Based Systems2022

    • Author(s)
      Ikeda Yuichi、Holcroft James、Ikeda Eri、Ganss Bernhard
    • Journal Title

      Cellular and Molecular Bioengineering

      Volume: 15 Issue: 3 Pages: 245-254

    • DOI

      10.1007/s12195-022-00722-2

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Screening of functionalized collagen membranes with a porcine periodontal regeneration model2022

    • Author(s)
      Quan Bryan D.、Sadeghi Rokhsareh、Ikeda Yuichi、Ganss Bernhard、Hamilton Douglas W.、McCulloch Christopher A.、Sone Eli D.
    • Journal Title

      Oral Diseases

      Volume: - Issue: 7 Pages: 2845-2853

    • DOI

      10.1111/odi.14445

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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