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A Mechanobiological Approach to Elucidate the Control Mechanism of Zirconia Biocompatibility

Research Project

Project/Area Number 21K17003
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 57040:Regenerative dentistry and dental engineering-related
Research InstitutionTohoku University

Principal Investigator

SUN LU  東北大学, 歯学研究科, 助教 (10897606)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsosteoblast / zirconia / cell adhesion / syndecan / RGD / osteoblastic cell / HSPGs / ジルコニア / メカノバイオロジー / 生体親和性
Outline of Research at the Start

本研究では、RNAiスクリーニングを用いてナノジルコニアへの骨芽細胞の接着と形態維持に必須なHSPG遺伝子Xを同定する。次に、この遺伝子Xの存在が、メカノバイオロジカルな骨芽細胞分化の制御因子であるERKの活性に与える影響を検討する。また、遺伝子Xの変異体導入による細胞形態やジルコニアに対する接着動態の検討により、遺伝子Xのメカノバイオロジカルな細胞形態制御機構を解明し。マイクロアレイにより、関連遺伝子間のクロストークについて検討を行い、骨芽細 胞の活性化機構を明らかにする。以上により、ジルコニア材料の生体親和性がHSPG遺伝子Xを介して発揮される分子メカニズムを明らかにすることを目的とする。

Outline of Final Research Achievements

We introduced two osteoblastic cell lines-- MC3T3-E1 and Saos-2 cells into this research. We seed the cells respectively on the control plate, CpTi, 3Y-TZP and NanoZr for 14 days. Then the cell proliferation ability and ALP ability was detected The result shows that both cell lines can attach and culture well on all materials and Saos2 cells show a better osteogenesis ability on zirconia and titanium than the control group. However, MC3T3-E1 cells could not conduct the osteogenesis ability well on all materials. Interestingly, in PCR tests of Saos-2 cells group, syndecan shows an increased expression on CpTi but a low expression on 3Y-TZP and NanoZr. The different expression levels of syndecans indicate that HSPGs might induce different mechanisms on titanium and zirconia. We pre-treate the cells with RDG (Arg-Fly-Asp-Ser) and nagstive control reagent RGE (Arg-Fly-Glu-Ser) and intend to detect the expression of syndecan family on different materials.

Academic Significance and Societal Importance of the Research Achievements

Zirconia is a potential material for dental implants, but still exist shortcuts such as high toughness, low-temperature degradation and long-term stability. In this study, we intend to investigate the cell ability on zirconia, detect the mechanism behind, and aiming to improve zirconia materials.

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (5 results)

All 2023 2022 2021

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] Questionnaire survey on the satisfaction of SimEx dental education system2023

    • Author(s)
      Hong Guang、Hung Chun-Cheng、Mayanagi Gen、Nishioka Takashi、Sun Lu、Lai Eddie Hsiang-Hua、Lan Ting-Hsun、Sasaki Keiichi、Takahashi Nobuhiro
    • Journal Title

      Journal of Dental Sciences

      Volume: 18 Issue: 2 Pages: 840-847

    • DOI

      10.1016/j.jds.2023.01.028

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Surface Modifications for Zirconia Dental Implants: A Review2021

    • Author(s)
      Sun L, Hong G.
    • Journal Title

      Front. Dent. Med.

      Volume: 2 Pages: 733242-733242

    • DOI

      10.3389/fdmed.2021.733242

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] The role of syndecan on osteoblastic cell adhesion of nano-zirconia2022

    • Author(s)
      SUN Lu
    • Organizer
      The International Oral Health Symposium 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The viability of ceramic dental implants2022

    • Author(s)
      SUN Lu
    • Organizer
      2022 CA+inD Summer Short-term Exchange Program Multimodal Global Leaders Development through Asian-Model Dentistry Consortium
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Introduction of International Collaborative and Innovative Dentistry2022

    • Author(s)
      SUN Lu
    • Organizer
      TU- FJMU International Workshop
    • Related Report
      2022 Annual Research Report
    • Invited

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Published: 2021-04-28   Modified: 2024-01-30  

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