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A new method to analyze for ecoogical structure by measuring stable isotope ratios of microbial nucleic acids

Research Project

Project/Area Number 21K17879
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 63010:Environmental dynamic analysis-related
Research InstitutionResearch Institute for Humanity and Nature (2022-2023)
Kyoto University (2021)

Principal Investigator

Onishi Yuji  総合地球環境学研究所, 基盤研究部, 特任助教 (10847677)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords安定同位体 / 核酸 / 琵琶湖 / 底生動物 / 硫黄 / リン / 微生物
Outline of Research at the Start

炭素・窒素安定同位体比測定に適した微生物からの簡便な核酸抽出手法を検討する。その後、様々な微生物を炭素・窒素・リン濃度を様々に変化させた培地で培養し、菌体と培地中の炭素窒素源との同位体比の差を測定する。さらに、核酸・アミノ酸・脂肪酸などの代謝物質の化合物ごとの濃度プロファイルと炭素・窒素同位体比を測定することで、核酸の生合成代謝の活性と菌体と栄養源との同位体比の差との関連を考察する。また、これらの結果を琵琶湖深層の生態系に適用することで、新たな生態系解析手法の確立を目指す。

Outline of Final Research Achievements

We investigated methods for recovery, purification, and isotope ratio measurements of microbial nucleic acids to establish a new method for accurately assessing the importance of microbial activity for nutrient resources supporting benthic animal communities in freshwater ecosystems. As a result, it became clear that the purification was problematic and that isotope ratios could not be successfully measured by the conventional methods. However, we established a new evaluation method for microbial activity using sulfur isotope ratios in freshwater ecosystems, and found that sulfur cycling in sediments and associated microbial activities are important as a nutrient resource for benthic animal communities.

Academic Significance and Societal Importance of the Research Achievements

淡水湖沼生態系において、堆積物中での硫黄酸化細菌や硫酸還元細菌の活動が、底生動物群集の栄養源として重要であることが初めて明らかとなった。これは、淡水湖沼生態系内にこれまで知られていない新たな栄養経路が存在していることを示唆している。この現象について、他の湖沼での調査を進めることによって、地球温暖化などの環境変動に対する淡水湖沼生態系の応答を理解する上で重要な知見が得られると期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (11 results)

All 2024 2023 2022 2021

All Journal Article (6 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] 淡水湖沼における底生動物群集を支える堆積物中の微生物活動2024

    • Author(s)
      大西雄二
    • Journal Title

      アグリバイオ

      Volume: 8 Pages: 42-44

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Major contribution of sulfide‐derived sulfur to the benthic food web in a large freshwater lake2023

    • Author(s)
      Onishi Yuji、Yamanaka Toshiro、Koba Keisuke
    • Journal Title

      Geobiology

      Volume: 21 Issue: 5 Pages: 671-685

    • DOI

      10.1111/gbi.12569

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multipath ecological influence of an iteroparous fish migration from Lake Biwa to an alluvial stream2023

    • Author(s)
      Akira Kurasawa, Yuji Onishi, Keisuke Koba, Keitaro Fukushima, Hiromi Uno
    • Journal Title

      Freshwater Biology

      Volume: 68 Issue: 8 Pages: 1400-1412

    • DOI

      10.1111/fwb.14112

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multipath ecological influences of an iteroparous fish migration from Lake Biwa to a moderate sized alluvial river2023

    • Author(s)
      Kurasawa A., Onishi Y., Fukushima K., Koba K., Uno H.
    • Journal Title

      Freshwater Biology

      Volume: -

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] 琵琶湖の底生動物の炭素・窒素・硫黄安定同位体比2022

    • Author(s)
      大西雄二
    • Journal Title

      金属

      Volume: 92 Pages: 92-98

    • Related Report
      2022 Research-status Report
  • [Journal Article] Digestion Efficiency during Alkaline Persulfate Oxidation for Determination of Total Phosphorus Content of Biological Samples2021

    • Author(s)
      ONISHI Yuji
    • Journal Title

      Analytical Sciences

      Volume: 37 Issue: 12 Pages: 1771-1774

    • DOI

      10.2116/analsci.21P116

    • NAID

      130008127635

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2021-12-10
    • Related Report
      2021 Research-status Report
  • [Presentation] 成層期の琵琶湖における堆積物中硫黄循環と底生動物への影響2023

    • Author(s)
      大西雄二,山中寿朗,木庭啓介
    • Organizer
      Japan Geoscience Union Meeting 2023
    • Related Report
      2023 Annual Research Report
  • [Presentation] 循環期の琵琶湖における硫化物と底生動物の硫黄同位体比2023

    • Author(s)
      大西雄二,木庭啓介
    • Organizer
      第13回同位体環境学シンポジウム
    • Related Report
      2023 Annual Research Report
  • [Presentation] 遡上魚が排泄するアンモニウムと河川生物の窒素同位体比2023

    • Author(s)
      大西雄二、倉澤央、宇野裕美、福島慶太郎、木庭啓介
    • Organizer
      生態学会第70回年会
    • Related Report
      2022 Research-status Report
  • [Presentation] Biogeochemical sulfur cycle and contribution of the sulfide to benthic animals in freshwater lake2022

    • Author(s)
      Onishi Y/. Yamanaka T., Koba K.
    • Organizer
      12th International Conference on the Applications of Stable Isotope Techniques to Ecological Studies (IsoEcol 2022)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 成層期における琵琶湖堆積物中硫化物の濃度と同位体比の鉛直分布2022

    • Author(s)
      大西雄二、山中寿朗、木庭啓介
    • Organizer
      Japan Geoscience Union Meeting 2022
    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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