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Can obligate hydrogen-utilizing organohalide respiring bacteria use electrons from electrodes as an energy source?

Research Project

Project/Area Number 21K17900
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 64010:Environmental load and risk assessment-related
Research InstitutionNagoya Institute of Technology

Principal Investigator

MENG LINGYU  名古屋工業大学, 工学(系)研究科(研究院), 特任助教 (10859440)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsバイオレメディエーション / 電気化学脱塩素化 / 電子伝達 / 単離 / 低環境負荷 / Dehalococcoides / Desulfosporosinus / 電気化学的な脱塩素化 / 水素伝達 / 電気化学脱塩素化反応 / 細胞外電子伝達 / 電子伝達機構
Outline of Research at the Start

有機ハロゲンは世界的な地下水汚染物質であり,汚染を解消するべく微生物を用いたバイオレメディエーションが積極的に取られるようになってきた.しかし浄化過程において, 浄化を担う微生物よりも現場で生育する雑多な微生物が必要以上に増殖することが観察されており,浄化を担う脱ハロゲン化呼吸細菌(ORB)へ低リスクに電子供給できる手法が求められる. 本研究は従来の乳酸などC3以上の高分子炭化水素を発酵してORBに水素を供給するのではなく,電極からORBへの電子供給する方法の確立に取り組む.

Outline of Final Research Achievements

The primary objective of this study was to ascertain the functional microorganisms accountable for electron transfer between the electrode and strain NIT01. Furthermore, the study aimed to elucidate the electron-transfer mechanism employed by Dehalococcoides mccartyi NIT01, a selective hydrogenotrophic dehalogenating respiratory bacterium, in utilizing electrons from the electrode for dechlorination. In order to accomplish these objectives, functional microorganisms responsible for electron transfer between the electrode and strain NIT01 were identified and isolated. Notably, a novel species called Desulfosporosinus sp. strain MM01 was discovered, exhibiting a 95.2% and 96.2% homology to the known genera Meridiei and Orientis, respectively. These findings also demonstrated that Desulfosporosinus played a vital role in promoting electrochemical hydrogen production and contributed significantly to the dechlorination process of strain NIT01.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、偏性水素資化性脱ハロゲン化呼吸細菌であるDehalococcoides mccartyi NIT01株が電極活性菌を介した電子伝達機構を解明した点にある。Desulfosporosinus sp. MM01株という新種の微生物が電子伝達を担う機能微生物として特定され、その電気化学的な水素生成能力と脱塩素化への寄与も明らかにした。これにより、塩素化合物を無害化する微生物の機能についての知見拡充ができた。社会的意義としては、NIT01株とMM01株を利用する電気化学脱塩素化が低環境負荷なバイオレメディエーション技術として期待できる。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results) Book (1 results) Funded Workshop (2 results)

  • [Int'l Joint Research] Harbin Institute of Technology(中国)

    • Related Report
      2022 Annual Research Report
  • [Journal Article] 6月 Soil microorganisms facilitated the electrode-driven trichloroethene dechlorination to ethene by Dehalococcoides species in a bioelectrochemical system2022

    • Author(s)
      Lingyu Meng , Naoko Yoshida , Zhiling Li
    • Journal Title

      Environmental Research

      Volume: 209 Pages: 112801-112801

    • DOI

      10.1016/j.envres.2022.112801

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reduced graphene oxide increases cells with enlarged outer membrane of Citrifermentans bremense and exopolysaccharides secretion2022

    • Author(s)
      Lingyu Meng, Li Xie, Yuu Hirose, Takumi Nishiuchi, Naoko Yoshida
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 218 Pages: 114754-114754

    • DOI

      10.1016/j.bios.2022.114754

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Electro-active soil microorganisms mediated the electron transfer for the dechlorination of trichloroethene by pure Dehalococcoides culture2021

    • Author(s)
      Lingyu Meng, Naoko Yoshida
    • Organizer
      5th Asia-Pacific International Society of Microbial Electrochemistry and Technology Conference (5th AP-ISMET)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Exploring the microorganisms for achieving the bioelectrochemical dechlorination of Dehalococcoides via extracellular electron transfer2021

    • Author(s)
      Lingyu Meng, Naoko Yoshida
    • Organizer
      DehaloconIII
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Co-metabolism of Desulfosporosinus with Dehalococcoides achieved full dechlorination in a bioelectrochemical system2021

    • Author(s)
      Lingyu Meng, Naoko Yoshida
    • Organizer
      JSME2021
    • Related Report
      2021 Research-status Report
  • [Book] 生物電気化学システムにおける微生物と電極間の相互作用2021

    • Author(s)
      孟令宇、吉田奈央子
    • Total Pages
      6
    • Publisher
      技術情報協会
    • ISBN
      9784861048678
    • Related Report
      2021 Research-status Report
  • [Funded Workshop] ISMET8 - International Society for Microbial Electrochemistry and Technology2022

    • Related Report
      2022 Annual Research Report
  • [Funded Workshop] The Water and Environment Technology Conference online 2022 (JWET)2022

    • Related Report
      2022 Annual Research Report

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Published: 2021-04-28   Modified: 2024-01-30  

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