3D analysis of chromatin structure in cell nucleus with electron microscopy and mathematical modeling

• Project/Area Number: 21K18234
• Research Category: Grant-in-Aid for Challenging Research (Pioneering)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 43:Biology at molecular to cellular levels, and related fields
• Research Institution: Hiroshima University
• Principal Investigator: 楯 真一 広島大学, 統合生命科学研究科(理), 教授 (20216998)
• Co-Investigator(Kenkyū-buntansha): 粟津 暁紀 広島大学, 統合生命科学研究科(理), 准教授 (00448234)
• Project Period (FY): 2021-07-09 – 2026-03-31
• Project Status: Granted (Fiscal Year 2024)
• Budget Amount: ¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000); Fiscal Year 2024: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2023: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000); Fiscal Year 2022: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2021: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 核内クロマチン構造 / 電子顕微鏡 / 数理モデル / 分裂酵母

Outline of Research at the Start

FIB-SEMを用いた核内クロマチン構造の3次元電子顕微鏡像観測，基準点となるスピンドル極体(SPB)と標的とする遺伝子座に蛍光タンパク質で標識導入した分裂酵母を対象に，核内での遺伝子座の動態計測を共焦点顕微鏡で行う．2つの計測データをもとに，粗視化モデルとして核内クロマチン構造・動態を表現する数理モデルを構築する．構築する数理モデルを用いて，核内クロマチン構造・動態をシミュレートすることにより，核内クロマチン高次構造の遺伝情報制御における役割を解明する．

Outline of Annual Research Achievements

2024年度は、クロマチン構造動態モデルを最適化してモデルとして完成とともに，そのモデルから取り出せる構造・動態情報の検証を集中的に進めた．
理研・新海博士の協力を得て，彼が開発したPHi-Cを元にして131カ所の遺伝子座の動態データと，Hi-Cから得られている遺伝子座間の近接データを元にして，遺伝子座間に仮想的なポテンシャルを構築することで，分裂酵母の内の3つの染色体の立体構造・動態シミュレーションを可能とした．
今期は，各クロマチン構造・動態シミュレーションを詳細に解析することで，その立体構造・動態の生物学的な意味について解析を進めた．シミュレーションデータから，各染色体のセントロメア，テロメア間の距離の分布を解析したところ，実験データと高い整合性が確認された．モデルの妥当性が確認できた．
分裂酵母のセントロメアはSPBと呼ばれる一カ所に集積しているが，SPBは周期的な揺動運動をしており，その結果クロマチン構造全体が大きく揺動することが予想されていたが，今回のPHi-Cモデルによる解析では，セントロメア周辺は大きく揺動するも，セントロメアから離れるにつれて，クロマチン構造の運動は急激に低下するという特徴が明らかになった．クロマチン構造は核内では意外なほど同じ形態を維持しているという意外性が見いだされた．
このことは，クロマチン構造の電子顕微鏡像再構築の際には，むしろ有利に働く．今期の成果をもとに，FIB-SEM像の再構築へと研究を進める．

Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

クロマチン構造・動態モデルは当初予定してよりも格段に精度の高い完成度の高いものにすることができた．一方で，2024年度中は，研究代表者の家庭で家人が逝去、別の家族が入院治療するなどのため、代表者の研究時間が分断されたため，モデル構造からすぐに電子顕微鏡像解析という最終ゴールに到達する時間が十分にとれない事情があった．このため，一年間研究期間を延長して，最終的なゴールまで研究を進める．
2024年度の解析により，当初予想していたのとは異なり，分裂酵母の核内クロマチン構造は意外なほど大きな構造変化をしておらず，むしろ一定の立体構造を保つという描像が得られたのは電子顕微鏡像解析を行う上では，構造多形を仮定した解析が必要なくなったために，計画よりもスムーズに進めることができることがわかった．

Strategy for Future Research Activity

2024年度は，研究代表者の私的な事情もあり，クロマチン構造・動態モデルに基づいた電子顕微鏡像解析という最終ゴールへの到達ができなかったが，一年の延長期間のうちに確実に電子顕微鏡像解析まで進める．
これまでの段階で，想定した以上に高精度なクロマチン構造・動態モデルの構築ができたことは強調したい．膨大な独自の実験データに基づいて，オリジナルな統計物理モデルによる独自のクロマチン構造モデルである．現在，本研究で開発したクロマチン構造・動態モデルの部分の論文を作成しており，2025年中には論文発表する予定である．

Research Products

• [Int'l Joint Research] ＮＩＨ(米国)
• [Int'l Joint Research] University of Padova(イタリア)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] ＮＩＨ(米国)
• [Int'l Joint Research] Seoul National University/UNIST Central Research Facilities(韓国)
• [Int'l Joint Research] University of Padova(イタリア)
• [Int'l Joint Research] National Institute of Health (NIH)(米国)
• [Int'l Joint Research] Seoul National University/UNIST Central Research Facilities(韓国)
• [Int'l Joint Research] University of Padova(イタリア)
• [Int'l Joint Research] National Institute of Health (NIH)(米国)
• [Journal Article] Backbone resonance assignments of dopamine N-acetyltransferase in free and cofactor-bound states (2025)
  • Author(s): Wu Chu-Ya、Lee Yi-Zong、Hu I-Chen、Chiu Liang-Yuan、Ding Wei-Cheng、Wang Jing、Sue Shih-Che、Tate Shin-Ichi、Lyu Ping-Chiang
  • Journal Title: Biomolecular NMR Assignments Volume: - Issue: 1 Pages: 83-93
  • DOI: 10.1007/s12104-025-10222-9
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Impacts of D-aspartate on the Aggregation Kinetics and Structural Polymorphism of Amyloid β Peptide 1-42 (2025)
  • Author(s): Hsiao Li-Ching、Lee Chih-Hsuan、Mazmanian Karine、Yoshida Masaya、Ito Genta、Murata Takuya、Utsunomiya-Tate Naoko、Haino Takeharu、Tate Shih-ichi、Hsu Shang-Te Danny
  • Journal Title: Journal of Molecular Biology Volume: 437 Issue: 12 Pages: 169092-169092
  • DOI: 10.1016/j.jmb.2025.169092
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Potential multiple disease progression pathways in female patients with Alzheimer’s disease inferred from transcriptome and epigenome data of the dorsolateral prefrontal cortex (2025)
  • Author(s): Honda Kousei、Awazu Akinori
  • Journal Title: PLOS ONE Volume: 20 Issue: 3 Pages: e0313733-e0313733
  • DOI: 10.1371/journal.pone.0313733
  • Peer Reviewed / Open Access
• [Journal Article] Conformation Regulation of Trisresorcinarene Directed by Cavity Solvation (2024)
  • Author(s): Shimoyama Daisuke、Sekiya Ryo、Inoue Shoichiro、Hisano Naoyuki、Tate Shin‐ichi、Haino Takeharu
  • Journal Title: Chemistry A European Journal Volume: 30 Issue: 63
  • DOI: 10.1002/chem.202402922
  • Peer Reviewed / Open Access
• [Journal Article] Effects of novel lactoferrin peptides on LPS‐induced alveolar bone destruction in a rat model (2024)
  • Author(s): Yamada Sakura、Chea Chanbora、Furusho Hisako、Oda Kanae、Shiba Fumie、Tanimoto Kotaro、Tate Shin‐ichi、Miyauchi Mutsumi、Takata Takashi
  • Journal Title: Chemical Biology & Drug Design Volume: 104 Issue: 1
  • DOI: 10.1111/cbdd.14574
  • Peer Reviewed
• [Journal Article] Controlled Helical Organization in Supramolecular Polymers of Pseudo-Macrocyclic Tetrakisporphyrins (2024)
  • Author(s): Fujii Naoka、Hisano Naoyuki、Hirao Takehiro、Kihara Shin‐ichi、Tanabe Kouta、Yoshida Masaya、Tate Shin‐ichi、Haino Takeharu
  • Journal Title: Angewandte Chemie International Edition Volume: 64 Issue: 5
  • DOI: 10.1002/anie.202416770
  • Peer Reviewed / Open Access
• [Journal Article] Quantitative prediction of rice starch digestibility using Raman spectroscopy and multivariate calibration analysis (2024)
  • Author(s): Ichinose Junya、Oba Kenji、Arase Yuya、Kaneshiro Junichi、Tate Shin-ichi、Watanabe Tomonobu M
  • Journal Title: Food Chemistry Volume: 435 Pages: 137505-137505
  • DOI: 10.1016/j.foodchem.2023.137505
  • Peer Reviewed
• [Journal Article] Assembly of continuous high‐resolution draft genome sequence of Hemicentrotus pulcherrimus using long‐read sequencing (2024)
  • Author(s): Komoto Tetsushi、Ikeo Kazuho、Yaguchi Shunsuke、Yamamoto Takashi、Sakamoto Naoaki、Awazu Akinori
  • Journal Title: Development, Growth & Differentiation Volume: 66 Issue: 4 Pages: 297-304
  • DOI: 10.1111/dgd.12924
  • Peer Reviewed / Open Access
• [Journal Article] Possibilities of skin coat color‐dependent risks and risk factors of squamous cell carcinoma and deafness of domestic cats inferred via RNA‐seq data (2023)
  • Author(s): Awazu Akinori、Takemoto Daigo、Watanabe Kaichi、Sakamoto Naoaki
  • Journal Title: Genes to Cells Volume: 28 Issue: 12 Pages: 893-905
  • DOI: 10.1111/gtc.13076
  • Peer Reviewed / Open Access
• [Journal Article] The crucial role of CTCF in mitotic progression during early development of sea urchin (2023)
  • Author(s): Watanabe Kaichi、Fujita Megumi、Okamoto Kazuko、Yoshioka Hajime、Moriwaki Miki、Tagashira Hideki、Awazu Akinori、Yamamoto Takashi、Sakamoto Naoaki
  • Journal Title: Development, Growth and Differentiation Volume: 65 Issue: 7 Pages: 395-407
  • DOI: 10.1111/dgd.12875
  • Peer Reviewed
• [Journal Article] Mathematical model of structural changes in nuclear speckle (2023)
  • Author(s): Wakao, S., Saitoh, N., Awazu, A.
  • Journal Title: Biophysics and Physicobiology Volume: 20 Issue: 2 Pages: n/a
  • DOI: 10.2142/biophysico.bppb-v20.0020
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Formation of Small-World Network Containing Module Networks in Globally and Locally Coupled Map System with Changes in Global Connection with Time Delay Effects (2023)
  • Author(s): Nakanishi Taito、Awazu Akinori
  • Journal Title: Journal of the Physical Society of Japan Volume: 92 Issue: 3 Pages: 034801-034801
  • DOI: 10.7566/jpsj.92.034801
  • Peer Reviewed / Open Access
• [Journal Article] Identification of aberrant transcription termination at specific gene loci with DNA hypomethylated transcription termination sites caused by DNA methyltransferase deficiency (2022)
  • Author(s): Masaki Shirai, Takuya Nara, Haruko Takahashi, Kazuya Takayama, Yuan Chen, Yudai Hirose, Masashi Fujii, Akinori Awazu, Nobuyoshi Shimoda, and Yutaka Kikuchi
  • Journal Title: Genes & Genetic Systems Volume: 97 Issue: 3 Pages: 139-152
  • DOI: 10.1266/ggs.21-00092
  • ISSN: 1341-7568, 1880-5779
  • Year and Date: 2022-06-01
  • Peer Reviewed / Open Access
• [Journal Article] Valosin‐containing protein (VCP) regulates the stability of fused in sarcoma (FUS) granules in cells by changing ATP concentrations (2022)
  • Author(s): Yasuda Kyota、Watanabe Tomonobu M.、Kang Myeong‐Gyun、Seo Jeong Kon、Rhee Hyun‐Woo、Tate Shin‐ichi
  • Journal Title: FEBS Letters Volume: - Issue: 11 Pages: 1412-1423
  • DOI: 10.1002/1873-3468.14353
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Identification of embryonic RNA granules that act as sites of mRNA translation after changing their physical properties (2022)
  • Author(s): Sato Keisuke、Sakai Moeko、Ishii Anna、Maehata Kaori、Takada Yuki、Yasuda Kyota、Kotani Tomoya
  • Journal Title: iScience Volume: 25 Issue: 6 Pages: 104344-104344
  • DOI: 10.1016/j.isci.2022.104344
  • Peer Reviewed / Open Access
• [Journal Article] FUS液液相分離の機能と細胞内相分離制御機構 (2022)
  • Author(s): 安田恭大，楯 真一
  • Journal Title: 細胞 Volume: 54 Pages: 448-451
• [Journal Article] Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation of mouse embryonic stem cells (2022)
  • Author(s): Komoto Tetsushi、Fujii Masashi、Awazu Akinori
  • Journal Title: Biophysics and Physicobiology Volume: 19 Issue: 0 Pages: n/a
  • DOI: 10.2142/biophysico.bppb-v19.0018
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Molecular dynamics analysis of biomolecular systems including nucleic acids (2022)
  • Author(s): Kameda Takeru、Awazu Akinori、Togashi Yuichi
  • Journal Title: Biophysics and Physicobiology Volume: 19 Issue: 0 Pages: n/a
  • DOI: 10.2142/biophysico.bppb-v19.0027
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Mathematical model of chromosomal dynamics during DNA double strand break repair in budding yeast (2022)
  • Author(s): Nakahata Shinjiro、Komoto Tetsushi、Fujii Masashi、Awazu Akinori
  • Journal Title: Biophysics and Physicobiology Volume: 19 Issue: 0 Pages: n/a
  • DOI: 10.2142/biophysico.bppb-v19.0012
  • ISSN: 2189-4779
  • Peer Reviewed / Open Access
• [Journal Article] Partial exogastrulation due to apical-basal polarity of F‐actin distribution disruption in sea urchin embryo by omeprazole (2022)
  • Author(s): Watanabe Kaichi、Yasui Yuhei、Kurose Yuta、Fujii Masashi、Yamamoto Takashi、Sakamoto Naoaki、Awazu Akinori
  • Journal Title: Genes to Cells Volume: - Issue: 6 Pages: 1-17
  • DOI: 10.1111/gtc.12934
  • Peer Reviewed / Open Access
• [Journal Article] Ribosomal protein L5 facilitates rDNA-bundled condensate and nucleolar assembly (2022)
  • Author(s): Matsumori Haruka、Watanabe Kenji、Tachiwana Hiroaki、Fujita Tomoko、Ito Yuma、Tokunaga Makio、Sakata-Sogawa Kumiko、Osakada Hiroko、Haraguchi Tokuko、Awazu Akinori、Ochiai Hiroshi、Sakata Yuka、Ochiai Koji、Toki Tsutomu、Ito Etsuro、Goldberg Ilya G、Tokunaga Kazuaki、Nakao Mitsuyoshi、Saitoh Noriko
  • Journal Title: Life Science Alliance Volume: 5 Issue: 7 Pages: 1-18
  • DOI: 10.26508/lsa.202101045
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Self-Organization of Diverse Directional Hierarchical Networks in Simple Coupled Maps with Connection Changes (2022)
  • Author(s): Nakanishi Taito、Fujii Masashi、Awazu Akinori
  • Journal Title: Journal of the Physical Society of Japan Volume: 91 Issue: 2 Pages: 023801-023801
  • DOI: 10.7566/jpsj.91.023801
  • NAID: 40022824583
  • Peer Reviewed / Open Access
• [Journal Article] Path ensembles for Pin1-catalyzed cis-trans isomerization of a substrate calculated by weighted ensemble simulations (2021)
  • Author(s): Kei Moritsugu, Norifumi Yamamoto, Yasushige Yonezawa, Shin-ichi Tate, and Hiroshi Fujisaki
  • Journal Title: Journal of Chemical Theory and Computation Volume: 17 Issue: 4 Pages: 2522-2529
  • DOI: 10.1021/acs.jctc.0c01280
  • Peer Reviewed
• [Journal Article] Hepta-Histidine Inhibits Tau Aggregation (2021)
  • Author(s): Kondo Kanoh、Ikura Teikichi、Tanaka Hikari、Fujita Kyota、Takayama Sumire、Yoshioka Yuki、Tagawa Kazuhiko、Homma Hidenori、Liu Su、Kawasaki Ryosuke、Huang Yong、Ito Nobutoshi、Tate Shin-ichi、Okazawa Hitoshi
  • Journal Title: ACS Chemical Neuroscience Volume: 12 Issue: 16 Pages: 3015-3027
  • DOI: 10.1021/acschemneuro.1c00164
  • Peer Reviewed / Open Access
• [Journal Article] Ultrasensitive Nucleosome Binding Regulation Mediated by Intrinsically Disordered Regions (2021)
  • Author(s): TATE Shin-ichi
  • Journal Title: Seibutsu Butsuri Volume: 61 Issue: 5 Pages: 312-315
  • DOI: 10.2142/biophys.61.312
  • NAID: 130008093155
  • ISSN: 0582-4052, 1347-4219
  • Peer Reviewed / Open Access
• [Presentation] 天然変性タンパク質の構造ダイナミクス研究のための新たな計測と解析技術 (2025)
  • Author(s): 楯 真一
  • Organizer: 第7回広帯域極限電磁波生命理工連携研究会
  • Invited
• [Presentation] hnRNPs bi-directionally regulate stress granule growth and disassembly (2024)
  • Author(s): Kyota Yasuda, Miyu Murata, Yuto Saito, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: 第47回日本分子生物学会年会
• [Presentation] Alignment reagents for NMR studies with customizability of salt types/concentration to enable facile residual dipolar couplings (RDCs) measurements (2024)
  • Author(s): 清水駿、武川純、古板恭子、内田紀之、石田康、児嶋長次郎、楯真一、佐々木高義、浜口仁
  • Organizer: 第63回 NMR討論会
• [Presentation] 公共データの解析によるイエネコの被毛の色依存的な難聴と扁平上皮癌リスクの新規機序推定 (2024)
  • Author(s): 粟津 暁紀、竹本 大悟、渡辺 開智、後藤 梨久都、坂本 尚昭
  • Organizer: 第167回日本獣医学会学術集会
• [Presentation] Spatio-temporal control of biomolecular condensation and the invovlment of amino acid chrality in it (2024)
  • Author(s): Kyota Yasuda, Rina Mihara, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: WPI-SKCM2 Spring Symposium
  • Invited
• [Presentation] Time-resolved proteomics of stress granules to identify key regulatory proteins to be expplored by NMR (2024)
  • Author(s): Miyu Murata, Kyota Yasuda, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: Taiwan-Japan NMR symposium
  • Int'l Joint Research
• [Presentation] Regulatory plays of intrinsically disordered regions (IDRs) in proteins revealed by NMR with multimodal approaches (2023)
  • Author(s): Shin-ichi Tate
  • Organizer: The 10th Asia-Pacific NMR Symposium 2023
  • Int'l Joint Research / Invited
• [Presentation] Spatio-temporal control of biomolecular condensation and its association to the human disease (2023)
  • Author(s): Kyota Yasuda, Rina Mihara, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: Mathematical and life science program symposium
  • Invited
• [Presentation] Structure biology on unstructured proteins - how the intrinsically disordered proteins (IDPs) work (2023)
  • Author(s): Shin-ichi Tate
  • Organizer: Mathematical and life science program symposium
  • Invited
• [Presentation] Protein condensates and their relevance to neurodegenerative disease as knotty social problems (2023)
  • Author(s): Shin-ichi Tate
  • Organizer: WPI-SKCM2 Poland Symposium
  • Int'l Joint Research / Invited
• [Presentation] Spatio-Temporal Control of Biomolecular Condensation and the Role of Amino Acid Chirality (2023)
  • Author(s): Kyota Yasuda, Rina Mihara, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: WPI-SKCM2 Winter School Lecture
  • Invited
• [Presentation] Identify and functional analysis of factors for stress granule-disassembly via Interactome analysis (2023)
  • Author(s): Miyu Murata, Kyota Yasuda, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: The 46th Annual Meeting of the Molecular Biology Society of Japan
• [Presentation] Proximity labeling identified a protein essential for the proper formation and function of stress granule (2023)
  • Author(s): Kyota Yasuda, Rina Mihara, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: The 46th Annual Meeting of the Molecular Biology Society of Japan
• [Presentation] Proximity labeling identified a protein essential for the proper formation and function of stress granule (2023)
  • Author(s): Kyota Yasuda, Rina Mihara, Hyun-woo Rhee, Shin-ichi Tate
  • Organizer: The 61st Annual Meeting of the Biophysical Society of Japan
• [Presentation] Exploring the mechanism to explain the difference in protein liquid-liquid phase separation (LLPS) processes between in vitro and in cells (2023)
  • Author(s): Hitomi Kimura, Kyota Yasuda, Natsumi Tane, Shin-ichi Tate
  • Organizer: The 61st Annual Meeting of the Biophysical Society of Japan
• [Presentation] Structure dynamics of intrinsically disordered proteind (IDPs) and their biological roles (2022)
  • Author(s): Shin-ichi Tate
  • Organizer: The 7th International Symposium on Drug Discovery and Design by NMR
  • Int'l Joint Research / Invited
• [Presentation] NMRによる天然変性タンパク質の構造ダイナミクスと昨日との相関解析 (2022)
  • Author(s): 楯 真一
  • Organizer: 広島大学統合生命科学研究科シンポジウム「分子原子レベルの統合生命科学研究」
  • Invited
• [Presentation] Exogastrulation due to cytoskeletal polarity distribution in sea urchin embryo (2022)
  • Author(s): Kaichi Watanabe, Yuhei Yasui, Yuta Kurose, Naoaki Sakamoto, Akinori Awazu
  • Organizer: The 60th Annual Meeting of the Biophysical Society of Japan
• [Presentation] Epigenetic-structural changes in X chromosomes promote Xic pairing during early differentiation process from embryonic stem cell of mouse (2022)
  • Author(s): Tetsushi Komoto, Masashi Fujii, Akinori Awazu
  • Organizer: The 60th Annual Meeting of the Biophysical Society of Japan
• [Presentation] Simulations of structural dynamics of nuclear speckle (2022)
  • Author(s): Shingo Wakao, Masashi Fuji, Akinori Awazu
  • Organizer: The 60th Annual Meeting of the Biophysical Society of Japan
• [Presentation] Chirality Disruption in Protein Dynamics (2021)
  • Author(s): Shin-ichi Tate
  • Organizer: Molecular Chirality 2021
  • Invited
• [Presentation] ウニにおける H+/K+イオンポンプ活性は胚の細胞骨格分布極性を高めて原腸形成を促進させる (2021)
  • Author(s): 渡辺 開智, 安井 優平, 黒瀬 友太, 坂本 尚昭, 粟津 暁紀
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] 核スペックルの構造形成・動態のシミュレーション (2021)
  • Author(s): 若尾 真吾, 藤井 雅史, 粟津 暁紀
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] 空間的局所相互作用を伴う動的・可塑的ネットワーク系の自発的構造形成 (2021)
  • Author(s): 中西 大斗, 藤井 雅史, 粟津 暁紀
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] 出芽酵母の DNA 二本鎖切断時における染色体動態の数理モデル (2021)
  • Author(s): 中畑 伸児郎, 藤井 雅史, 粟津 暁紀
  • Organizer: 第59回日本生物物理学会年会
• [Presentation] ES 細胞のクロマチンドメイン変化による染色体動態制御のモデル (2021)
  • Author(s): 小本 哲史, 藤井 雅史, 粟津 暁紀
  • Organizer: 第59回日本生物物理学会年会
• [Book] Experimental Approaches of NMR Spectroscopy II (2025)
  • Author(s): Shin-ichi Tate (Chapter)
  • Total Pages: 411
  • Publisher: Springer Nature
  • ISBN: 9789819768332
• [Remarks] Shin-ichi Tate Lab.
  • URL: https://www.mls.sci.hiroshima-u.ac.jp/biophys/index.html
• [Remarks] Awazu Lab.
  • URL: http://www.math.sci.hiroshima-u.ac.jp/awa/
• [Remarks]
  • URL: https://www.mls.sci.hiroshima-u.ac.jp/biophys/index.html
• [Remarks] Molecular Biophysics Laboratory
• [Funded Workshop] Taiwan-Japan NMR Symposium (2024)