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Non-equilibrium mechanics of cellular symmetry and migratory principle by active gel physics

Research Project

Project/Area Number 21K18605
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 13:Condensed matter physics and related fields
Research InstitutionKyushu University

Principal Investigator

Maeda Yusuke  九州大学, 理学研究院, 准教授 (30557210)

Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2021: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Keywordsアクティブマター / 細胞骨格 / 人工細胞 / アクトミオシン / マイクロ流体デバイス / 非平衡物理学 / 合成生物学 / 秩序形成 / 分子モーター / 実験
Outline of Research at the Start

細胞はエネルギーを消費し,秩序だった構造を維持しながら,動き,変形する複雑な分子システムである。このようなダイナミクスの根幹となる概念が「対称性」である。生命の基本単位である細胞の対称性も,細胞運動や細胞分裂,さらには分化する細胞の運命をも決定する。しかし,細胞内での構造配置の対称性がどのように制御され,細胞の自発的な運動が誘起されるのか、その基本原理は十分理解されていない。この問題を解決するために本研究では,細胞内環境の複雑性を軽減した人工細胞モデルを確立し,細胞のように自律的に動き,変形する人工細胞の運動原理を解明する。本研究から細胞の動きと変形の普遍法則に挑む技術基盤が確立される。

Outline of Final Research Achievements

Cells are complex molecular systems that can move and deform spontaneously, converting chemical energy into mechanical action. To do this, intracellular symmetry plays a key role in determining the axes of cell motility and division. In this study, we developed an artificial cell model that encapsulates a cell extract system containing the cytoskeletal protein actin and myosin molecular motor in cell-sized microdroplets to elucidate how intracellular symmetry is determined and how spontaneous cell migration and deformation arise.
We found an artificial cell that moves and deforms autonomously under mechanical constraints, just like a living cell. The periodic actin waves generated in the artificial cells can drive this migration, and the active gel theory developed in this study can explain the onset of such actin wave formation in a cell-sized space. This finding paves the way for understanding the mechanics of cell migration underlying dynamic intracellular symmetry.

Academic Significance and Societal Importance of the Research Achievements

細胞運動は発生過程からガン細胞の転移に至る多くの生命現象に関わっている.本研究で構築した「運動する人工細胞」はそのシンプルさゆえに詳細な理解を可能としている.そのため, 人工細胞のモデル系を実験物理学の対象として確立することは,従来は隔たりがあったソフトマター物理学・細胞生物学・合成生物学を融合し,細胞内対称性の力学的理解と自在な制御の基礎となるものである.将来的には生体内を動く細胞や培養細胞の解析に応用することで,ガン細胞の浸潤を予測する医療シミュレーターなどの解析手法の創出につながると期待できる.

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (22 results)

All 2024 2023 2022 2021

All Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results)

  • [Journal Article] Unveiling the physics underlying symmetry breaking of the actin cytoskeleton: An artificial cell-based approach2023

    • Author(s)
      Sakamoto Ryota、Maeda Yusuke T.
    • Journal Title

      Biophysics and Physicobiology

      Volume: 20 Issue: 3 Pages: n/a

    • DOI

      10.2142/biophysico.bppb-v20.0032

    • ISSN
      2189-4779
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Physical Principle of Cell Migration Under Confinement: Artificial Cell-based Bottom-up Approach2023

    • Author(s)
      SAKAMOTO Ryota
    • Journal Title

      Seibutsu Butsuri

      Volume: 63 Issue: 3 Pages: 163-164

    • DOI

      10.2142/biophys.63.163

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] Building the Chromosome Segregation Apparatus2023

    • Author(s)
      SHIMAMOTO Yuta、MAEDA Yusuke T.、SAITO Kei
    • Journal Title

      Seibutsu Butsuri

      Volume: 63 Issue: 4 Pages: 193-195

    • DOI

      10.2142/biophys.63.193

    • ISSN
      0582-4052, 1347-4219
    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] State transitions of a confined actomyosin system controlled through contractility and polymerization rate2023

    • Author(s)
      Sakamoto Ryota、Miyazaki Makito、Maeda Yusuke T.
    • Journal Title

      Physical Review Research

      Volume: 5 Issue: 1 Pages: 013208-013208

    • DOI

      10.1103/physrevresearch.5.013208

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Geometric trade-off between contractile force and viscous drag determines the actomyosin-based motility of a cell-sized droplet2022

    • Author(s)
      Sakamoto Ryota、Izri Ziane、Shimamoto Yuta、Miyazaki Makito、Maeda Yusuke T.
    • Journal Title

      Proceedings of the National Academy of Sciences USA

      Volume: 119 Issue: 30

    • DOI

      10.1073/pnas.2121147119

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Morphological growth dynamics, mechanical stability, and active microtubule mechanics underlying spindle self-organization2022

    • Author(s)
      Fukuyama Tatsuya、Yan Lucan、Tanaka Masahito、Yamaoka Megumi、Saito Kei、Ti Shih-Chieh、Liao Chung-Chi、Hsia Kuo-Chiang、Maeda Yusuke T.、Shimamoto Yuta
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 44

    • DOI

      10.1073/pnas.2209053119

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Collective motion of epithelial cells along a wrinkled 3D-buckled hydrogel2022

    • Author(s)
      Shigeta Kazuyuki、Fukuyama Tatsuya、Takahashi Riku、Beppu Kazusa、Tanaka Aya、Maeda Yusuke T.
    • Journal Title

      RSC Advances

      Volume: 12 Issue: 31 Pages: 20174-20181

    • DOI

      10.1039/d2ra01768g

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Phase Separation and Protein Partitioning in Compartmentalized Cell-Free Expression Reactions2021

    • Author(s)
      Kato Shuzo、Garenne David、Noireaux Vincent、Maeda Yusuke T.
    • Journal Title

      Biomacromolecules

      Volume: 22 Issue: 8 Pages: 3451-3459

    • DOI

      10.1021/acs.biomac.1c00546

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Edge current and pairing order transition in chiral bacterial vortices2021

    • Author(s)
      Beppu Kazusa、Izri Ziane、Sato Tasuku、Yamanishi Yoko、Sumino Yutaka、Maeda Yusuke T.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 39

    • DOI

      10.1073/pnas.2107461118

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Controlling Collective Motion of Kinesin-Driven Microtubules via Patterning of Topographic Landscapes2021

    • Author(s)
      Araki Shunya、Beppu Kazusa、Kabir Arif Md. Rashedul、Kakugo Akira、Maeda Yusuke T.
    • Journal Title

      Nano Letters

      Volume: 21 Issue: 24 Pages: 10478-10485

    • DOI

      10.1021/acs.nanolett.1c03952

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 細胞核はどこにあるか - アクティブ・ゲルと配置対称性の制御原理2021

    • Author(s)
      坂本遼太, 前多裕介, 宮﨑牧人
    • Journal Title

      日本物理学会誌

      Volume: 76 Pages: 595-600

    • NAID

      130008084781

    • Related Report
      2021 Research-status Report
  • [Journal Article] ソフトマターで分子を運ぶ, 選り分ける2021

    • Author(s)
      前多裕介, 加藤修三, 福山達也
    • Journal Title

      現代化学

      Volume: 607 Pages: 46-49

    • Related Report
      2021 Research-status Report
  • [Presentation] Effect of the confining geometry on self-organization of active cytoskeleton2024

    • Author(s)
      Archit Negi, Ryota Sakamoto, Yusuke T. Maeda
    • Organizer
      日本物理学会2024年春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] State transitions and non-equilibrium contractile dynamics in active cytoskeletons2023

    • Author(s)
      柏原智香, 前多裕介
    • Organizer
      第61回日本生物物理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Geometry-induced dynamics of confined chiral active matter2023

    • Author(s)
      Archit Negi, Kazusa Beppu, Yusuke T. Maeda
    • Organizer
      Gordon conference: Soft matter far from equilibrium
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Geometry-induced dynamics of confined chiral active matter2023

    • Author(s)
      Archit Negi, Kazusa Beppu, Yusuke T. Maeda
    • Organizer
      Statphys28
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cargo transportation by kinesin-1 on collectively aligned microtubule2023

    • Author(s)
      Tomoka Kashiwabara, Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, Akira Kakugo, Yusuke T. Maeda
    • Organizer
      Statphys28
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 力が制御するアクトミオシンの秩序転移と自律運動・変形2022

    • Author(s)
      前多 裕介
    • Organizer
      第11回分子モーター討論会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] Understanding the instability of intracellular organization in synthetic cell2021

    • Author(s)
      前多裕介
    • Organizer
      第59回 日本生物物理学会年会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] アクティブな界面摩擦と流体抵抗の幾何学的バランスが決めるアクトミオシン液滴の自発運動2021

    • Author(s)
      坂本 遼太, Ziane Izri, 島本勇太, 宮﨑 牧人, 前多 裕介
    • Organizer
      第59回 日本生物物理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] アクティブゲルの対称性と動きの非平衡力学2021

    • Author(s)
      前多裕介
    • Organizer
      日本物理学会2021年度秋季大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] アクティブマター:生命システムの理解と制御の新展開2021

    • Author(s)
      前多裕介
    • Organizer
      東京大学化学生命工学専攻 談話会
    • Related Report
      2021 Research-status Report
    • Invited

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Published: 2021-07-13   Modified: 2025-01-30  

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