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Exploration of the modification of genomic higher-order structures in the cell nucleus by aggregation of chemically synthesized short nucleic acids

Research Project

Project/Area Number 21K19040
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 37:Biomolecular chemistry and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Okamoto Akimitsu  東京大学, 大学院工学系研究科(工学部), 教授 (60314233)

Project Period (FY) 2021-07-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2022: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2021: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords核酸 / 化学合成 / 集合体 / ハイブリダイゼーション / マイクロRNA / 相分離 / 転写因子
Outline of Research at the Start

化学合成短鎖核酸は細胞に入り込むと速やかに細胞核へ移動して、核内で蓄積する。蓄積した核酸が集合体を作る場合、その集合体が一定の濃度で結合タンパク質を呼び寄せることができれば、相分離を引き起こして液滴状顆粒を形成するかもしれない。核内DNA結合性タンパク質のトラップによる「DNA不溶化」による強制的な液滴状顆粒の形成は、核膜内膜領域の「流動性」「粘度」「屈折率」を局所的に大きく変える可能性がある。ヘテロクロマチンが形成するコンパートメントと融合しない液滴になった場合には、ゲノム高次構造に大きな揺らぎを与えて、核膜内膜周縁部の物資輸送やゲノムのトポロジカル関連ドメインに改変が起こるかもしれない。

Outline of Final Research Achievements

We have created a pair of chemically synthesized hairpin nucleic acids with sequences that form assemblies by sequential hybridization. In vitro experiments confirmed that these nucleic acid pairs form aggregates starting from miRNAs. When the nucleic acid pairs were added to HeLa cells together with a cell transfection agent, the nucleic acid pairs formed aggregates in the cytoplasm starting from miRNAs. Observation by fluorescence microscopy of FRET of the fluorescent labeling of the nucleic acids showed that they caused phase separation in the cytoplasm and formed droplet-like granules. Furthermore, FACS analysis revealed that strong FRET-derived fluorescence appeared in cells overexpressing the target miR-21, whereas FRET-derived fluorescence was weak in cells with low miR-21 expression.

Academic Significance and Societal Importance of the Research Achievements

今回の研究成果として、ヘアピン型人工核酸が細胞質内で相分離を引き起こすことやその原因が自然免疫関連タンパク質cGASの結合によること、さらにはその複合体形成が細胞死へ至らしめることが明らかになり、新たな部類の核酸医薬品のタネを見つけることができた。将来的には、化学合成ヘアピン型核酸対についてさらに検討を加えることによって他のタンパク質をトラップして効率的にノックダウンできる相分離系を作成したい。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (58 results)

All 2023 2022 2021

All Journal Article (15 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 15 results,  Open Access: 3 results) Presentation (43 results) (of which Int'l Joint Research: 7 results)

  • [Journal Article] Oncolytic Hairpin DNA Pair: Selective Cytotoxic Inducer through MicroRNA-Triggered DNA Self-Assembly2023

    • Author(s)
      Morihiro, K.; Osumi, H.; Morita, S.; Hattori, T.; Baba, M.; Harada, N.; Ohashi, R.; Okamoto, A.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 145 Issue: 1 Pages: 135-142

    • DOI

      10.1021/jacs.2c08974

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interstrand Crosslinking Oligonucleotides Elucidate the Effect of Metal Ions on the Methylation Status of Repetitive DNA Elements2023

    • Author(s)
      Liu, S.; Morihiro, K.; Takeuchi, F.; Li, Y.; Okamoto, A.
    • Journal Title

      Front. Chem.

      Volume: 11 Pages: 1122474-1122474

    • DOI

      10.3389/fchem.2023.1122474

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Glutamine deficiency in solid tumor cells confers resistance to ribosomal RNA synthesis inhibitors2022

    • Author(s)
      Melvin Pan、Christiane Zorbas、Maki Sugaya、Kensuke Ishiguro、Miki Kato、Miyuki Nishida、Hai-Feng Zhang、Marco M. Candeias、Akimitsu Okamoto、Takamasa Ishikawa、Tomoyoshi Soga、Hiroyuki Aburatani、Juro Sakai、Yoshihiro Matsumura、Tsutomu Suzuki、Christopher G. Proud、Denis L. J. Lafontaine、Tsuyoshi Osawa
    • Journal Title

      Nature Communications

      Volume: 13(1) Issue: 1 Pages: 3706-3706

    • DOI

      10.1038/s41467-022-31418-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Photoactivatable Materials for Versatile Single-Cell Patterning Based on the Photocaging of Cell-Anchoring Moieties through Lipid Self-Assembly2022

    • Author(s)
      Yamahira Shinya、Misawa Ryuji、Kosaka Takahiro、Tan Mondong、Izuta Shin、Yamashita Hayato、Heike Yuji、Okamoto Akimitsu、Nagamune Teruyuki、Yamaguchi Satoshi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 144 Issue: 29 Pages: 13154-13162

    • DOI

      10.1021/jacs.2c02949

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Repetitive Thiazolidine Deprotection Using a Thioester-Compatible Aldehyde Scavenger for One-Pot Multiple Peptide Ligation.2022

    • Author(s)
      Nakatsu, K.; Okamoto, A.; Hayashi, G.; Murakami, H.
    • Journal Title

      Angewandte Chemie International Edition

      Volume: 61 Issue: 39

    • DOI

      10.1002/anie.202206240

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemoenzymatic labeling to visualize intercellular contacts using lipidated sortase A2022

    • Author(s)
      Yamaguchi, S.; Ikeda, R.; Umeda, Y.; Kosaka, T.; Yamahira, S.; Okamoto, A.
    • Journal Title

      ChemBioChem

      Volume: 23 Issue: 21

    • DOI

      10.1002/cbic.202200474

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioorthogonal Photoreactive Surfaces for Single-Cell Analysis of Intercellular Communications2022

    • Author(s)
      Kosaka Takahiro、Yamaguchi Satoshi、Izuta Shin、Yamahira Shinya、Shibasaki Yoshikazu、Tateno Hiroaki、Okamoto Akimitsu
    • Journal Title

      Journal of the American Chemical Society

      Volume: 144 Issue: 39 Pages: 17980-17988

    • DOI

      10.1021/jacs.2c07321

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular Protein Photoactivation Using Sterically Bulky Caging2022

    • Author(s)
      Yamaguchi, S.; Yamamoto, K.; Yamamoto, R.; Takamori, S.; Ishiwatari, A.; Minamihata, K.; Nagamune, T.; Okamoto, A.
    • Journal Title

      ChemBioChem

      Volume: 23 Issue: 22

    • DOI

      10.1002/cbic.202200476

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] cIAP1-based degraders induce degradation via branched ubiquitin architectures2022

    • Author(s)
      Akizuki Yoshino、Morita Mai、Mori Yuki、Kaiho-Soma Ai、Dixit Shivani、Endo Akinori、Shimogawa Marie、Hayashi Gosuke、Naito Mikihiko、Okamoto Akimitsu、Tanaka Keiji、Saeki Yasushi、Ohtake Fumiaki
    • Journal Title

      Nature Chemical Biology

      Volume: 19 Issue: 3 Pages: 311-322

    • DOI

      10.1038/s41589-022-01178-1

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silyl-Protected Propargyl Glycine for Multiple Labeling of Peptides by Chemoselective Silyl-Deprotection2021

    • Author(s)
      Kamo, N.; Hayashi, G.; Okamoto, A.
    • Journal Title

      Tetrahedron Lett.

      Volume: 73 Pages: 153093-153093

    • DOI

      10.1016/j.tetlet.2021.153093

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Poly(A)+ Sensing of Hybridization-Sensitive Fluorescent Oligonucleotide Probe Characterized by Fluorescence Correlation Methods2021

    • Author(s)
      Paulson, B.; Shin, Y.; Okamoto, A.; Oh, Y.-M.; Kim, J. K.; Pack, C.-G.
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 22 Issue: 12 Pages: 6433-6433

    • DOI

      10.3390/ijms22126433

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Base-Resolution Analysis of 5-Hydroxymethylcytidine by Selective Oxidation and Reverse Transcription Arrest2021

    • Author(s)
      Koyama, K.; Hayashi, G.; Ueda, H.; Ota, S.; Nagae, G.; Aburatani, H.; Okamoto, A.
    • Journal Title

      Org. Biomol. Chem.

      Volume: 19 Issue: 29 Pages: 6478-6486

    • DOI

      10.1039/d1ob00995h

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sterically Bulky Caging of Transferrin for Photoactivatable Intracellular Delivery2021

    • Author(s)
      Yamaguchi, S.; Takamori, S.; Yamamoto, K.; Ishiwatari, A.; Minamihata, K.; Yamada, E.; Okamoto, A.; Nagamune, T.
    • Journal Title

      Bioconjugate Chem.

      Volume: 32 Issue: 8 Pages: 1535-1540

    • DOI

      10.1021/acs.bioconjchem.1c00159

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] anti-syn Unnatural Base Pair Enables Alphabet-Expanded DNA Self-Assembly2021

    • Author(s)
      Morihiro, K.; Moriyama, Y.; Nemoto, Y.; Osumi, H.; Okamoto, A.
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 143 Issue: 35 Pages: 14207-14217

    • DOI

      10.1021/jacs.1c05393

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Changes of C≡C Triple Bond Vibration that Disclosed Non-Canonical Cytosine Protonation in i-Motif-Forming Oligodeoxynucleotides2021

    • Author(s)
      Itaya, R.; Idei, W.; Nakamura, T.; Nishihara, T.; Kurihara, R.; Okamoto, A.; Tanabe, K.
    • Journal Title

      ACS Omega

      Volume: 6 Issue: 47 Pages: 31595-31604

    • DOI

      10.1021/acsomega.1c04074

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] 光応答性細胞固定化表面を用いた免疫細胞の1細胞傷害性解析2023

    • Author(s)
      山口 哲志、小阪 高広、山平 真也、岡本 晃充
    • Organizer
      高分子学会 第32回バイオ高分子シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] RNAヘアピン技術によるがん選択的な細胞毒性2023

    • Author(s)
      森田 駿人、森廣 邦彦、岡本 晃充
    • Organizer
      日本核酸医薬学会 第7回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] がん選択的な免疫療法を可能にするヘアピン型DNA医薬2023

    • Author(s)
      森廣 邦彦、大住 拓輝、岡本 晃充
    • Organizer
      日本核酸医薬学会 第7回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マイクロRNAが触媒する病変細胞選択的なDNAデコイ生成2023

    • Author(s)
      安田 壮秀、森廣 邦彦、岡本 晃充
    • Organizer
      日本核酸医薬学会 第7回年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 光分解性タンパク質-高分子ハイブリッド材料を用いた細胞のケーシング2023

    • Author(s)
      山口 哲志、中条 一貴、岡本 晃充
    • Organizer
      第71回高分子討論会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 画像解析による1細胞分取のための光応答性ゲル薄膜の開発2023

    • Author(s)
      李 雪陽、山口 哲志、上原 廉二朗、山平 真也、岡本 晃充
    • Organizer
      第71回高分子討論会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 嵩高い光分解性保護基を用いたタンパク質の細胞内光活性化2023

    • Author(s)
      山本 涼太郎、山口 哲志、山本 一穂、南畑 孝介、岡本 晃充
    • Organizer
      第71回高分子討論会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 腫瘍溶解性ヘアピン DNAペア:マイクロ RNA駆動型 DNA自己組織化を介した選択的細胞毒性誘導剤2023

    • Author(s)
      森廣 邦彦、大住 拓輝、岡本 晃充
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 連結方向切り替え可能な One-Potペプチド連結法によるタンパク質化学合成2023

    • Author(s)
      中津 幸輝、岡本 晃充、林 剛介、村上 裕
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 細胞内部の膜タンパク質の定量的1細胞解析法の開発2023

    • Author(s)
      梅田 侑生、山口 哲志、山平 真也、中村 元直、岡本 晃充
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] miRNA応答型低分子薬剤放出システム2023

    • Author(s)
      冨田 康弘、岡本 晃充、森廣 邦彦
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] RNAヘアピン技術によるがん選択的な細胞毒性2023

    • Author(s)
      森田 駿人、森廣 邦彦、岡本 晃充
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 難治性がんの効果的な化学療法に向けた多剤一体型ミニ核酸医薬の開発2023

    • Author(s)
      込山 凌成、森廣 邦彦、岡本 晃充
    • Organizer
      第16回バイオ関連化学シンポジウム
    • Related Report
      2022 Annual Research Report
  • [Presentation] 連結方向切り替え可能なone-potペプチド連結法を用いたタンパク質化学合成2023

    • Author(s)
      中津 幸輝、岡本 晃充、林 剛介、村上 裕
    • Organizer
      第12回CSJ化学フェスタ
    • Related Report
      2022 Annual Research Report
  • [Presentation] 弱酸性微小環境応答性人工核酸を用いた がん選択的DNAアプタマーの開発2023

    • Author(s)
      根本 祐衣、森廣 邦彦、岡本 晃充
    • Organizer
      第12回CSJ化学フェスタ
    • Related Report
      2022 Annual Research Report
  • [Presentation] がん関連マイクロRNAが触媒する腫瘍細胞選択的なデコイ核酸医薬生成2023

    • Author(s)
      安田 壮秀、森廣 邦彦、岡本 晃充
    • Organizer
      第12回CSJ化学フェスタ
    • Related Report
      2022 Annual Research Report
  • [Presentation] Evaluation of biochemical properties of nucleosome formed by short interspersed nuclear element2023

    • Author(s)
      Yijing Lin, Takafumi Furuhata, Akimitsu Okamoto
    • Organizer
      The 49th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] miR-21 detection technology based on HCR and ECHO probe2023

    • Author(s)
      Yunsong Xu, Kunihiko Morihiro, Akimitsu Okamoto
    • Organizer
      The 49th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of multidrug-integrated mini-nucleic acid drugs for effective chemotherapy of refractory cancer2023

    • Author(s)
      Ryosei Komiyama, Kunihiko Morihiro, Akimitsu Okamoto
    • Organizer
      The 49th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Activation of siRNA triggered by copper ion accumulated in cancer cells2023

    • Author(s)
      Yasuhiro Tomida, Honami Ando, Kunihiko Morihiro, Akimitsu Okamoto
    • Organizer
      The 49th International Symposium on Nucleic Acids Chemistry
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞間接触を可視化する化学酵素的標識法2023

    • Author(s)
      山口 哲志、池田 諒佑、岡本 晃充
    • Organizer
      化学工学会第88年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 細胞間相互作用を創出する生体直交的な光反応性表面2023

    • Author(s)
      山口 哲志、小阪 高広、山平 真也、岡本 晃充
    • Organizer
      化学工学会第88年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 画像観察に基づく1細胞選別のための光応答性細胞付着ゲル薄膜の開発2023

    • Author(s)
      李 雪陽、山口 哲志、岡本 晃充
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ユビキチン―リガンドキメラペプチドによる標的タンパク質分解2023

    • Author(s)
      宮本 丈太郎、古畑 隆史、岡本 晃充
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 嵩高いケージングを用いた細胞内でのタンパク質の光活性化2023

    • Author(s)
      山本 涼太郎、山口 哲志、岡本 晃充
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] がん細胞内に蓄積された銅イオンをトリガーとするsiRNAの活性化2023

    • Author(s)
      冨田 康弘、森廣 邦彦 、岡本 晃充
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 難治性がんの効果的な化学療法に向けた多剤一体型短鎖核酸医薬の開発2023

    • Author(s)
      込山 凌成、森廣 邦彦、岡本 晃充
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Enzyme mediated one-pot synthesis of branched polyubiquitin (K48/K63) chain via photo-induced stepwise synthesis2023

    • Author(s)
      Phebee Angeline Devadasan Racheal, Takafumi Furuhata1, Iori Murayama, Usano Toyoda, Akimitsu Okamato
    • Organizer
      日本化学会第103春季年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] がん診断に向けた多種マイクロRNAの同時蛍光検出2023

    • Author(s)
      徐 云嵩、森廣 邦彦、岡本 晃充
    • Organizer
      日本薬学会第143年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] マイクロRNAが触媒するデコイ核酸医薬の腫瘍細胞内生成2023

    • Author(s)
      安田 壮秀、森廣 邦彦、岡本 晃充
    • Organizer
      日本薬学会第143年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 白金系抗悪性腫瘍剤によるヌクレオソームの物理化学的変化と位置の再組織化2023

    • Author(s)
      廖 宣宇、古畑 隆史、岡本 晃充
    • Organizer
      日本薬学会第143年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 核酸ナノ技術によりがん選択的な細胞毒性を獲得するヘアピン型RNA分子の開発2023

    • Author(s)
      森田 駿人、森廣 邦彦、岡本 晃充
    • Organizer
      日本薬学会第143年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Tumor immune activation through intracellular hairpin DNA assenbly2023

    • Author(s)
      Kunihiko Morihiro, Hiraki Osumi, Akimitsu Okamoto
    • Organizer
      18th Annual Meeting of the Oligonucleotide Therapeutics Society
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Production of DNA decoy catalyzed with microDNA only in deseased cells2023

    • Author(s)
      Soshu Yasuda, Kunihiko Morihiro, Akimitsu Okamoto
    • Organizer
      18th Annual Meeting of the Oligonucleotide Therapeutics Society
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 光応答性基板を用いた免疫細胞の細胞傷害性のシングルセル解析2022

    • Author(s)
      小阪 高広、山口 哲志、山平 真也、岡本 晃充
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Enzymatic branched ubiquitin chain formation on K63 homotypic chain with branched points defined by photo-induced stepwise poly-ubiquitin synthesis2022

    • Author(s)
      Phebee Angeline Devadasan Racheal、Takafumi Furuhata、Iori Murayama、Usano Toyoda、Akimitsu Okamoto
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Evaluation of nucleosome structure alteration and accessibility change caused by platinum-based antineoplastics2022

    • Author(s)
      Xuanyu LIAO、Takafumi Furuhata、Akimitsu Okamoto、Ikuma Arakaki
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 低分子リガンド-ユビキチンキメラを用いたタンパク質の間接的ユビキチン化法2022

    • Author(s)
      宇野 大輝、藤田 涼香、古畑 隆史、岡本 晃充
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 刺激分解性ビオチン化試薬を用いた細胞内タンパク質送達2022

    • Author(s)
      山本 一穂、山口 哲志、岡本 晃充
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 2-aminobenzamide誘導体によるチアゾリジン開環とone-potペプチド連結法への応用2022

    • Author(s)
      中津 幸輝、村上 裕、林 剛介、岡本 晃充
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] ユビキチンキメラ分子による転写因子のケミカルノックダウン2022

    • Author(s)
      吉田 和生、藤田 涼香、古畑 隆史、岡本 晃充
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Indirect ubiquitination for targeted degradation of the transcription factor2022

    • Author(s)
      Ryoka Fujita、Takafumi Furuhata、Yuto Matsumura、Tokiha Ozawa、Akimitsu Okamoto
    • Organizer
      第48回国際核酸化学シンポジウム
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 細胞膜のマイクロ流体剥離技術を用いた細胞内1細胞解析2022

    • Author(s)
      山口 哲志・ 梅田 侑生・山平 真也・ 岡本 晃充
    • Organizer
      化学工学会第87年会
    • Related Report
      2021 Research-status Report

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Published: 2021-07-13   Modified: 2024-01-30  

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