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Development of Genomics and Chemical approach for the novel plant peptide signaling

Research Project

Project/Area Number 21K19074
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionNagoya University

Principal Investigator

Tabata Ryo  名古屋大学, 生命農学研究科, 特任講師 (30712294)

Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2022: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2021: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords植物 / ペプチド / 短鎖翻訳後修飾型ペプチド / システインリッチペプチド / 環境ストレス応答 / 分泌型ペプチド / 器官間シグナル / 低酸素ストレス
Outline of Research at the Start

脳や神経系に相当する器官を持たない植物は、維管束を介して「根と葉」の間の器官間コミュニケーションの仕組みを発達させ、個体を統御するシステムを進化させてきた。近年、植物はペプチド分子による器官間シグナル伝達機構を介して個体のホメオスタシスを維持している事が明らかになってきたが、植物のゲノムには約1000 個の分泌型ペプチド遺伝子が存在しており、機能解明された因子は限られている。そこで本研究では、環境ストレスに応答した器官間シグナル伝達機構を支える器官間移動性のペプチド分子に着目して、化学的・ゲノム科学の研究アプローチによってストレス応答に必須の新規ペプチドシグナリングを探索する。

Outline of Final Research Achievements

Small secreted peptides are important for plant development and environmental stress response. It is estimated that more than 1000 potential secreted peptides exist in Arabidopsis genome, however, so far only very few have been functionally characterized because of the difficulty in elucidating their mature peptide structures and gene redundancy. In this study, I identified the tomato tyrosine-sulfated peptide through a xylem sap peptidomics approach. In addition, I also performed genomics approach (CRISPR/Cas9 genome-editing technique in the ku70 or ligase4-deficient mutant background) and found novel cysteine-rich peptides are essential for Hypoxic stress Response. These analyses may pave the way for understanding the novel plant peptide signaling.

Academic Significance and Societal Importance of the Research Achievements

今回の新規ペプチド分子の発見は、植物の細胞間・器官間シグナルの解明へ向けた手がかりであり、環境変動に適応する植物の巧みな生存戦略を理解するうえで非常に重要である。また、ストレス応答を制御するペプチド分子を活用することができれば、分解され無害なアミノ酸に還るペプチドを利用して、ストレス耐性を強化することが期待され、効率的な作物栽培のための基盤技術創成に繋がると考えられる。また、植物の根と葉のコミュニケーションを制御可能なペプチド分子を見出し、活用することができれば、「葉」にペプチドを塗布することで「根」でのストレス応答を向上させるなど、新しい栽培制御技術の創出にもつなげることができる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (18 results)

All 2023 2022 2021

All Journal Article (8 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 8 results,  Open Access: 6 results) Presentation (10 results) (of which Invited: 2 results)

  • [Journal Article] Regulation of the iron-deficiency response by IMA/FEP peptide2023

    • Author(s)
      Tabata Ryo
    • Journal Title

      Frontiers in Plant Science

      Volume: 14 Pages: 1107405-1107405

    • DOI

      10.3389/fpls.2023.1107405

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PECT1, a rate‐limiting enzyme in phosphatidylethanolamine biosynthesis, is involved in the regulation of stomatal movement in Arabidopsis2023

    • Author(s)
      Negi Juntaro、Obata Tomoki、Nishimura Sakura、Song Boseok、Yamagaki Sho、Ono Yuhei、Okabe Makoto、Hoshino Natsumi、Fukatsu Kohei、Tabata Ryo、Yamaguchi Katsushi、Shigenobu Shuji、Yamada Masashi、Hasebe Mitsuyasu、Sawa Shinichiro、Kinoshita Toshinori、Nishida Ikuo、Iba Koh
    • Journal Title

      The Plant Journal

      Volume: - Issue: 2 Pages: 563-576

    • DOI

      10.1111/tpj.16245

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nicotiana benthamiana XYLEM CYSTEINE PROTEASE genes facilitate tracheary element formation in interfamily grafting2023

    • Author(s)
      Huang Chaokun、Kurotani Ken-ichi、Tabata Ryo、Mitsuda Nobutaka、Sugita Ryohei、Tanoi Keitaro、Notaguchi Michitaka
    • Journal Title

      Horticulture Research

      Volume: - Issue: 6 Pages: 1-12

    • DOI

      10.1093/hr/uhad072

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] NAC103 mutation alleviates DNA damage in an Arabidopsis thaliana mutant sensitive to excess boron2023

    • Author(s)
      Sotta Naoyuki、Sakamoto Takuya、Kamiya Takehiro、Tabata Ryo、Yamaguchi Katsushi、Shigenobu Shuji、Yamada Masashi、Hasebe Mitsuyasu、Sawa Shinichiro、Fujiwara Toru
    • Journal Title

      Frontiers in Plant Science

      Volume: 14 Pages: 1099816-1099816

    • DOI

      10.3389/fpls.2023.1099816

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Analysis of plasmodesmata permeability using cultured tobacco BY-2 cells entrapped in microfluidic chips2022

    • Author(s)
      Kurotani Ken-ichi、Kawakatsu Yaichi、Kikkawa Masahiro、Tabata Ryo、Kurihara Daisuke、Honda Hiroyuki、Shimizu Kazunori、Notaguchi Michitaka
    • Journal Title

      Journal of Plant Research

      Volume: 135 Issue: 5 Pages: 693-701

    • DOI

      10.1007/s10265-022-01406-8

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Systemic Regulation of Iron Acquisition by Arabidopsis in Environments with Heterogeneous Iron Distributions2022

    • Author(s)
      Ryo Tabata, Takehiro Kamiya, Shunpei Imoto, Hana Tamura, Michika Tabata, Tasuku Hirayama, Hironaka Tsukagoshi, Keitaro Tanoi, Takamasa Suzuki, Takushi Hachiya, Hitoshi Sakakibara
    • Journal Title

      Plant Cell Physiology

      Volume: - Issue: 6 Pages: 842-854

    • DOI

      10.1093/pcp/pcac049

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of microfluidic chip for entrapping tobacco BY-2 cells2022

    • Author(s)
      Kazunori Shimizu, Yaichi Kawakatsu, Ken-ichi Kurotani, Masahiro Kikkawa, Ryo Tabata, Daisuke Kurihara, Hiroyuki Honda, Michitaka Notaguchi
    • Journal Title

      PLoS ONE

      Volume: - Issue: 4 Pages: e0266982-e0266982

    • DOI

      10.1371/journal.pone.0266982

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regulation of ammonium acquisition and use in Oryza longistaminata ramets under nitrogen source heterogeneity2022

    • Author(s)
      Misato Kawai, Ryo Tabata, Miwa Ohashi, Haruno Honda, Takehiro Kamiya, Mikiko Kojima, Yumiko Takebayashi, Shunsuke Oishi, Satoru Okamoto, Takushi Hachiya, Hitoshi Sakakibara
    • Journal Title

      Plant Physiology

      Volume: 188 Issue: 4 Pages: 2364-2376

    • DOI

      10.1093/plphys/kiac025

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 鉄欠乏ストレス応答性分泌型システインリッチペプチドの機能解析2023

    • Author(s)
      和久茉厘那, 井本駿平, 神谷岳洋, 菅野茂夫, 戸田陽介, 中道範人, 木羽隆敏, 榊原均, 田畑亮
    • Organizer
      日本土壌肥料学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] シロイヌナズナの器官間シグナルを介した鉄欠乏応答制御2023

    • Author(s)
      塩谷太一, 井本駿平, 神谷岳洋, 木羽隆敏, 榊原均, 田畑亮
    • Organizer
      日本土壌肥料学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Understanding the role of de novo tracheary elements in Nicotiana interfamily grafting2023

    • Author(s)
      Huang Chaokun、Kurotani Ken-ichi、Tabata Ryo、Mitsuda Nobutaka、Sugita Ryohei、Tanoi Keitaro、Notaguchi Michitaka
    • Organizer
      第64回 日本植物生理学会年会
    • Related Report
      2022 Research-status Report
  • [Presentation] シロイヌナズナIMA/FEPを介した器官間シグナルによる鉄吸収制御2022

    • Author(s)
      田畑亮、神谷岳洋、井本駿平、木羽隆敏、榊原均
    • Organizer
      日本土壌肥料学会 2022年度東京大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 接木後の組織修復におけるオートファジー誘導の発見2022

    • Author(s)
      黒谷賢一, 田畑亮, 川勝弥一, 杉田亮平, 岡安浩次, 田野井慶太郎, 野田口理孝
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] Xylem formation enhances scion growth of Nicotiana interfamily grafting2022

    • Author(s)
      Chaokun Huang, Kenichi Kurotani, Ryo Tabata, Michitaka Notaguchi
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Research-status Report
  • [Presentation] 植物の器官間シグナル伝達を介した鉄吸収制御2021

    • Author(s)
      田畑亮
    • Organizer
      第255回遺伝子機能解析部門セミナー 島根大学
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] シロイヌナズナ器官間シグナリングを介した「鉄」吸収制御2021

    • Author(s)
      田畑亮
    • Organizer
      植物の栄養研究会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] シロイヌナズナにおける器官間シグナル伝達を介した鉄吸収制御2021

    • Author(s)
      田畑亮, 井本駿平, 田村花, 神谷岳洋, 木羽隆敏, 榊原均
    • Organizer
      日本土壌肥料学会2021年度北海道大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Regeneration of tracheary elements facilitate scion growth upon N. benthamiana interfamily grafting2021

    • Author(s)
      Chaokun Huang, Michitaka Notaguchi, Kenichi Kurotani, Yaichi Kawakatsu, Ryo Tabata
    • Organizer
      第85回 日本植物学会年会
    • Related Report
      2021 Research-status Report

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Published: 2021-07-13   Modified: 2025-01-30  

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