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Development of continuous microbial-lipid and functional material production from defatted rice bran by using environmentally harmonized process.

Research Project

Project/Area Number 21K19159
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 41:Agricultural economics and rural sociology, agricultural engineering, and related fields
Research InstitutionYamagata University

Principal Investigator

WATANABE Masanori  山形大学, 農学部, 教授 (20320020)

Co-Investigator(Kenkyū-buntansha) 山田 千佳子  名古屋学芸大学, 管理栄養学部, 准教授 (30351216)
Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Keywords脱脂米糠 / 脱脂米糠糖化液 / 微生物油脂 / 油性酵母 / PCA解析 / 油脂生産酵母 / 米糠タンパク質 / 有機リン化合物 / アレルゲンフリータンパク質 / 米由来バイオマス
Outline of Research at the Start

世界的な食料需要の増加,バイオ燃料等の油脂需要構造の変化により,現在,タンパク質・油脂の安定供給が望まれている。本研究課題では,米油製造後に副生される脱脂米糠からアレルゲンフリータンパク質・有機リン化合物の回収・精製とその残渣からの効率的な機能性油脂生産の双方を可能にする完全資源化・ゼロエミッション技術を開発する。

Outline of Final Research Achievements

In order to understand food science properties of highly concentrated proteins obtained from defatted rice bran by the IP-EWT method and the production of microbial lipid from C5 and C6 sugars contained in the residue fraction showed that the oil and water absorption properties and emulsifying activity of rice bran proteins tended to be comparable to those of casein proteins. The results showed that the oil- and water-absorption properties and emulsification activity values of rice bran protein were similar to those of casein. Analysis of dynamic viscoelastic properties showed that storage elasticity exceeded loss elasticity, indicating the ability to form stable gels. Results of Principal component analysis (PCA) and Biplot analysis of microbial lipid production test under arbitrary control of both C:N and C:P ratios of culture medium indicated the possibility of high TUD oil production by suppressing cell growth, although the amount of oil production was reduced.

Academic Significance and Societal Importance of the Research Achievements

本研究課題は,未利用バイオマスである米加工副産物(脱脂米糠)の新規再資源化技術開発を試みるものであり,脱脂米糠からのアレルゲンフリータンパク質,有機リン化合物の生成のみならず,その残渣から微生物油脂を生成することにより,脱脂米糠の完全資源化を目指す点に最大の特徴がある。本研究の実施により,油糧作物以外の未利用植物性バイオマスからの油脂生産に関わる基盤技術の確立の他,ゼロエミッションへの貢献をも可能にするものであり,本研究の進展により,バイオリファイナリー技術開発にイノベーション,他のバイオマスへの応用展開,SDGsへの貢献も期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (21 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (11 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 11 results,  Open Access: 11 results) Presentation (4 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Chiang Mai University(タイ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Chiang Mai University(タイ)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] Chiang Mai University(タイ)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Development of New Properties and Market Introduction of Allergen-free Protein and Functional Organic Phosphorus Compound as Food Law Materials Derived from Defatted Rice Bran by Using Environmental Harmonized Process: Possibility of Sustainable Rice Cropping by the Plant Base Protein Production from Locally Produced Paddy Rice2023

    • Author(s)
      WATANABE Masanori
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 61 Issue: 4 Pages: 172-178

    • DOI

      10.1271/kagakutoseibutsu.61.172

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2023-04-01
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Kinetics of Phosphate Ions and Phytase Activity Production for Lactic Acid-Producing Bacteria Utilizing Milling and Whitening Stages Rice Bran as Biopolymer Substrates2023

    • Author(s)
      Nunta Rojarej、Khemacheewakul Julaluk、Techapun Charin、Sommanee Sumeth、Feng Juan、Htike Su Lwin、Mahakuntha Chatchadaporn、Porninta Kritsadaporn、Phimolsiripol Yuthana、Jantanasakulwong Kittisak、Moukamnerd Churairat、Watanabe Masanori、Kumar Anbarasu、Leksawasdi Noppol
    • Journal Title

      Biomolecules

      Volume: 13 Issue: 12 Pages: 1770-1770

    • DOI

      10.3390/biom13121770

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of a High-Quality/Yield Long-Read Sequencing-Adaptable DNA Extraction Method for Crop Seeds2023

    • Author(s)
      Shioya Naohiro、Ogiso-Tanaka Eri、Watanabe Masanori、Anai Toyoaki、Hoshino Tomoki
    • Journal Title

      Plants

      Volume: 12 Issue: 16 Pages: 2971-2971

    • DOI

      10.3390/plants12162971

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of newly isolated thermotolerant bacterium Cupriavidus sp. CB15 from composting and its ability to produce polyhydroxyalkanoate from glycerol2023

    • Author(s)
      Yootoum Anuyut、Jantanasakulwong Kittisak、Rachtanapun Pornchai、Moukamnerd Churairat、Chaiyaso Thanongsak、Pumas Chayakorn、Tanadchangsaeng Nuttapol、Watanabe Masanori、Fukui Toshiaki、Insomphun Chayatip
    • Journal Title

      Microbial Cell Factories

      Volume: 22 Issue: 1 Pages: 68-83

    • DOI

      10.1186/s12934-023-02059-5

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hydrothermal-enzymatic process for the bio-valorization of keratin wastes by thermostable keratinase from Thermoactinomyces vulgaris TK1-212023

    • Author(s)
      Tanyawat Kaewsalud, Kamon Yakul, Chayatip Insomphun, Kittisak Jantanasakulwong, Pornchai Rachtanapun, Wanaporn Tapingkae, Santi Chuetor, Masanori Watanabe, Thanongsak Chaiyaso
    • Journal Title

      Journal of Chemical Technology and Biotechnology

      Volume: 98 Issue: 5 Pages: 1203-1214

    • DOI

      10.1002/jctb.7330

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Boron Deficiency Enhances Microcracking in Tomato Fruit during Summer2022

    • Author(s)
      Gnon Bouanra Marietta Gonroudobou, Tomoyuki Nabeshima, Takashi Nishizawa and Masanori Watanabe
    • Journal Title

      Chiang Mai Journal of Science

      Volume: 49 Issue: 4 Pages: 1040-1049

    • DOI

      10.12982/cmjs.2022.072

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Development of Ethyl Methanesulfonate Mutant Edamame Soybean ( Glycine max (L.) Merr.) Populations and Forward and Reverse Genetic Screening for Early-Flowering Mutants2022

    • Author(s)
      Natsume Koshika, Naohiro Shioya, Takashi Fujimura, Rina Oguchi, Chie Ota, Emi Kato, Reiko Takahashi, Shuichi Kimura, Shinsuke Furuno, Koichi Saito, Kazuhiro Okabe, Masanori Watanabe and Tomoki Hoshino
    • Journal Title

      Plants

      Volume: 11 Issue: 14 Pages: 1839-1839

    • DOI

      10.3390/plants11141839

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of protease addition for reducing turbidity and flocculation of solid particles in drainage water derived from wheat-flour noodle boiling process and its electrostatic properties2021

    • Author(s)
      Watanabe Masanori、Chaiyaso Thanongsak、Techapun Charin、Shiono Tadahiko、Hoshino Tomoki、Nakamura Kozo、Takenaka Shinji、Isamu Maeda、Nabeshima Tomoyuki、Nishizawa Takashi
    • Journal Title

      Water Resources and Industry

      Volume: 25 Pages: 100150-100150

    • DOI

      10.1016/j.wri.2021.100150

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient Enzymatic Process for Mulberry Paper Production: An Approach for Xylooligosaccharide Production Coupled with Minimizing Bleaching Agent Doses2021

    • Author(s)
      Chaiyaso Thanongsak、Boonchuay Pinpanit、Takenaka Shinji、Techapun Charin、Rachtanapun Pornchai、Jantanasakulwong Kittisak、Watanabe Masanori
    • Journal Title

      Waste and Biomass Valorization

      Volume: open journal Issue: 10 Pages: 0-0

    • DOI

      10.1007/s12649-021-01416-y

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Prebiotic properties, antioxidant activity, and acute oral toxicity of xylooligosaccharides derived enzymatically from corncob2021

    • Author(s)
      Boonchuay Pinpanit、Wongpoomchai Rawiwan、Jaturasitha Sanchai、Mahatheeranont Sugunya、Watanabe Masanori、Chaiyaso Thanongsak
    • Journal Title

      Food Bioscience

      Volume: 40 Pages: 100895-100895

    • DOI

      10.1016/j.fbio.2021.100895

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Improvement of the halotolerance of a Bacillus serine protease by protein surface engineering2021

    • Author(s)
      Takenaka Shinji、Takada Airi、Kimura Yukihiro、Watanabe Masanori、Kuntiya Ampin
    • Journal Title

      Journal of Basic Microbiology

      Volume: 62 Issue: 2 Pages: 174-184

    • DOI

      10.1002/jobm.202100335

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Possibility of stable supply of rice-derived protein concentrate and development of plant-based meat2024

    • Author(s)
      Masanori WATANABE
    • Organizer
      The 7th international conference on food and applied bioscience 2024
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Physicochemical Property of Rice Bran Protein Derived from Heat-Stabilized Defatted Rice Bran.2022

    • Author(s)
      M. Watanabe, K. Ueda, C. Yamada, C. Techapun, N. Leksawasdi, T. Chaiyaso, S. Takenaka
    • Organizer
      International Conference on Food and Applied Bioscience (FAB 2022)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Microcracking in Tomato Fruit is Related to Epidermal Cell Division and Cell Enlargement2022

    • Author(s)
      Gnon Bouanra Marietta Gonroudobou, Tomoyuki Nabeshima, Takashi Nishizawa, Masanori Watanabe
    • Organizer
      International Conference on Food and Applied Bioscience (FAB 2022)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 脱脂米糠由来タンパク質の食品化学的特性の評価2022

    • Author(s)
      上田 恵子,廣瀨 明日香,遠藤 修二郎,山田 千佳子, 渡辺 昌規
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Research-status Report
  • [Book] 食品ロス削減と食品廃棄物資源化の技術 第IV編第9章 米加工副産物からの高純度タンパク質・リンの連続回収技術2023

    • Author(s)
      渡辺昌規
    • Total Pages
      9
    • Publisher
      シーエムシー出版
    • ISBN
      9784781317267
    • Related Report
      2022 Research-status Report
  • [Remarks] 山形大学農学部・渡辺研究室 バイオマス資源学研究室ホームページ

    • URL

      https://watanabelab.com/

    • Related Report
      2022 Research-status Report 2021 Research-status Report
  • [Patent(Industrial Property Rights)] 米糠の処理方法2022

    • Inventor(s)
      渡辺昌規
    • Industrial Property Rights Holder
      渡辺昌規
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-063261
    • Filing Date
      2022
    • Related Report
      2022 Research-status Report

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Published: 2021-07-13   Modified: 2025-01-30  

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