• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of odor sensing culture method for microalga Scenedesmus sp. (Chlorophyta) as novel agricultural crops

Research Project

Project/Area Number 21K19171
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 41:Agricultural economics and rural sociology, agricultural engineering, and related fields
Research InstitutionSaga University

Principal Investigator

Ueno Daisuke  佐賀大学, 農学部, 准教授 (60423604)

Project Period (FY) 2021-07-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2022: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2021: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Keywords微細藻類 / イカダモ / 分析化学 / におい嗅ぎガスクロマトグラフ / 匂い / におい嗅ぎガスクロマトグラフ(GC-O) / 栽培法 / 匂い物質 / GC-O
Outline of Research at the Start

本研究では微細藻類の“匂いセンシング技術”による培養法の開発に向け、佐賀産微細藻類の培養状態に応じた匂い成分を分析化学的に同定することを目的としている。佐賀産微細藻類の培養状態は,顕微鏡観察やセンサー類によって常時監視し、それぞれの各培養状態(増殖期、収穫適期、衰退期、異常時)の試料提供をうける.佐賀産微細藻類から発生する匂い物質は、匂い嗅ぎガスクロマトグラフィー(GC-O)、ガスクロマトグラフィー質量分析計(GC-MS)などをもちいて分離・同定する。本研究ではヒトの嗅覚をもちいて匂い成分を検出するGC-Oを利用しており、GC-MSだけでは検出できない微量の匂い成分の検出が可能である。

Outline of Final Research Achievements

Although several microalgae species have been used for industrial purposes, it is generally difficult to visually observe their growth conditions during cultivation. Our group has attempted to develop a way to control algae cultivation, using a method we refer to as “Odor Sensing Cultivation” (OSC). This is a new and simple observation method that is based on the odors produced by algae. In this study, as a representative microalgal species, Scenedesmus sp. was employed. Odors produced from its culture were investigated by olfactory evaluation, chemical identification. As a result of olfactory evaluation, “green tea”, “sweet”, and “fishy” were the characteristic odors identified from this species. In the chemical analysis, the odors from this species were identified as 1-nonanal, 2-undecenal, α-ionone, β-ionone, and tentatively identified as acetic acid. These results suggest that the OSC is applicable as a practical technique for production of this species.

Academic Significance and Societal Importance of the Research Achievements

嗅覚官能評価によるにおいの印象と理化学分析を併用することで,イカダモの収穫適期を把握できるだけでなく,細胞増殖の活性や健康状態,バクテリアの混入,他種の微細藻類の混入など,総合的な培養状態の変化を判別できる技術の可能性が示された.将来的に生産現場においてOSCを活用するためには,官能的/理化学的な指標に対する“判別基準”が必要となる.現在,生産現場と同等の大量培養条件の実験を計画しており,イカダモの多面的な培養状態の変化と,嗅覚官能評価および化学分析結果との関連性を検証し,OSCに向けた判別基準の策定が望まれる.

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (12 results)

All 2022 2021

All Journal Article (12 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 12 results,  Open Access: 9 results)

  • [Journal Article] Discrimination method of satsuma mandarin suffered by stored off-flavor (part 1) -identification of stored off-flavor compound-2022

    • Author(s)
      SASAGAWA Satoshi、KOTODA Nobuhiro、TANAKA Yoshiki、IKEDA Shigenori、MATSUMOTO Atsushi、SATO Katsuhisa、KAMIMURA Tomoko、KOYAMA Reon、UENO Daisuke
    • Journal Title

      Journal of Japan Association on Odor Environment

      Volume: 53 Issue: 6 Pages: 357-365

    • DOI

      10.2171/jao.53.357

    • ISSN
      1348-2904, 1349-7847
    • Year and Date
      2022-11-25
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sensory evaluation, chemical identification, and QCM-type e-nose analysis of odor compounds from a microalga <i>Scenedesmus</i> sp. (Chlorophyta) for development of odor sensing culture method2022

    • Author(s)
      KOYAMA Reon、DEMURA Mikihide、HASHIZUME Ken-Ichi、SEKINE Ayumi、SATO Katsuhisa、KAMIMURA Tomoko、SASAGAWA Satoshi、UENO Daisuke
    • Journal Title

      Journal of Japan Association on Odor Environment

      Volume: 53 Issue: 6 Pages: 345-356

    • DOI

      10.2171/jao.53.345

    • ISSN
      1348-2904, 1349-7847
    • Year and Date
      2022-11-25
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Beppu Bay, Japan, as a candidate Global Boundaries Stratotype Section and Point for an Anthropocene series2022

    • Author(s)
      Kuwae, M., Finney, B. P.,.. Hirose, K., Suzuki, H. and Saito, Y.
    • Journal Title

      Behavior of Radionuclides in the Environment

      Volume: 20530196221135077 Issue: 1 Pages: 1-38

    • DOI

      10.1177/20530196221135077

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comprehensive analyses of agrochemicals affecting aquatic ecosystems: A case study of Odonata communities and macrophytes in Saga Plain, northern Kyushu, Japan2022

    • Author(s)
      Tazunoki Yuhei、Tokuda Makoto、Sakuma Ayumi、Nishimuta Kou、Oba Yutaro、Kadokami Kiwao、Miyawaki Takashi、Ikegami Makihiko、Ueno Daisuke
    • Journal Title

      Environmental Pollution

      Volume: 292 Pages: 118334-118334

    • DOI

      10.1016/j.envpol.2021.118334

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Identification of aroma compounds from <i>Trentepohlia aurea</i> (Linnaeus) Martius using gas chromatography-olfactometry2021

    • Author(s)
      小山玲音, 出村幹英, 野間誠司, 林信行, 原口智和, 宮本英揮, 笹川智史, 龍田典子, 上野大介
    • Journal Title

      Journal of Japan Association on Odor Environment

      Volume: 52 Issue: 4 Pages: 226-232

    • DOI

      10.2171/jao.52.226

    • NAID

      130008116339

    • ISSN
      1348-2904, 1349-7847
    • Year and Date
      2021-07-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analytical method development of skin gas from beef cattle (Wagyu) using a gas chromatography-olfactometry2021

    • Author(s)
      松本英顕, 江原史雄, 小山玲音, 笹川智史, 原口智和, 宮本英揮, 龍田典子, 上野大介
    • Journal Title

      Journal of Japan Association on Odor Environment

      Volume: 52 Issue: 4 Pages: 233-239

    • DOI

      10.2171/jao.52.233

    • NAID

      130008116341

    • ISSN
      1348-2904, 1349-7847
    • Year and Date
      2021-07-25
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of Distinctive Odor Compounds Generated from Garlic Infected by Potato Rot Nematode Using Gas Chromatography-olfactometry2021

    • Author(s)
      古賀夕貴, 吉賀豊司, 新藤潤一, 青山理絵, 松本英顕, 龍田典子, 原口智和, 宮本英揮, 上野大介
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 70 Issue: 7.8 Pages: 427-434

    • DOI

      10.2116/bunsekikagaku.70.427

    • NAID

      130008101162

    • ISSN
      0525-1931
    • Year and Date
      2021-07-05
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Contaminants of emerging concern detected by comprehensive target analysis in a sediment core collected from Osaka Bay, Japan2021

    • Author(s)
      Nishimuta, K., Ueno, D., Takahashi, S., Kuwae, M., Tsugeki, N.K., Kadokami, K., Miyawaki, T., Matsukami, H., Kuramochi, H., Miyamoto, H., Haraguchi, T., Ryuda, N., Sakai, S.
    • Journal Title

      J. Pollut. Effects Control

      Volume: 9 Pages: 283-283

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Use of comprehensive target analysis for determination of contaminants of emerging concern in a sediment core collected from Beppu Bay, Japan2021

    • Author(s)
      Nishimuta Kou、Ueno Daisuke、Takahashi Shin、Kuwae Michinobu、Kadokami Kiwao、Miyawaki Takashi、Matsukami Hidenori、Kuramochi Hidetoshi、Higuchi Taiki、Koga Yuki、Matsumoto Hideaki、Ryuda Noriko、Miyamoto Hideki、Haraguchi Tomokazu、Sakai Shin-Ichi
    • Journal Title

      Environmental Pollution

      Volume: 272 Pages: 115587-115587

    • DOI

      10.1016/j.envpol.2020.115587

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Using the larvae of caddisfly as a biomonitor to assess the spatial distribution and effective half-life of radiocesium in riverine environments in Fukushima, Japan2021

    • Author(s)
      Matsuo, Y., Nakai, K., Tatsuta, N., Inanami, O., Yamamoto, K., Mizukawa, H., Nagasaka, H., Mizutani, F., Chisaki, Y., Aiba, T., Ohba, T., Watanabe, I., Nabeshi, H., Higuchi, T., Koga, Y., Matsumoto, H., Nishimuta, K., Miyamoto, H., Haraguchi, T., Ryuda, N., Ueno, D.
    • Journal Title

      Physics Open

      Volume: 6 Pages: 100060-100060

    • DOI

      10.1016/j.physo.2021.100060

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of specific odour compounds from garlic cloves infected with the potato tuber nematode, Ditylenchus destructor, using gas chromatography-olfactometry2021

    • Author(s)
      Koga Yuki、Yoshiga Toyoshi、Shindo Jun-ichi、Aoyama Rie、Nishimuta Kou、Koyama Reon、Miyamoto Hideki、Haraguchi Tomokazu、Ryuda Noriko、Ueno Daisuke
    • Journal Title

      Nematology

      Volume: 24 Issue: 1 Pages: 55-63

    • DOI

      10.1163/15685411-bja10111

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Distribution and characteristics of antagonistic bacteria in grass manure and grass manure amended arable soil in the Aso region of Kumamoto, Japan2021

    • Author(s)
      龍田 典子、居石 優子、古賀 夕貴、坂本 唯乃、三谷 果穂、阿部 紘乃、上野 大介、染谷 孝
    • Journal Title

      Soil Microorganisms

      Volume: 75 Issue: 2 Pages: 70-78

    • DOI

      10.18946/jssm.75.2_70

    • NAID

      130008109012

    • ISSN
      0912-2184, 2189-6518
    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2021-07-13   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi