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Regenerative medicine and Rehabilitation after peripheral nerve injury

Research Project

Project/Area Number 21K19709
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
Research InstitutionKyoto University

Principal Investigator

Kuroki Hiroshi  京都大学, 医学研究科, 教授 (20170110)

Co-Investigator(Kenkyū-buntansha) 谷間 桃子 (長井)  京都大学, 医学研究科, 助教 (50755676)
伊藤 明良  京都大学, 医学研究科, 講師 (50762134)
Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2021: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsリハビリテーション / 再生医療 / 末梢神経損傷 / 理学療法 / 超音波療法 / 非侵襲的磁気刺激 / 末梢神経 / 神経導管 / 緒音波療法
Outline of Research at the Start

末梢神経欠損部位に培養細胞を移植する再生医療ではリハビリテーションとの融合が重要である。本研究では、細胞で神経導管を作成し、これをラットに作成した坐骨神経欠損部に移植する。移植直前には神経導管の環境を制御するニッチ・リハビリテーションを、移植直後には神経導管の生着、順応、機能分化を促すセルラー・リハビリテーションを行い、ニッチからセルラーまで一体的、連続的に行う末梢神経欠損後の効果的なリハビリテーション・プロトコルを動物実験で検証する。リハビリテーション介入法は超音波刺激を用い、回復の比較には①組織学的評価、②遺伝子発現解析・免疫組織化学的評価、および③動物の3次元動作解析を用いる。

Outline of Final Research Achievements

In order to elucidate the mechanism of reinnervation after peripheral nerve injury by using ultrasound treatment and to verify the ability to suppress cell death of dorsal root ganglion (DRG) neurons by applying non-invasive magnetic stimulation, this project was conducted.
The results obtained indicate that (1) ultrasound treatment up to 1 week after injury is important to promote reinnervation and to reduce proinflammatory macrophages; and (2) non-invasive magnetic stimulation up to 1 week after injury may suppress DRG neuron cell death.

Academic Significance and Societal Importance of the Research Achievements

本課題の研究成果は、末梢神経損傷に対するリハビリテーションの臨床において、超音波治療による神経再生促進の可能性を示したこと、および非侵襲的磁気刺激を腰髄後根神経節部位に行うことで、後根神経節ニューロンの細胞死を抑制できる可能性を提示したことにあり、この領域の発展に貢献していると考える。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (23 results)

All 2024 2023 2022 2021

All Journal Article (9 results) (of which Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (14 results) (of which Int'l Joint Research: 6 results,  Invited: 1 results)

  • [Journal Article] Ultrasound therapy for a week promotes regeneration and reduces pro-inflammatory macrophages in a rat sciatic nerve autograft model.2023

    • Author(s)
      Kawai H, Ito A, Kawaguchi A, Nagai-Tanima M, Nakahara R, Xu S, Kuroki H.
    • Journal Title

      Sci Rep.

      Volume: 13 Issue: 1 Pages: 11494-11494

    • DOI

      10.1038/s41598-023-38630-8

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a novel model for intraarticular adhesion in rat knee joint.2023

    • Author(s)
      Nakahara R, Ito A, Nakahata A, Nagai-Tanima M, Kawai H, Uchiyama K, Nishitani K, Wang T, Aoyama T, Kuroki H.
    • Journal Title

      PLoS One

      Volume: 18 Issue: 9 Pages: e0292000-e0292000

    • DOI

      10.1371/journal.pone.0292000

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of Molecular Changes and Features in Rat Knee Osteoarthritis Cartilage: Progress From Cellular Changes to Structural Damage.2023

    • Author(s)
      Zhao Z, Ito A, Kuroki H, Aoyama T.
    • Journal Title

      Cartilage

      Volume: - Issue: 2 Pages: 232-249

    • DOI

      10.1177/19476035231213174

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expanding the targets of therapeutic electrophysical stimulation - For the advancement of peripheral nerve regenerative rehabilitation.2023

    • Author(s)
      Xu S, Ito A.
    • Journal Title

      Curr Opin Biomed Eng.

      Volume: 28 Pages: 100515-100515

    • DOI

      10.1016/j.cobme.2023.100515

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comprehensive Understanding of Inactivity-Induced Gait Alteration in Rodents2022

    • Author(s)
      Tajino Junichi、Aoyama Tomoki、Kuroki Hiroshi、Ito Akira
    • Journal Title

      Journal of Visualized Experiments

      Volume: 185 Issue: 185

    • DOI

      10.3791/63865

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultrasound Therapy of Injury Site Modulates Gene and Protein Expressions in the Dorsal Root Ganglion in a Sciatic Nerve Crush Injury Rat Model2022

    • Author(s)
      Xu Shixuan、Ito Akira、Wang Tianshu、Kawai Hideki、Aoyama Tomoki、Kuroki Hiroshi
    • Journal Title

      Ultrasound in Medicine & Biology

      Volume: 48 Issue: 12 Pages: 2502-2511

    • DOI

      10.1016/j.ultrasmedbio.2022.07.015

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultrasound Stimulation Inhibits Morphological Degeneration of Motor Endplates in the Denervated Skeletal Muscle of Rats2022

    • Author(s)
      Ito Akira、Araya Yuki、Kawai Hideki、Kuroki Hiroshi
    • Journal Title

      Neuroscience Insights

      Volume: 17

    • DOI

      10.1177/26331055221138508

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] One session of 20 N cyclic compression induces chronic knee osteoarthritis in rats: A long-term study2022

    • Author(s)
      Zhao Zixi、Ito Akira、Nakahata Akihiro、Ji Xiang、Tai Chia、Saito Motoo、Nishitani Kohei、Aoyama Tomoki、Kuroki Hiroshi
    • Journal Title

      Osteoarthritis and Cartilage Open

      Volume: 4 Issue: 4 Pages: 100325-100325

    • DOI

      10.1016/j.ocarto.2022.100325

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Regenerative Rehabilitation2021

    • Author(s)
      青山 朋樹, 池口 良輔, 伊藤 明良, 谷間 桃子, 南角 学, 田辺 直也, 小笹 寧子, 松田 秀一
    • Journal Title

      The Japanese Journal of Rehabilitation Medicine

      Volume: 58 Issue: 10 Pages: 1149-1155

    • DOI

      10.2490/jjrmc.58.1149

    • NAID

      130008121573

    • ISSN
      1881-3526, 1881-8560
    • Year and Date
      2021-10-18
    • Related Report
      2021 Research-status Report
  • [Presentation] Magnetic Stimulation Prevents Dorsal Root Ganglion Neuron Death and Enhances Nerve Regeneration in a Sciatic Nerve Injury Rat Model.2024

    • Author(s)
      Shixuan Xu, Akira Ito, Zixi Zhao, Ryo Nakahara, Chia Tai, Fumika Miyamoto, Hiroshi Kuroki, Tomoki Aoyama.
    • Organizer
      Tenth Annual International Symposium on Regenerative Rehabilitation
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 坐骨神経損傷モデルラットに対する磁気刺激治療がニューロン細胞死に与える影響の検討.2024

    • Author(s)
      徐仕軒,伊藤明良,戴嘉,中原崚,趙梓汐,宮本史佳,黒木裕士,青山朋樹.
    • Organizer
      日本物理療法合同学術大会2024
    • Related Report
      2023 Annual Research Report
  • [Presentation] Ultrasound therapy for a week after injury accelerates the regeneration in a rat sciatic nerve autograft model2022

    • Author(s)
      Hideki Kawai, Akira Ito, Momoko Nagai-Tanima, Ryo Nakahara, Hiroshi Kuroki
    • Organizer
      Ninth Annual International Symposium on Regenerative Rehabilitation
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of gene expression after ultrasound therapy for intraarticular adhesion model in rats2022

    • Author(s)
      Ryo Nakahara, Akira Ito, Momoko Nagai-Tanima, Hideki Kawai, Zixi Zhao, Chia Tai, Hiroshi Kuroki
    • Organizer
      Ninth Annual International Symposium on Regenerative Rehabilitation
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Decelerating the process of articular cartilage degeneration in a non-invasive mechanical compression-induced rat model via intra-articular injections of allogeneic BMSCs2022

    • Author(s)
      Chia Tai, Akira Ito, Zixi Zhao, Ryo Nakahara, Hideki Kawai, Hiroshi Kuroki
    • Organizer
      Ninth Annual International Symposium on Regenerative Rehabilitation
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] The effect of ultrasound therapy on dorsal root ganglion in sciatic nerve crush injury rat model2022

    • Author(s)
      Shixuan Xu, Akira Ito, Hideki Kawai, Ryo Nakahara, Zixi Zhao, Chia Tai, Tomoki Aoyama, Hiroshi Kuroki
    • Organizer
      Ninth Annual International Symposium on Regenerative Rehabilitation
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] ラット膝関節の術後癒着に対する超音波照射強度と時間の影響の検証2022

    • Author(s)
      中原崚,伊藤明良,谷間桃子,河合秀紀,趙梓汐,徐仕軒,戴嘉,安孫子幸子,青山朋樹,黒木裕士
    • Organizer
      第27回日本基礎理学療法学会学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 坐骨神経挫滅損傷モデルラットに対する超音波治療が脊髄後根神経節に与える影響の検討2022

    • Author(s)
      徐仕軒,伊藤明良,河合秀紀,中原崚,趙梓汐,戴嘉,青山朋樹,黒木裕士
    • Organizer
      第27回日本基礎理学療法学会学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 超音波パルス刺激は脱神経筋におけるアセチルコリン受容体の形態変性を抑制する2022

    • Author(s)
      伊藤明良,河合秀紀,中原崚,徐仕軒,趙梓汐,戴嘉,黒木裕士
    • Organizer
      第27回日本基礎理学療法学会学術大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 再生リハビリテーションの現状と展望2021

    • Author(s)
      伊藤明良
    • Organizer
      第61回近畿理学療法学術大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 再生医学とリハビリテーション2021

    • Author(s)
      黒木 裕士
    • Organizer
      第26回日本基礎理学療法学会学術大会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 物理療法と再生リハビリテーション2021

    • Author(s)
      伊藤明良、河合秀紀、黒木裕士
    • Organizer
      第60回日本生体医工学会大会オーガナイズドセッションOS2-7-1
    • Related Report
      2021 Research-status Report
  • [Presentation] 末梢神経再生に最適な 超音波刺激方法の開発2021

    • Author(s)
      河合秀紀、伊藤明良、王天舒、黒木裕士
    • Organizer
      第60回日本生体医工学会大会オーガナイズドセッションOS2-7-1
    • Related Report
      2021 Research-status Report
  • [Presentation] OPTIMIZATION OF REHABILITATION FOR FURTHER PROGRESS IN PERIPHERAL NERVE REGENERATION2021

    • Author(s)
      Hideki Kawai
    • Organizer
      Rising Stars of Regenerative Rehabilitation Virtual Speaker Series 2021, The Alliance for Regenerative Rehabilitation Research and Training
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research

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Published: 2021-07-13   Modified: 2025-01-30  

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