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Elucidata the mechanism of force potentiation by countermovement based on the visualization of the sarcomere length

Research Project

Project/Area Number 21K19744
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 59:Sports sciences, physical education, health sciences, and related fields
Research InstitutionRitsumeikan University

Principal Investigator

Fukutani Atsuki  立命館大学, スポーツ健康科学部, 講師 (80722644)

Co-Investigator(Kenkyū-buntansha) 伊坂 忠夫  立命館大学, スポーツ健康科学部, 教授 (30247811)
Project Period (FY) 2021-07-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2022: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2021: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Keywordsミオシン / アクチン / タイチン / サルコメア / 骨格筋 / クロスブリッジ / 反動動作
Outline of Research at the Start

筋が発揮する力はサルコメア長に依存するため、サルコメア長を把握することは力発揮メカニズムを解明する上で必須である。これまでは、筋線維内の各サルコメアの長さは全て均一と考えて議論がなされてきた。しかしながら、実際にサルコメアの長さを可視化してみると、必ずしもサルコメアの長さは均一ではなく、筋を引き伸ばした時のサルコメアの伸長量も、サルコメア間で違いが見られる。そうなると、各サルコメアの長さ変化量は全てのサルコメアで一定という前提条件のもとに進めていた議論は再検討する必要がある。そこで本研究では、反動動作中に各サルコメアの長さを可視化することで、反動動作による力発揮能力増強の実態解明を目指す。

Outline of Final Research Achievements

The isometric force is increased after an eccentric contraction compared to the pure isometric force. This phenomenon is called residual force enhancement. Currently, the mechanism of this force potentiation is considered to be sarcomere length non-uniformity. However, this idea is an estimation based on the theory and not yet experimentally verified. Therefore, the purpose of this study was to examine whether this estimation is correct or not by visualizing the sarcomere length during contractions. As a result, although the force potentiation was confirmed as expected, the variation of sarcomere length was not different between conditions. This result indicates that the widely-used sarcomere length non-uniformity theory could not explain the force potentiation after an eccentric contraction and should be reconsidered.

Academic Significance and Societal Importance of the Research Achievements

これまで、サルコメア長の変化は均一と考え、「筋線維が10%短縮したということは、各サルコメアが均一に10%短縮した」とみなして、力学現象を説明してきた。しかしながら、本研究によって、サルコメア長の変化は均一ではなく、伸ばされて長くなるサルコメアもあれば、反対に短い状態を維持したままのサルコメアも存在することが明らかになった。サルコメアの長さが均一でないからこそ生じる現象も存在することから、各サルコメア長の実測が必要ということを提唱することは、身体運動の詳細な生成メカニズム解明に向けて当該分野の理解を一歩進めるという点で、学術的意義がある。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (5 results)

All 2023 2022

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (1 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Measurements of tendon length changes during stretch-shortening cycles in rat soleus2023

    • Author(s)
      Fukutani Atsuki、Hashizume Satoru、Isaka Tadao
    • Journal Title

      Scientific Reports

      Volume: 13 Issue: 1 Pages: 5381-5381

    • DOI

      10.1038/s41598-023-32370-5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Force loss induced by inhibiting cross-bridge cycling is mitigated in eccentric contraction2022

    • Author(s)
      Fukutani Atsuki、Kunimatsu Shiho、Isaka Tadao
    • Journal Title

      Journal of Experimental Biology

      Volume: 225 Issue: 21

    • DOI

      10.1242/jeb.244962

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Influence of caffeine on the maximal isometric and concentric force produced by skinned fibers2022

    • Author(s)
      Fukutani Atsuki、Kunimatsu Shiho、Isaka Tadao
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 7980-7980

    • DOI

      10.1038/s41598-022-12222-4

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Residual force enhancement is attenuated for quick stretch conditions2022

    • Author(s)
      Fukutani Atsuki、Herzog Walter
    • Journal Title

      Journal of Biomechanics

      Volume: 136 Pages: 111076-111076

    • DOI

      10.1016/j.jbiomech.2022.111076

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Sarcomere length variations in the pure isometric and the enhanced isometric conditions in the skinned single fiber preparations2022

    • Author(s)
      Fukutani Atsuki、Kunimatsu Shiho、Isaka Tadao
    • Organizer
      9th World Congress on Biomechanics
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research

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Published: 2021-07-13   Modified: 2024-01-30  

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