| Project/Area Number |
21K20526
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0403:Biomedical engineering and related fields
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
Le Hue Thi 国立研究開発法人国立循環器病研究センター, 研究所, リサーチフェロー (80906280)
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| Project Period (FY) |
2021-08-30 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | injectable hydrogel / myocardial infarction / sorbitol response / adverse remodeling / MSCs / material distribution / material retention / cardiac function / viscosity / wide-distribution / long-retention / sorbitol responsive / mesenchymal stem cell / cell viability / ventricular regeneration |
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| Outline of Research at the Start |
This study aims to confirm a new concept of injectable hydrogel with elongates mesenchymal stem cells (MSCs) viability in the rat heart. This hydrogel enables sol-gel transition automatically via simple diffusion of glucose molecules to the host body without exogenous stimuli such as thermal, UV light, irradiation used for gelation of reported injectable hydrogels. Using an animal model of myocardial infarction, we next to investigate the effect of this MSCs-hydrogel complex on cardiac function, and ventricular regeneration.
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| Outline of Final Research Achievements |
A new sugar-responsive injectable hydrogel was developed for the treatment of myocardial infarction by combining it with or without mesenchymal stem cells (MSCs). We achieved the proof-of-concept in which the material solution included poly (3-acrylamidophenylboronic acid-co-acrylamide) (BAAm), poly(vinyl alcohol) (PVA), and sorbitol can become an in-situ gel after injection into the tissue via simple diffusion of the sorbitol to surrounding tissue. It has been found that those materials have no cytotoxicity with several cell types involving MSCs, smooth muscle cells, and fibroblast cells. Using a myocardial infarction model, we demonstrated that intramyocardial injection of this material prevents adverse cardiac remodeling, resulting in preserved cardiac function. The current data was published in a peer-reviewed journal and presented at six domestic and international scientific conferences.
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| Academic Significance and Societal Importance of the Research Achievements |
The findings of this study suggest that sorbitol-responsive injectable hydrogel may be a novel therapeutic approach for myocardial infarction. Moreover, this study proposes a material concept that may surmount the low retention of cells and drugs to the target tissue.
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