| Project/Area Number |
21K20587
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
0602:Agricultural and environmental biology and related fields
|
|---|
| Research Institution | National Agriculture and Food Research Organization |
|---|
Principal Investigator |
INUI Tomohiro 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, 契約研究員 (50911020)
|
|---|
| Project Period (FY) |
2021-08-30 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 幼若ホルモン / 変態 / 脱皮 / アラタ体 / 転写因子 / 生合成制御 / 生合成酵素 |
|---|
| Outline of Research at the Start |
幼若ホルモン（JH）は昆虫に特有な変態抑制作用を持つ重要なホルモンであるが、その生合成制御メカニズムには未解明な点が多い。本研究では、JH生合成器官であるアラタ体で特異的に発現し、終齢幼虫期のみでJH生合成を停止させて幼虫を次のステージへと変態可能にする未同定因子の単離と機能解析を行う。
|
| Outline of Final Research Achievements |
In this study, we searched for genes that specifically repress the expression of JHAMT, a key juvenile hormone (JH) biosynthetic enzyme, (JHAMT Repressor, JHAMT-Rep) in the corpora allata (CA) in a final instar-specific manner. We comprehensively searched for genes whose expression is upregulated specifically in the last instar larvae by RNA-seq using total RNA extracted from CA, and created an RNAi library based on the transcriptomic data. The RNAi screening was performed using Red flour beetle, Tribolium castaneum, and the candidate genes for JHAMT-Rep were identified. RNAi knockdown of JHAMT-Rep candidate gene resulted in supernumerary larvae in T. castaneum. This result suggested that JH concentration in the body did not decrease in RNAi treated animals, and thus they could not metamorphose.
|
| Academic Significance and Societal Importance of the Research Achievements |
JHの変態抑制作用は昆虫に普遍的かつ特徴的な生理作用であり、昆虫の繁栄の要因の一つであることから、学術的な興味はもとより創農薬の有望なターゲットとして注目されており、その生合成やシグナル経路の解明が急務である。本研究の成果は、前述のJHAMT-Rep候補遺伝子の同定と機能解析から、JHの生合成制御機構の解明に貢献した。
|
