| Project/Area Number |
21K20633
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Multi-year Fund |
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| Review Section |
0701:Biology at molecular to cellular levels, and related fields
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| Research Institution | Kanazawa University |
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Principal Investigator |
Hall Damien 金沢大学, ナノ生命科学研究所, 特任助教 (50447950)
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| Project Period (FY) |
2021-08-30 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | virus / cell membrane / virus entry / colloidal adsorption / computer modelling / virus-cell entry / biophysical modelling / quantitative biology / biophysics of disease |
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| Outline of Research at the Start |
This study will involve both experimental development of in vitro model systems of virus-like-particles (VLP) binding to a membrane-like-surface (MLS), and computational development of simulation models of VLP/MLS
interactions leading to the identification of new drug targets.
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| Outline of Final Research Achievements |
On the computational side two computer models were developed, and on the experimental side a VLP/MLS assay was established based on optical microscope visualization of metal colloid adsorption to a chemically coated gold glass surface. These results are currently being written up. The funds from this start-up project were acknowledged in the following published or submitted manuscript manuscripts using the following appropriation, ‘This work was supported, in part, by KAKENHI Start-Up grant 21K20633 awarded to D.H.’ (i) Hall, D. and Foster, A.S. (2022) Practical considerations for feature assignment in high-speed AFM of live cell membranes. Biophysics and Physicobiology, 19, p.e190016. https://doi.org/10.2142/biophysico.bppb-v19.0016 (ii) Hall, D. (2023) MIL Cell: A tool for multi-scale simulation of yeast replication and prion transmission. BioRxiv, pp.2023-03. https://doi.org/10.1101/2023.03.21.533288
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| Academic Significance and Societal Importance of the Research Achievements |
This work potentially serves society by, 1. It provides greater knowledge about the colloidal forces experienced by a virus at the cell membrane. 2. It brings ideas ideas from two scientific disciplines together (virology and colloidal science). 3. It highlights new avenues for pharmacotherapy.
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