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Development of rapidly constructible bone regeneration materials to treat large bone defect

Research Project

Project/Area Number 21K20978
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0906:Surgery related to the biological and sensory functions and related fields
Research InstitutionKitasato University

Principal Investigator

Shoji Shintaro  北里大学, 医学部, 助教 (60728202)

Project Period (FY) 2021-08-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords局所硬化ゲル / 多孔質材料 / 骨欠損治療 / 塩基性線維芽細胞増殖因子 / 骨形成促進 / BMP-2 / 成長因子導入 / 細胞導入 / 硬化ゲル
Outline of Research at the Start

広範囲骨欠損は治療に難渋する。本研究ではゲル剤の特性を利用した多孔質材料への迅速な細胞、成長因子導入法の確立と新規材料を用いた骨欠損治療法の開発を目指す。多孔質人工骨への迅速な細胞、成長因子導入法による難治性骨折治療法の確立を目指し、1. 多孔質材料への細胞、成長因子導入に適した硬化ゲルの開発、2. マウス、ラット骨欠損モデルを用いたシーズの有用性の検討、3. 実用化に向けたヒト細胞を用いた検証を行う。

Outline of Final Research Achievements

We examined the effectiveness of in situ-formed gels (IFGs) consisting of hyaluronan (HyA)-tyramine (TA), horseradish peroxidase and hydrogen peroxide enhance the bone formation ability of hydroxyapatite (Hap)/BMP-2 composites for facilitating osteogenesis in refractory fracture model mice. After establishing the refractory fracture model, animals were either treated at the site of fracture with IFGs-HyA with Hap harboring BMP-2 (IFGs-HyA/Hap/BMP-2). We determined the extent of bone formation at the fracture site according to findings on micro-computed tomography and histological experiments. Animals treated with IFGs-HyA/Hap/BMP-2 demonstrated significantly greater bone volume, bone mineral content and bone union than those treated with vehicle or IFG-HyA/Hap alone. IFGs-HyA/Hap/BMP-2 could be an effective treatment option for refractory fractures.

Academic Significance and Societal Importance of the Research Achievements

局所硬化ゲルの多孔質材料への成長因子導入法を確立し、その有用性を示した。本方法は骨欠損治療のみならず、再生医療全般に応用できるものと考えられる。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (2 results)

All 2021

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Acceleration of bone formation using in situ-formed hyaluronan-hydrogel containing bone morphogenetic protein-2 in a mouse critical size bone defect model2021

    • Author(s)
      Shoji S, Uchida K, Tazawa R, Saito W, Kuroda A, Sekiguchi H, Ishii D, Inoue S, Inoue G, Takaso M
    • Journal Title

      Bio-Medical Materials and Engineering

      Volume: - Issue: 4 Pages: 207-215

    • DOI

      10.3233/bme-201172

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] BMP-2含有局所硬化ヒアルロン酸ゲルを用いた広範囲骨欠損修復法の有用性2021

    • Author(s)
      庄司真太郎、内田健太郎、田澤諒、関口裕之、井上 玄、齋藤亘、宮城正行、黒田晃義、高相晶士.
    • Organizer
      第36回日本整形外科学会基礎学術集会
    • Related Report
      2021 Research-status Report

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Published: 2021-10-22   Modified: 2024-01-30  

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