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Creation of genome manipulation technology using super restriction enzyme

Research Project

Project/Area Number 22000007
Research Category

Grant-in-Aid for Specially Promoted Research

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Chemistry
Research InstitutionUniversity of Tsukuba (2011-2015)
The University of Tokyo (2010)

Principal Investigator

KOMIYAMA Makoto  筑波大学, 生命領域学際研究センター, 教授 (50133096)

Co-Investigator(Kenkyū-buntansha) SUMAOKA Jun  筑波大学, 生命領域学際研究センター, 講師 (10280934)
XU Yan  宮崎大学, 医学部, 教授 (40506763)
ITO Yasuhiro  東京大学, 分子細胞生物学研究所, 助教 (50508108)
HIGUCHI Maiko  東京大学, 分子細胞生物学研究所, 助教 (30420235)
Co-Investigator(Renkei-kenkyūsha) SHIGI Narumi  筑波大学, 生命領域学際研究センター, 研究員 (00396780)
AIBA Yuichiro  筑波大学, 生命領域学際研究センター, 研究員 (10581085)
Project Period (FY) 2010 – 2015
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥520,520,000 (Direct Cost: ¥400,400,000、Indirect Cost: ¥120,120,000)
Fiscal Year 2014: ¥76,830,000 (Direct Cost: ¥59,100,000、Indirect Cost: ¥17,730,000)
Fiscal Year 2013: ¥76,440,000 (Direct Cost: ¥58,800,000、Indirect Cost: ¥17,640,000)
Fiscal Year 2012: ¥77,480,000 (Direct Cost: ¥59,600,000、Indirect Cost: ¥17,880,000)
Fiscal Year 2011: ¥151,190,000 (Direct Cost: ¥116,300,000、Indirect Cost: ¥34,890,000)
Fiscal Year 2010: ¥138,580,000 (Direct Cost: ¥106,600,000、Indirect Cost: ¥31,980,000)
KeywordsDNA / 制限酵素 / ペプチド核酸 / 遺伝子組換え / セリウム / ゲノム / 遺伝子 / バイオテクノロジー / 核酸 / 遺伝子操作 / ゲノム編集 / 相同組替え / ペプチド / 人工酵素 / 相同組換え / バイテクノロジー
Outline of Final Research Achievements

Man-mode tool (super artificial restriction enzyme), developed by our group, is highly site-selective and cuts huge human genome at one target site. In this research, this cutter was used to obtain desired fragments from human genome. Telomeres and other fragments were clipped from human genome with high selectivity. It was found that the telomere length is different from a chromosome to another even in the same cells. This unexpected finding has never been obtained by previous methods, showing superb roles of the present chemical cutter for molecular biology. Even in alive human cells, the super artificial restriction enzyme site-selectively cut the genome, inducing desired homologous recombination. Furthermore, the function of the cutters were improved by various chemical modifications. Attachment of nuclear localization signal peptide to PNA was especially effective and allowed the invasion of the PNA into the genome to proceed spontaneously under physiological conditions.

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(10 results)
  • 2016 Research Progress Assessment (Verification Result) ( PDF )
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (115 results)

All 2016 2015 2014 2013 2012 2011 2010 Other

All Journal Article (41 results) (of which Peer Reviewed: 40 results,  Acknowledgement Compliant: 2 results,  Open Access: 2 results) Presentation (66 results) (of which Int'l Joint Research: 2 results,  Invited: 7 results) Book (4 results) Remarks (4 results)

  • [Journal Article] Fabrication of DNA/RNA hybrids through sequence-specific scission of both strands by pcPNA-SI nuclease combination2016

    • Author(s)
      K. Futai, J. Sumaoka, M. Komiyama
    • Journal Title

      Nucleosides Nucleotides Nucleic Acids

      Volume: 35 Issue: 5 Pages: 233-244

    • DOI

      10.1080/15257770.2015.1131294

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Affinity-isolation of desired restriction fragment from human genome using double-duplex invasion of biotin-bound pseudo-complementary PNA.2015

    • Author(s)
      N. Shigi, A. Rajendran, X. Wang, H. Kunifuda, J. Sumaoka, M. Komiyama
    • Journal Title

      Chem. Lett.

      Volume: 44 Issue: 11 Pages: 1569-1571

    • DOI

      10.1246/cl.150682

    • NAID

      130005108088

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clipping of Telomere from Human Chromosomes using Chemistry-based Artificial Restriction DNA Cutter2015

    • Author(s)
      Takumi Ishizuka, Yan Xu, Makoto Komiyama
    • Journal Title

      Curr. Protoc. Nucleic Acid Chem.

      Volume: in press Issue: 1

    • DOI

      10.1002/0471142700.nc0613s61

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Promotion of double-duplex invasion of peptide nucleic acid through conjugation with nuclear localization signal peptide2015

    • Author(s)
      Y. Aiba, Y. Honda, M. Komiyama
    • Journal Title

      Chem. Eur. J.

      Volume: 21 Issue: 10 Pages: 4021-4026

    • DOI

      10.1002/chem.201406085

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote its in vivo and in vitro applications2015

    • Author(s)
      M. Komiyama
    • Journal Title

      Artificial DNA : PNA & XNA

      Volume: 5 Issue: 3 Pages: 1-13

    • DOI

      10.1080/1949095x.2015.1112457

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clipping of Telomere from Human Chromosomes using Chemistry-based Artificial Restriction DNA Cutter2015

    • Author(s)
      T. Ishizuka, Y. Xu, and M. Komiyama
    • Journal Title

      Curr. Protoc. Nucleic Acid Chem

      Volume: 61

    • Description
      in press
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Thiazole orange-conjugated peptide nucleic acid for fluorescent detection of specific DNA sequences and site-selective photodamage2014

    • Author(s)
      Tanaka, M, et al.
    • Journal Title

      RSC advances

      Volume: 4 Issue: 108 Pages: 63533-63538

    • DOI

      10.1039/c4ra13780a

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Clipping of predetermined fragments from human genome by Slnuclease-PNA combinations2014

    • Author(s)
      X. Li, S. Muneoka, N. Shigi, J. Sumaoka, and M. Komiyama
    • Journal Title

      Chem. Commun.

      Volume: 50 Issue: 63 Pages: 8674-8676

    • DOI

      10.1039/c4cc01420k

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Crowding Facilitates Double-duplex Invasion of Pseudo-complementary Peptide Nucleic Acid in High Salt Medium2014

    • Author(s)
      J. Sumaoka and M. Komiyama
    • Journal Title

      Chem. Lett.

      Volume: 43 Issue: 10 Pages: 1581-1583

    • DOI

      10.1246/cl.140620

    • NAID

      130004693816

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Finding of a human telomere DNA-RNA hybrid G-quadruplex formed by human telomeric 6-mer RNA and 16-mer DNA using click chemistry : Aprotective structure for telomere end2014

    • Author(s)
      Y. Xu, Y. Suzuki, T. Ishizuka, C.-D. Xiao, X. Liu, T. Hayashi, and M. Komiyama
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 22 Issue: 16 Pages: 4419-4421

    • DOI

      10.1016/j.bmc.2014.05.053

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Orthogonal enzyme arrays on a DNA origami scaffold bearing size-tunable wells2014

    • Author(s)
      T. Yamazaki, J.G. Heddle, A. Kuzuya, and M. Komiyama
    • Journal Title

      Nanoscale

      Volume: 6 Issue: 15 Pages: 9122-9126

    • DOI

      10.1039/c4nr01598c

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Site-Selective Scission of Human Genome Using PNA-Based Artificial Restriction DNA Cutter2014

    • Author(s)
      K. Ito and M. Komiyama
    • Journal Title

      Methods Mol. Biol.

      Volume: 1050 Pages: 111-120

    • DOI

      10.1007/978-1-62703-553-8_9

    • ISBN
      9781627035521, 9781627035538
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeting Human Telomeric Higher-Order DNA : Dimeric G-quadruplex Units Serve as Preferred Binding Site2013

    • Author(s)
      C. Zhao, L. Wu, J. Ren, Y. Xu and X. Qu
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Issue: 50 Pages: 18786-18789

    • DOI

      10.1021/ja410723r

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conjugation of peptide nucleic acid with pyrrole-imidazole polyamide to specifically recognize and cleave DNA2013

    • Author(s)
      W. Kameshima, T. Ishizuka, M. Minoshima, H. Sugiyama, Y. Xu, and M. Komiyama
    • Journal Title

      Angew. Chem. Int. Ed. Engl.

      Volume: 52 Issue: 51 Pages: 13681-13684

    • DOI

      10.1002/anie.201305489

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct light-up of cAMP derivatives in living cells by click reactions2013

    • Author(s)
      K. Ito, H. Liu, M. Komiyama, T. Hayashi, and Y. Xu
    • Journal Title

      Molecules

      Volume: 18 Issue: 10 Pages: 12909-12915

    • DOI

      10.3390/molecules181012909

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-selective scission of double-stranded DNA by combining a triplex-forming bis-PNA and Ce (IV)/EDTA2013

    • Author(s)
      Y. Aiba, K. Yasuda. and M. Komiyama
    • Journal Title

      Chemistry Letters

      Volume: 42 Issue: 10 Pages: 1300-1302

    • DOI

      10.1246/cl.130650

    • NAID

      10031201345

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An artificial restriction DNA cutter for site-selective gene insertion in human cells.2013

    • Author(s)
      Kenichiro Ito, Narumi Shigi, Makoto Komiyama
    • Journal Title

      Chemical Communications

      Volume: 49 Issue: 60 Pages: 6764-6766

    • DOI

      10.1039/c3cc43261k

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cut-and-paste of DNA using artificial restriction DNA cutter2013

    • Author(s)
      M. Komiyama
    • Journal Title

      Int. J. Mol. Sci.

      Volume: 14 Pages: 3343-3357

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNAを切る唯一の触媒―セリウムとの幸運な出会い―2013

    • Author(s)
      小宮山 眞
    • Journal Title

      別冊化学"ケミストを魅了した元素と周期律表"

      Volume: 5月号 Pages: 12-12

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] PNA-NLS conjugates as single-molecular activators of target sites in double-stranded DNA for site-selective scission2013

    • Author(s)
      Y. Aiba, Y. Hamano, W. Kameshima, Y. Araki, T. Wada, A. Accetta, S. Sforza, R. Corradini, R. Marchelli, and M. Komiyama
    • Journal Title

      Org. Biomol. Chem.

      Volume: 11 Issue: 32 Pages: 5233-5238

    • DOI

      10.1039/c3ob40947c

    • NAID

      120007130454

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evidence for G-quadruplex DNA in human cells2013

    • Author(s)
      Y. Xu and M. Komiyama
    • Journal Title

      Chembiochem.

      Volume: 14 Issue: 8 Pages: 927-928

    • DOI

      10.1002/cbic.201300157

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Chemistry-Based Method to Detect Individual Telomere Lengths at a Single Chromosome Terminus2013

    • Author(s)
      T. Ishizuka, Y. Xu, and M. Komiyama
    • Journal Title

      J. Am. Chem. Soc.

      Volume: 135 Issue: 1 Pages: 14-17

    • DOI

      10.1021/ja308481c

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oligonucleotide Models of Telomeric DNA and RNA Form a Hybrid G-quadruplex Structure as a Potential Component of Telomeres2012

    • Author(s)
      Yan Xu, Takumi Ishizuka, Jie Yang, Kenichiro Ito, Hitoshi Katada, Makoto Komiyama, Tetsuya Hayashi
    • Journal Title

      J. Bio. Chem.

      Volume: Vol. 287 Issue: 50 Pages: 41787-41796

    • DOI

      10.1074/jbc.m112.342030

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structure, function and targeting of human telomere RNA2012

    • Author(s)
      Y. Xu and M. Komiyama
    • Journal Title

      Methods

      Volume: 57 Issue: 1 Pages: 100-105

    • DOI

      10.1016/j.ymeth.2012.02.015

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 高等生物を対象とした新しいバイオテクノロジー  -スーパー制限酵素(化学合成ツール)の活用―2012

    • Author(s)
      小宮山 眞
    • Journal Title

      未来材料

      Volume: 12 Pages: 64-66

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Introduction of multiphosphonate ligand to peptide nucleic acid for metal ion conjugation.2012

    • Author(s)
      Y. Aiba
    • Journal Title

      Artificial DNA: PNA & XNA

      Volume: 3 Issue: 2 Pages: 73-79

    • DOI

      10.4161/adna.20727

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical and biological approaches to improve the efficiency of homologous recombination in human cells mediated by artificial restriction DNA cutter. H.2012

    • Author(s)
      H. Katada
    • Journal Title

      Nucleic Acids Res.

      Volume: 40 Issue: 11 Pages: e81-e81

    • DOI

      10.1093/nar/gks185

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clear-cut Observation of PNA Invasion using Nanomechanical DNA Origami Devices.2012

    • Author(s)
      T. Yamazaki
    • Journal Title

      Chem. Commun.

      Volume: 48 Issue: 92 Pages: 11361-11363

    • DOI

      10.1039/c2cc36358e

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and applications of artificial restriction DNA cutters for site-selective scission of genomes.2012

    • Author(s)
      M. Komiyama
    • Journal Title

      Bull. Chem. Soc. Jpn.

      Volume: 85 Issue: 5 Pages: 533-544

    • DOI

      10.1246/bcsj.20110318

    • NAID

      130004153074

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enzyme Treatment-Free and Ligation-Independent Cloning Using Caged Primers in Polymerase Chain Reaction.2012

    • Author(s)
      A. Kuzuya
    • Journal Title

      Molecules

      Volume: 17 Issue: 1 Pages: 328-340

    • DOI

      10.3390/molecules17010328

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intracellular localization of PNA in human cells upon its introduction by electroporation.2012

    • Author(s)
      N. Shigi
    • Journal Title

      Nat. Product Commun.

      Volume: 7 Pages: 349-352

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial site-selective DNA cutters to manipulate single-stranded DNA.2012

    • Author(s)
      Y. Aiba
    • Journal Title

      Polym. J.

      Volume: 52 Issue: 9 Pages: 413-435

    • DOI

      10.1038/pj.2012.37

    • NAID

      10031008879

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] G-rich Sequence-specific Recognition and Scission of Human Genome by PNA/DNA Hybrid G-quadruplex Formation2012

    • Author(s)
      Takumi Ishizuka, Makoto Komiyama, Yan Xu
    • Journal Title

      Angew. Chem., Int. Ed.

      Volume: Vol. 51 Issue: 29 Pages: 7198-7202

    • DOI

      10.1002/anie.201201176

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial DNA cutters for DNA manipulation and genome engineering.2011

    • Author(s)
      Y. Aiba
    • Journal Title

      Chem. Soc. Rev.

      Volume: 40 Issue: 12 Pages: 5657-5668

    • DOI

      10.1039/c1cs15039a

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artificial restriction DNA cutters to promote homologous recombination in human cells2011

    • Author(s)
      H.Katada, M.Komiyama
    • Journal Title

      Current Gene Therapy

      Volume: 11 Pages: 38-45

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] スーパー制限酵素を用いた遺伝子改変2011

    • Author(s)
      須磨岡淳
    • Journal Title

      高分子

      Volume: 2 Pages: 204-208

    • NAID

      10027971920

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Nanomechanical DNA Origami 'Single-Molecule Beacons' Directly Imaged by Atomic Force Microscopy2011

    • Author(s)
      A.Kuzuya, M.Komiyama
    • Journal Title

      Nature Commun.

      Volume: 2 Pages: 449-449

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photo-Switching of Site-Selective RNA Scission by Sequential Icorporation of Azobenzene and Acridine Residues in a DNA Oligomer2011

    • Author(s)
      A.Kuzuya, M.Komiyama
    • Journal Title

      J.Nucl.Acids

      Volume: 2011 Pages: 162452-162452

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Manipulation of single-stranded DNA using artificial site-selective DNA cutter composed of Ce(IV)/EDTA and phosphonate-oligonucleotide conjugates2010

    • Author(s)
      Y.Aiba, M.Komiyama
    • Journal Title

      Chem.Asian J.

      Volume: 6 Pages: 2407-2411

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Telomeric repeat-containing RNA structure in living cells2010

    • Author(s)
      Y.Xu
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107 Pages: 4579-14584

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A U-tetrad stabilizes human telomeric RNA G-quadruplex structure2010

    • Author(s)
      Y.Xu
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 132 Pages: 7231-7233

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] リボヌクレアーゼAによる単一鎖ペプチド核酸の二本鎖DNAへのインベージョン促進2016

    • Author(s)
      田仲真紀子・鴫 成実・小宮山 眞
    • Organizer
      日本化学会96春季年会
    • Place of Presentation
      同志社大学京田辺キャンパス(京都府・京田辺市)
    • Year and Date
      2016-03-25
    • Related Report
      2015 Annual Research Report
  • [Presentation] Chemical Tools to Isolate a Desired Fragment from Human Genome2015

    • Author(s)
      Makoto Komiyama
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2015-12-20
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Covalent and Noncovalent Approaches to Promote PNA Invasion for In Vivo Applications2015

    • Author(s)
      Makoto Komiyama
    • Organizer
      The 2015 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii (USA)
    • Year and Date
      2015-12-17
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] RNase A添加によるDNAへのペプチド核酸のインベージョン促進2015

    • Author(s)
      田仲真紀子・鴫 成実・小宮山 眞
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] ビオチン化pcPNAプローブによるヒトゲノムの特定領域断片の濃縮法の開発2015

    • Author(s)
      鴫(日野) 成実, アリワラガン ラジェンドラン, 王 曉輝, 国府田 寛子, 須磨岡 淳, 小宮山 眞
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県・神戸市)
    • Year and Date
      2015-12-04
    • Related Report
      2015 Annual Research Report
  • [Presentation] シクロデキストリン科学の変遷2015

    • Author(s)
      小宮山眞
    • Organizer
      第8回アジアンシクロデキストリンカンファレンス/第32回シクロデキストリンシンポジウム合同会議
    • Place of Presentation
      くまもと県民交流館パレア(熊本県熊本市)
    • Year and Date
      2015-05-15
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] チアゾールオレンジ修飾ペプチド核酸による二本鎖DNA中での特定配列の蛍光検出と位置選択的光損傷2015

    • Author(s)
      田仲真紀子、鴫成美、須磨岡淳、小宮山眞
    • Organizer
      第95回日本化学会春季年会
    • Place of Presentation
      日本大学船橋キャンパス(千葉県船橋市)
    • Year and Date
      2015-03-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] Site-specific scission of human genome for efficient isolation of its specific region2014

    • Author(s)
      A. Rajendran, N. Shigi, X. Li, J. Sumaoka, M. Komiyama
    • Organizer
      The 41st International symposium on nucleic acids chemistry
    • Place of Presentation
      Kitakyushu international conference center(福岡県北九州市)
    • Year and Date
      2014-11-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] DNA四重鎖構造を解析する機能性プローブの開発2014

    • Author(s)
      石塚 匠, 徐 岩
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス(愛知県名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 19F-labeled telomere RNA G-quadruplex structure2014

    • Author(s)
      BAO Hongliang, ISHIZUKA Takumi, XU Yan
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス(愛知県名古屋市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Sequence specific cleavage of DNA/RNA hybrids2013

    • Author(s)
      Kazuki Futai, Makoto Komiyama
    • Organizer
      ISNAC2013第40回国際核酸化学シンポジウム
    • Place of Presentation
      神奈川大学(神奈川)
    • Year and Date
      2013-11-13
    • Related Report
      2013 Annual Research Report
  • [Presentation] 分子クラウディング効果によるPNAインベージョンの促進2013

    • Author(s)
      須磨岡淳, 小宮山眞
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋大学東山キャンバス(愛知県)
    • Year and Date
      2013-09-29
    • Related Report
      2013 Annual Research Report
  • [Presentation] クリック化学により染色体DNA のマルチカラーイメージング2013

    • Author(s)
      劉 泓汕, 徐 岩
    • Organizer
      日本化学会第93回春季年会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県草津市)
    • Year and Date
      2013-03-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工制限酵素ARCUTを用いたヒト細胞内における相同組換え2013

    • Author(s)
      池戸 彰之 , 鴫 成実 , 須磨岡 淳 , 小宮山 眞
    • Organizer
      日本化学会第93春季年会
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス(滋賀県)
    • Year and Date
      2013-03-22
    • Related Report
      2012 Annual Research Report
  • [Presentation] Chromosome painting by using click chemistry : A light-up approach2013

    • Author(s)
      Takumi Ishizuka, Yan Xu
    • Organizer
      The 40th International Symposium on Nucleic Acids Chemistry(第40回国際核酸化学シンポジウム)
    • Place of Presentation
      神奈川大学(神奈川県横浜市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Manipulation of Human Genome Using Completely Chemistry-Based DNA Cutter2012

    • Author(s)
      小宮山眞
    • Organizer
      BIO'12 8th WSEAS International Conference on CELLULAR and MOLECULAR BIOLOGY,BIOPHYSICS and BIOENGINEERING
    • Place of Presentation
      モントルー(スイス)
    • Year and Date
      2012-12-29 – 2012-12-31
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Site-specific Scission and Recombination of Human Mitochondrial DNA by Using Artificial Restriction DNA Cutter2012

    • Author(s)
      鴫(日野) 成実 , 春元 俊正 , 小宮山 眞
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡)
    • Year and Date
      2012-12-11 – 2012-12-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] PNA/DNA Hybrid G-Quadruplex Formation as a Novel Approach2012

    • Author(s)
      Takumi Ishizuka , Yan Xu , Makoto Komiyama
    • Organizer
      ISNAC2012  第39回国際核酸化学シンポジウム
    • Place of Presentation
      名古屋大学豊田講堂(愛知県)
    • Year and Date
      2012-11-15 – 2012-11-17
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structure and Biological Function of Telomere RNA2012

    • Author(s)
      徐 岩
    • Organizer
      第39回国際核酸化学シンポジウム
    • Place of Presentation
      名古屋大学豊田講堂(愛知県)
    • Year and Date
      2012-11-15
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトテロメアDNAとRNAによるハイブリッド四重鎖の形成およびその生化学機能2012

    • Author(s)
      徐 岩
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学高等教育推進機構
    • Year and Date
      2012-09-07
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工DNAカッター(ARCUT)による細胞内DNA切断2012

    • Author(s)
      愛場 雄一郎 , 本田 祐太 , 韓 悦 , 亀島 渡 , 濱野 悠也 , 須磨岡 淳 , 鴫 成実 , 小宮山 眞
    • Organizer
      第6回バイオ関連化学シンポジウム
    • Place of Presentation
      北海道大学高等教育推進機構(北海道)
    • Year and Date
      2012-09-06
    • Related Report
      2012 Annual Research Report
  • [Presentation] PNAへのNLS修飾によるインベージョンの高効率化とDNA切断への応用2012

    • Author(s)
      愛場 雄一郎 , 本田 祐太 , 亀島 渡 , 濱野 悠 , Accetta Alessandro , Sforza Stefano , Marchelli Rosangela , Corradini Roberto , 小宮山 眞
    • Organizer
      第22回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学先端科学技術研究センター(東京)
    • Year and Date
      2012-06-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] 人工制限酵素を用いた遺伝子ノックアウト法の開発2011

    • Author(s)
      伊藤健一郎, 小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] PNA Peptide eonjugateを利用したDNA位置選択的切断2011

    • Author(s)
      愛場雄一郎、小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単分子検出デバイスとしての可動式DNAオリガミ2011

    • Author(s)
      葛谷明紀、小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] アロステリック酵素を模倣した可動式DNAオリガミによる高感度単分子検出2011

    • Author(s)
      葛谷明紀、小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトテロメアRNAの構造と生化学機能について2011

    • Author(s)
      徐岩、小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] ARCUTによる単一染色体のテロメア長測定2011

    • Author(s)
      石塚匠、小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 骨格中へのリンカー導入によるPNAのミスマッチ識別能向上2011

    • Author(s)
      西山友加里、小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 化学ツールを活用したゲノム工学の創成2011

    • Author(s)
      小宮山眞
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川大学(東日本大震災の影響で)(紙面・Web上での開催のみ)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pinpoint Manipulation of Human Genome Using Artificial DNA Cutter2011

    • Author(s)
      M.Komiyama
    • Organizer
      G-COE symposium in Nagoya on Biochemistry and Bionanotechnology
    • Place of Presentation
      名古屋
    • Year and Date
      2011-01-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] ナノメカニカルDNAオリガミデバイスは"単分子"ビーコンとして機能する2011

    • Author(s)
      葛谷明紀、小宮山眞
    • Organizer
      第13回生命化学研究会シンポジウム(冬季)
    • Place of Presentation
      東北大学
    • Year and Date
      2011-01-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Manipulation of Huge DNA by Artificial Restriction DNA Cutter (ARCUT).2011

    • Author(s)
      M. Komiyama
    • Organizer
      6th Cambridge Symposium on Nucleic Acids Chemistry and Biology
    • Place of Presentation
      Cambridge (UK)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Artificial DNA cutter to manipulate huge genome.2011

    • Author(s)
      M. Komiyama
    • Organizer
      15th Symposium on Chemistry of Nucleic Acid Compounds
    • Place of Presentation
      Cesky Krumlov (Czech Republic)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Genome Manipulation Using Chemistry-based Artificial Restriction DNA Cutters2011

    • Author(s)
      M.Komiyama
    • Organizer
      6th Cambridge Symposium on Nucleic Acids Chemistry and Biology
    • Place of Presentation
      Queens College, Cambridge, UK
    • Related Report
      2010 Annual Research Report
  • [Presentation] Site-selective scission of double-stranded DNA by Ce(IV)/EDTA and only one mixed-base PNA2010

    • Author(s)
      Yuichiro Aiba, Makoto Komiyama
    • Organizer
      PACIFICHEM 2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Strict recognition of double-stranded DNA by chemically modified PNA (Peptide Nucleic Acid)2010

    • Author(s)
      Yuichiro Aiba, Makoto Komiyama
    • Organizer
      PACIFICHEM 2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Artificial restriction DNA cutter as homologous recombination stimulator for genome engineering2010

    • Author(s)
      Hitoshi Katada, Makoto Komiyama
    • Organizer
      PACIFICHEM 2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] G-Rich Sequence-Specific Cleaving by PNA/DNA Hybrid G-Quadruplex Formation2010

    • Author(s)
      Takumi Ishizuka, Makoto Komiyama
    • Organizer
      PACIFICHEM 2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 人工制限酵素ARCUTによるヒトゲノムの遺伝子ターゲティング法の開発2010

    • Author(s)
      鴫(日野)成実, 小宮山眞
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] 人工制限酵素を用いたヒト生細胞内でのゲノムの位置特異的切断法の開発2010

    • Author(s)
      野口恵理, 小宮山眞
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] Incorporation of a flexible linker into pseudo-complementary PNA for the strict recognition of DNA2010

    • Author(s)
      Yuichiro Aiba, Makoto Komiyama
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry 2010
    • Place of Presentation
      Yokohama
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Artificial restriction DNA cutter for targeted gene knockout2010

    • Author(s)
      Kenichiro Ito, Makoto Komiyama
    • Organizer
      The 37th International Symposium on Nucleic Acids Chemistry 2010
    • Place of Presentation
      Yokohama
    • Year and Date
      2010-11-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 化学的アプローチによるヒトテロメアRNA構造と機能の解析2010

    • Author(s)
      徐岩
    • Organizer
      第4回バイオ関連化学合同シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単一のPNA-peptideコンジュゲートを利用した位置選択的DNA切断2010

    • Author(s)
      愛場雄一郎、小宮山眞
    • Organizer
      第4回バイオ関連化学合同シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA Origamiの構造変化に基づく非共有結合形成の単分子検出2010

    • Author(s)
      酒井雄介、小宮山眞
    • Organizer
      第4回バイオ関連化学シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 人工制限酵素を用いたヒト細胞おけるジーンターゲティング法の開発2010

    • Author(s)
      鴫成実, 小宮山眞, 須磨岡淳
    • Organizer
      第4回バイオ関連化学合同シンポジウム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 単一のPNA-peptideコンジュゲートを利用した位置選択的DNA切断2010

    • Author(s)
      愛場雄一郎、小宮山眞
    • Organizer
      第25回生体機能関連化学シンポジウム若手フォーラム
    • Place of Presentation
      大阪大学
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 人工制限酵素による相同組換えの促進とゲノム工学への応用2010

    • Author(s)
      堅田仁、小宮山眞
    • Organizer
      第20回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学
    • Year and Date
      2010-07-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトテロメアRNAの構造と生化学機能2010

    • Author(s)
      徐岩
    • Organizer
      第12回日本RNA学会年会
    • Place of Presentation
      一橋記念講堂
    • Year and Date
      2010-07-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] 機能性リンカー導入による新規PNA修飾法の開発2010

    • Author(s)
      愛場雄一郎、小宮山眞
    • Organizer
      第22回生体機能関連化学部会若手の会サマースクール
    • Place of Presentation
      三重県
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] DNA hydrolysis by Ce(IV) complex prepared from Ce(III) precursor2010

    • Author(s)
      M.Komiyama
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Artificial restriction DNA cutter (ARCUT) for homologous recombination in human cells2010

    • Author(s)
      M.Komiyama
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies
    • Place of Presentation
      Hawaii, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Artificial conversion of a gene in human genome2010

    • Author(s)
      M.Komiyama
    • Organizer
      Workshop on Nucleic Acid Chemistry
    • Place of Presentation
      Telluride, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Chemistry-based restriction DNA cutter for genome manipulation

    • Author(s)
      M. Komiyama
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] 人工制限酵素による細胞内ゲノム改変

    • Author(s)
      多賀 亮介
    • Organizer
      日本化学会 第92春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Gene targeting in human cells using Artificial Restriction DNA CUTter (ARCUT)

    • Author(s)
      Narumi Shigi
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Site-specific scission and recombination of mitochondrial DNA by using Artificial restriction DNA cutter

    • Author(s)
      Toshimasa Harumoto
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(札幌)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトテロメアRNAの構造と生化学機能およびテロメアRNAを標的とする小分子の探索

    • Author(s)
      徐 岩
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(茨城)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Ce(IV)/EDTA とペプチド核酸・ペプチドコンジュゲートによる位置選択的 DNA 切断

    • Author(s)
      愛場 雄一郎
    • Organizer
      第28回希土類討論会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内応用を志向した人工DNAカッター(ARCUT)の開発

    • Author(s)
      愛場 雄一郎
    • Organizer
      第21回バイオ・高分子シンポジウム
    • Place of Presentation
      東京大学先端科学技術研究センター(東京都)
    • Related Report
      2011 Annual Research Report
  • [Presentation] PNA-peptide conjugateの2重鎖DNA位置選択的切断への応用

    • Author(s)
      愛場 雄一郎
    • Organizer
      第60回高分子討論会
    • Place of Presentation
      岡山大学 津島キャンパス(岡山県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ナノメカニカル DNAオリガミデバイスのアロステリック構造制御による高感度単分子検出

    • Author(s)
      葛谷 明紀
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞内応用へ向けた人工DNAカッターARCUTの高機能化

    • Author(s)
      愛場 雄一郎
    • Organizer
      第5回バイオ関連化学シンポジウム
    • Place of Presentation
      つくば国際会議場(茨城県)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Nanomechanical DNA Origami Devices as“ Single-Molecule Beacons”

    • Author(s)
      Akinori Kuzuya
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Site-selective scission of double-stranded DNA by using PNA-peptide conjugate

    • Author(s)
      Wataru Kameshima
    • Organizer
      The 38th International Symposium on Nucleic Acid Chemistry
    • Place of Presentation
      北海道大学(北海道)
    • Related Report
      2011 Annual Research Report
  • [Book] "Covalent and non-covalent conjugates of oligonucleotide as artificial restriction DNA cutters" In Keith R Fox (Eds.) "DNA Conjugates and Sensors"2012

    • Author(s)
      Makoto Komiyama
    • Publisher
      Royal Society of Chemistry
    • Related Report
      2011 Annual Research Report
  • [Book] 「人工酵素の歴史と将来」「酵素利用技術体系(小宮山眞監修)」2010

    • Author(s)
      小宮山眞
    • Publisher
      NTS
    • Related Report
      2010 Annual Research Report
  • [Book] 化学修飾による特異性の改変」、「酵素利用技術体系(小宮山眞監修)」2010

    • Author(s)
      堅田仁
    • Publisher
      NTS
    • Related Report
      2010 Annual Research Report
  • [Book] 「スーパー人工酵素」「酵素利用技術体系(小宮山眞監修)」2010

    • Author(s)
      須磨岡淳
    • Publisher
      NTS
    • Related Report
      2010 Annual Research Report
  • [Remarks] 筑波大学生命領域学際研究センター プロジェクト

    • URL

      http://www.tara.tsukuba.ac.jp/project/project09.html

    • Related Report
      2013 Annual Research Report
  • [Remarks] 筑波大学 生命領域学際研究センター プロジェクト

    • URL

      http://www.tara.tsukuba.ac.jp/project/project09.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 小宮山研究室HP

    • URL

      http://www.mkomi.rcast.u-tokyo.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.mkomi.rcast.u-tokyo.ac.jp/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2022-11-04  

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