Mechanisms underlying intracortical distribution of inhibitory interneurons and their synaptic connections

• Project/Area Number: 22220004
• Research Category: Grant-in-Aid for Scientific Research (S)
• Allocation Type: Single-year Grants
• Research Field: Neuroscience in general
• Research Institution: Osaka University
• Principal Investigator: Murakami Fujio 大阪大学, 生命機能研究科, 名誉教授 (20089882)
• Co-Investigator(Kenkyū-buntansha): SONG Wen-Jie 熊本大学, 大学院生命科学研究部, 教授 (90216573) ; YAMAMOTO Nobuhiko 大阪大学, 大学院生命機能研究科, 教授 (00191429) ; 田辺 康人 京都大学, 医学研究科, 医員 (10311309)
• Project Period (FY): 2010-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥216,320,000 (Direct Cost: ¥166,400,000、Indirect Cost: ¥49,920,000); Fiscal Year 2014: ¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000); Fiscal Year 2013: ¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000); Fiscal Year 2012: ¥42,510,000 (Direct Cost: ¥32,700,000、Indirect Cost: ¥9,810,000); Fiscal Year 2011: ¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000); Fiscal Year 2010: ¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
• Keywords: 大脳皮質 / 抑制性介在ニューロン / 移動の終了 / 皮質内最終分布 / 極性獲得 / 子宮内電気穿孔法 / 尾側基底核原基 / 層分布 / 内側基底核原基 / nkx2.1 / Sp8 / 電気穿孔法 / 移動能 / 内在的制御 / GAD67-GFPマウス / 皮質板 / 放射状グリア細胞 / 分子・細胞神経科学 / 神経細胞移動 / in vivoイメージング / 抑制性介在ニューロ

Outline of Final Research Achievements

The present study was performed to gain insight into the mechanisms of migration termination and final distribution of cortical interneurons (CIs) within the neocortex. To this end we developed a system in which migrating CIs can be observed in living embryos. We found that the CIs migrate in all directions in the marginal zone (MZ) and medially in the intermediate/subventricular zones. We also found that the termination of migration is regulated both by intrinsic and extrinsic mechanisms using time-lapse imaging of dissociated CIs and cortical explants. At the final settlement CIs extend axons and dendrites. We carried out time-lapse imaging for days and found the polarity of migrating CIs is not inherited by mature neurons but rather they acquire a new polarity de novo. There is a variety of subtypes of CIs and each of them shows intracortical distribution of its own. We found that random walk-like migration of CIs in the MZ is required for the final distribution of CIs subtypes.

Assessment Rating

Verification Result (Rating)

A

Research Products

• [Journal Article] Evidence That the Laminar Fate of LGE/CGE-Derived Neocortical Interneurons Is Dependent on Their Progenitor Domains. (2016)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Toshiya Kimura, Yo Uemura, Fujio Murakami
  • Journal Title: The Journal of neuroscience Volume: 36 Issue: 6 Pages: 2044-2056
  • DOI: 10.1523/jneurosci.3550-15.2016
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] From migration to settlement: the pathways, migration modes and dynamics of neurons in the developing brain (2016)
  • Author(s): Yumiko Hatanaka, Yan Zhu, Makio Torigoe, Yoshiaki Kita, Fujio Murakami
  • Journal Title: Proceedings of the Japan Academy, Series B Volume: 92 Issue: 1 Pages: 1-19
  • DOI: 10.2183/pjab.92.1
  • NAID: 130005118553
  • ISSN: 0386-2208, 1349-2896
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Chemokine Signaling Controls Integrity of Radial Glial Scaffold in Developing Spinal Cord and Consequential Proper Position of Boundary Cap Cells. (2016)
  • Author(s): Yan Zhu, Tomoko Matsumoto, Takashi Nagasawa, Fabienne Mackay, Fujio Murakami
  • Journal Title: The Journal of neuroscience Volume: 35 Issue: 24 Pages: 9211-9224
  • DOI: 10.1523/jneurosci.0156-15.2015
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium. (2015)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Yan Zhu, Hiroaki Kobayashi, Fujio Murakami
  • Journal Title: Scientific reports Volume: 5 Issue: 1 Pages: 07-07
  • DOI: 10.1038/srep12258
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Calm1 signaling pathway is essential for the migration of mouse precerebellar neurons (2015)
  • Author(s): Hiroaki Kobayashi, Shunsuke Saragai, Atsushi Naito, Koji Ichio, Daisuke Kawauchi, Fujio Murakami
  • Journal Title: Development Volume: 142 Pages: 375-384
  • DOI: 10.1242/dev.112680
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Specific labeling of climbing fibers shows early synaptic interactions with immature Purkinje cells in the prenatal cerebellum. (2014)
  • Author(s): Yoshiaki Kita, Kazuto Tanaka, Fujio Murakami
  • Journal Title: Dev Neurobiol Volume: 75 Issue: 9 Pages: 927-934
  • DOI: 10.1002/dneu.22259
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evidence that dendritic mitochondria negatively regulate dendritic branching in pyramidal neurons in the neocortex. (2014)
  • Author(s): Toshiya Kimura, Fujio Murakami
  • Journal Title: The Journal of neuroscience Volume: 34 Issue: 20 Pages: 6938-6951
  • DOI: 10.1523/jneurosci.5095-13.2014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Role of neuropilin-2 in the ipsilateral growth of midbrain dopaminergic axons (2013)
  • Author(s): Makio Torigoe, Kenta Yamauchi, Atsushi Tamada, Ikuo Matsuda, Atsu Aiba, Valérie Castellani, Fujio Murakami
  • Journal Title: Eur J Neurosci. Volume: 37 Issue: 10 Pages: 1573-83
  • DOI: 10.1111/ejn.12190
  • Peer Reviewed
• [Journal Article] Four-dimensional analysis of nucleogenesis of the pontine nucleus in the hindbrain (2013)
  • Author(s): Masaki Shinohara, Yan Zhu, Fujio Murakami
  • Journal Title: The Journal of comparative neurology Volume: 521 Issue: 14 Pages: 3340-57
  • DOI: 10.1002/cne.23353
  • Peer Reviewed
• [Journal Article] Development of Cerebellar Neurons and Glias Revealed by in Utero Electroporation: Golgi-Like Labeling of Cerebellar Neurons and Glias (2013)
  • Author(s): Kita, Y., Kawakami, K., Takahashi, Y. and *Murakami, F.
  • Journal Title: PLoS ONE Volume: 8 (7) Issue: 7 Pages: e70091-e70091
  • DOI: 10.1371/journal.pone.0070091
  • Peer Reviewed / Open Access
• [Journal Article] N-Cadherin Sustains Motility and Polarity of Future Cortical Interneurons during Tangential Migration (2013)
  • Author(s): Camilla Luccardini, Laetitia Hennekinne, Lucie Viou, Mitsutoshi Yanagida, Fujio Murakami, Nicoletta Kessaris, Xufei Ma, Robert S Adelstein, René-Marc Mège, Christine Métin
  • Journal Title: J. Neurosci Volume: 33 Issue: 46 Pages: 18149-60
  • DOI: 10.1523/jneurosci.0593-13.2013
  • Peer Reviewed
• [Journal Article] The control of precerebellar neuron migration by RNA-binding protein Csde1. (2013)
  • Author(s): H Kobayashi, D Kawauchi, Y Hashimoto, T Ogata, F Murakami
  • Journal Title: Neuroscience Volume: 253 Pages: 292-303
  • DOI: 10.1016/j.neuroscience.2013.08.055
  • Peer Reviewed
• [Journal Article] Temporally- and Spatially-Regulated Generation of Distinct Descendants by Sonic Hedgehog-Expressing Progenitors in the Forebrain (2012)
  • Author(s): Yukiko Wada
  • Journal Title: Dev Neurobiol. Volume: 29 Pages: 2649-1659
  • Peer Reviewed
• [Journal Article] Temporal sequence of visuo-auditory interaction in multiple areas of the guinea pig visual cortex (2012)
  • Author(s): Nishimura M.
  • Journal Title: PLOS ONE Volume: 7 (9)
  • Peer Reviewed
• [Journal Article] Deficiency of sphingomyelin synthase-1 but not sphingomyelin synthase-2 causes hearing impairments in mice. (2012)
  • Author(s): Lu MH
  • Journal Title: J.Physiol Volume: 590 Pages: 4029-4044
  • Peer Reviewed
• [Journal Article] Role of Neph2 in pontine nuclei formation in the developing hindbrain. (2011)
  • Author(s): Kazuhiko Nishida
  • Journal Title: Mol Cell Neurosci Volume: 46 Pages: 662-670
  • Peer Reviewed
• [Journal Article] Identification and characterization of an insular auditory field in mice. (2011)
  • Author(s): Sawatari H.
  • Journal Title: Eur J Neurosci Volume: 34 Pages: 1944-1952
  • Peer Reviewed
• [Journal Article] A train of electrical pulses applied to the primary auditory cortex evokes a conditioned response in guinea pigs. (2011)
  • Author(s): Okuda Y.
  • Journal Title: Neurocsi Res Volume: 71 Pages: 103-106
  • Peer Reviewed
• [Journal Article] Voltage-gated K+ channel KCNQ1 regulates insulin secretion in MIN6 β-cell line. (2011)
  • Author(s): Yamagata K
  • Journal Title: BBRC Volume: 407 Pages: 620-625
  • Peer Reviewed
• [Journal Article] Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. (2011)
  • Author(s): Yano M.
  • Journal Title: J.Biol. Chem Volume: 286 Pages: 3992-4002
  • Peer Reviewed
• [Presentation] ネトリン1欠損マウスにおける後脳交連軸索の末梢への伸長 (2012)
  • Author(s): 山内 健太
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 中脳ドーパミン作動性ニューロン軸索の非交差性投射におけるニューロピリン２の役割 (2012)
  • Author(s): 鳥越 万紀夫
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 小脳脳室帯・菱脳唇由来細胞の分布パターン (2012)
  • Author(s): 喜多 善亮
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Migration, its Termination, Axon Initiation and Maturation of Cortical Interneurons (2012)
  • Author(s): 村上 富士夫
  • Organizer: 第59回NIBB CONFERENCE "Neocortical Organization"
  • Place of Presentation: 岡崎
  • Invited
• [Presentation] Formation of precerebellar nuclei in the hindbrain (2011)
  • Author(s): Fujio Murakami
  • Organizer: Building a Functional Brain
  • Place of Presentation: The University of Auckland (New Zealand)(招待講演)
  • Year and Date: 2011-01-29
• [Presentation] 内在性要因と環境が大脳皮質抑制性ニューロンの移動の停止を調節する (2011)
  • Author(s): 稲村 直子
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] 線条体striosome/matrix構造形成過程におけるmedium spiny neuronの発生・発達様式 (2011)
  • Author(s): 萩本 和也
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] A chemokine receptor CXCR7 non-cell-autonomously controls migration and nuclei formation of pontine neurons (2011)
  • Author(s): Yan Zhu
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
• [Presentation] Intracortical Migration of GABAergic Interneurons and Morphological Changes during Early Stages of Maturation (2010)
  • Author(s): Fujio Murakami
  • Organizer: QBI-Osaka Developmental Neurobiology Workshop
  • Place of Presentation: The University of Queensland (AUSTRALIA)(招待講演)
  • Year and Date: 2010-09-13
• [Remarks] 脳ができあがるまで（基本編）
  • URL: http://square.umin.ac.jp/murakami-lab/ippan/website%20files/home_wbs.html
• [Remarks] 神経細胞が軸索を伸ばすとき、過渡的にウニのような形態を示す
  • URL: http://www.fbs.osaka-u.ac.jp/gcoe/jp/events/achievement/cortical/
• [Remarks] 神経細胞の移動する様子を生きたﾏｳｽ胎仔で観察することに成功ー胎生期における脳形成の理解に貢献ー
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/09/20120925_1
• [Remarks] 脳ができあがるまで（基本編）
  • URL: http://square.umin.ac.jp/murakami-lab/ippan/website%20files/home_wbs.html
• [Remarks] 神経細胞が軸索を伸ばすとき、過渡的にウニのような形態を示す
  • URL: http://www.fbs.osaka-u.ac.jp/gcoe/jp/events/achievement/cortical/
• [Remarks] 神経細胞の移動する様子を生きたﾏｳｽ胎仔で観察することに成功ー胎生期における脳形成の理解に貢献ー
  • URL: http://www.osaka-u.ac.jp/ja/news/ResearchRelease/2012/09/20120925_1